Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,078	C→T	*35* (TAG→TAA)	yoaI ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,078	0	C	T	100.0%	142.8 / NA	45	*35* (TAG→TAA)	yoaI	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/23);  total (22/23)

TCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAG  >  NC_000913/1873948‑1874202                                                                                                                                   |                                                                                                                             tctACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGt                                                                                                                      >  1:369550/1‑139 (MQ=255)    aCTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCg                                                                                                                   >  1:157095/1‑139 (MQ=255)     ctctTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGt                                                                                                                  <  2:157095/139‑1 (MQ=255)     ctctTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGt                                                                                                                  <  2:369550/139‑1 (MQ=255)          cGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTA‑TTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATa                                                                                                            >  1:783421/1‑139 (MQ=255)           gAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGCATTATTTGCGCATTGTGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATa                                                                                                            >  2:618947/1‑139 (MQ=255)             aCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATaag                                                                                                          >  2:316324/1‑139 (MQ=255)                aTTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATaagaag                                                                                                       >  1:1192425/1‑139 (MQ=255)                      tgaAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTA‑TTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                <  2:783421/139‑1 (MQ=255)                           tCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACaaaa                                                                                            >  1:782886/1‑139 (MQ=255)                               cTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAg                                                                                        <  2:1192425/139‑1 (MQ=255)                               cTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAg                                                                                        >  1:809325/1‑139 (MQ=255)                                  cAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTac                                                                                     <  2:1273365/139‑1 (MQ=255)                                         ggCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCa                                                                              >  2:349184/1‑139 (MQ=255)                                                       aTCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGa                                                                >  1:643412/1‑139 (MQ=255)                                                        tCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGAt                                                               <  1:360869/139‑1 (MQ=255)                                                         ccGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATg                                                              <  2:809325/139‑1 (MQ=255)                                                          cGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACg                                                                 <  2:605029/135‑1 (MQ=255)                                                          cGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACg                                                                 >  1:605029/1‑135 (MQ=255)                                                                         cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGt                                                                                                                      <  2:1145634/67‑1 (MQ=255)                                                                         cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGt                                                                                                                      >  1:1145634/1‑67 (MQ=255)                                                                           cgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                <  1:486174/87‑1 (MQ=255)                                                                           cgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                >  2:486174/1‑87 (MQ=255)                                                                               ttattTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                        <  1:316324/139‑1 (MQ=255)                                                                                tattTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACa                                       <  2:643412/139‑1 (MQ=255)                                                                                        cattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAgtg                                               <  2:93527/123‑1 (MQ=255)                                                                                        cattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAgtg                                               >  1:93527/1‑123 (MQ=255)                                                                                          ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCa                                                                              >  1:1234831/1‑90 (MQ=255)                                                                                          ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCa                                                                              <  2:1234831/90‑1 (MQ=255)                                                                                              gcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGtt                         <  2:1001178/139‑1 (MQ=255)                                                                                              gcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGtt                         <  1:349184/139‑1 (MQ=255)                                                                                                    aGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGc                   <  2:782886/139‑1 (MQ=255)                                                                                                           gTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAACCATTTGATCGTTCATAGc                   <  2:746798/132‑1 (MQ=255)                                                                                                           gTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGc                   >  1:746798/1‑132 (MQ=255)                                                                                                            tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                >  2:455004/1‑54 (MQ=255)                                                                                                            tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                <  1:455004/54‑1 (MQ=255)                                                                                                            tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGa                              >  2:236594/1‑120 (MQ=255)                                                                                                            tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGa                              <  1:236594/120‑1 (MQ=255)                                                                                                            tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGtt                         >  1:784681/1‑125 (MQ=255)                                                                                                            tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGtt                         <  2:784681/125‑1 (MQ=255)                                                                                                            tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGt                          >  2:340146/1‑124 (MQ=255)                                                                                                            tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGt                          <  1:340146/124‑1 (MQ=255)                                                                                                                tttCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGga       >  2:108117/1‑139 (MQ=255)                                                                                                                              cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAg  >  2:822414/1‑130 (MQ=255)                                                                                                                              cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGGTCATAGCATGCCCCCGCGAGATAg  <  1:822414/130‑1 (MQ=255)                                                                                                                                   |                                                                                                                             TCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAG  >  NC_000913/1873948‑1874202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑