Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,842,141	A→G	intergenic (‑6/‑230)	ynjH ← / → gdhA	DUF1496 family protein/glutamate dehydrogenase, NADP‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,842,141	0	A	G	100.0%	237.0 / NA	67	intergenic (‑6/‑230)	ynjH/gdhA	DUF1496 family protein/glutamate dehydrogenase, NADP‑specific
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/34);  total (33/34)

TGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTCCTGGGTCA  >  NC_000913/1842008‑1842260                                                                                                                                      |                                                                                                                        tGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCt                                                                                                                    <  1:631276/139‑1 (MQ=255) tGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCt                                                                                                                    >  1:926268/1‑139 (MQ=255)  gcgcTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGTTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCtt                                                                                                                   <  2:706629/139‑1 (MQ=255)   cgcTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTCCGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGTACTGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGATCCTTa                                                                                                                  >  2:370104/1‑139 (MQ=255)   cgcTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTa                                                                                                                  >  1:618065/1‑139 (MQ=255)   cgcTGACTACCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTa                                                                                                                  >  1:665776/1‑139 (MQ=255)           gCCCGCCTGAACTGAACTCTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTAtt                                                                                                          <  1:370104/139‑1 (MQ=255)               gCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGtct                                                                                                      <  1:1048256/139‑1 (MQ=255)                  tgaactgaacACTTCTGGCTGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTg                                                                                                   <  2:665776/139‑1 (MQ=255)                  tgaactgaacACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTg                                                                                                   <  1:845261/139‑1 (MQ=255)                     actgaacACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTa                                                                                                <  2:618065/139‑1 (MQ=255)                                        aCGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGt                                                                             >  1:112352/1‑139 (MQ=255)                                        aCGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGt                                                                             >  2:216766/1‑139 (MQ=255)                                                 tCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTATGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACt                                                                    >  1:376504/1‑139 (MQ=255)                                                     cGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAGATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAg                                                                >  2:293298/1‑139 (MQ=255)                                                     cGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAg                                                                >  2:501990/1‑139 (MQ=255)                                                         cGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTa                                                                                                                  >  2:457811/1‑85 (MQ=255)                                                         cGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTa                                                                                                                  <  1:457811/85‑1 (MQ=255)                                                                tAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTa                                                     >  2:987833/1‑139 (MQ=255)                                                                tAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTa                                                     <  1:216766/139‑1 (MQ=255)                                                                      gggTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCAt                                               <  1:501990/139‑1 (MQ=255)                                                                      gggTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCAt                                               <  1:987833/139‑1 (MQ=255)                                                                        gTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCt                                                                                                 >  1:317740/1‑87 (MQ=255)                                                                        gTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCt                                                                                                 <  2:317740/87‑1 (MQ=255)                                                                               aGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATg                                      <  1:1342934/139‑1 (MQ=255)                                                                                 tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCa                                                                         >  1:204723/1‑102 (MQ=255)                                                                                 tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCa                                                                         <  2:204723/102‑1 (MQ=255)                                                                                 tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGt                                                             <  1:141641/114‑1 (MQ=255)                                                                                 tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGt                                                             >  2:141641/1‑114 (MQ=255)                                                                                 tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCt                                                           >  1:114991/1‑116 (MQ=255)                                                                                 tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCt                                                           <  2:114991/116‑1 (MQ=255)                                                                                  gCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCa                                                >  1:92529/1‑126 (MQ=255)                                                                                  gCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCa                                                <  2:92529/126‑1 (MQ=255)                                                                                       tAATGGAGATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTAct                              <  1:293298/139‑1 (MQ=255)                                                                                            gAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGt                         <  2:112352/139‑1 (MQ=255)                                                                                              aaTGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTAt                                                                                                           >  1:984394/1‑55 (MQ=255)                                                                                              aaTGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTAt                                                                                                           <  2:984394/55‑1 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTa                                                                                                >  1:268278/1‑53 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTa                                                                                                <  2:268278/53‑1 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTa                                                                 >  2:405889/1‑84 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTa                                                                 <  1:405889/84‑1 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGt                                                             >  1:951759/1‑88 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGtt                                                      <  1:307658/95‑1 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGtt                                                      >  2:307658/1‑95 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTg               <  1:1240475/134‑1 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTg               >  2:1240475/1‑134 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTc          >  1:474405/1‑139 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTc          >  1:278551/1‑139 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTc          >  2:421467/1‑139 (MQ=255)                                                                                                           cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTCTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGt                                                             <  2:951759/88‑1 (MQ=255)                                                                                                            ggCGAACAATGCTCGACTCACAGGGAGCTCCTTAAc                                                                                                                <  2:282865/36‑1 (MQ=255)                                                                                                            ggCGAACAATGCTCGACTCACAGGGAGCTCCTTAAc                                                                                                                >  1:282865/1‑36 (MQ=255)                                                                                                            ggCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTg               >  2:822922/1‑133 (MQ=255)                                                                                                            ggCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTg               <  1:822922/133‑1 (MQ=255)                                                                                                              cGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCAt                                               <  2:1216408/99‑1 (MQ=255)                                                                                                              cGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCAt                                               >  1:1216408/1‑99 (MQ=255)                                                                                                               gAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTCCTgg      <  2:376504/139‑1 (MQ=255)                                                                                                                aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            >  1:14708/1‑116 (MQ=255)                                                                                                                aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            <  1:372441/116‑1 (MQ=255)                                                                                                                aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            >  2:372441/1‑116 (MQ=255)                                                                                                                aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            <  2:14708/116‑1 (MQ=255)                                                                                                                aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGttatta                    <  1:1258516/124‑1 (MQ=255)                                                                                                                aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGttatta                    >  2:1258516/1‑124 (MQ=255)                                                                                                                   aaTGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTCCTGGGTCa  >  1:344847/1‑139 (MQ=255)                                                                                                                               acaGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTg                                                          <  1:124419/71‑1 (MQ=255)                                                                                                                               acaGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTg                                                          >  2:124419/1‑71 (MQ=255)                                                                                                                               acaGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            >  2:509504/1‑101 (MQ=255)                                                                                                                               acaGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            <  1:509504/101‑1 (MQ=255)                                                                                                                                      |                                                                                                                        TGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTCCTGGGTCA  >  NC_000913/1842008‑1842260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑