Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,980,188 C→T *475* (TAG→TAA)  otsA ← trehalose‑6‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,980,1880CT100.0% 138.1 / NA 44*475* (TAG→TAA) otsAtrehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/25);  total (19/25)

TCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCA  >  NC_000913/1980055‑1980318
                                                                                                                                     |                                                                                                                                  
tcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGAGAGATTAACCGCTCTTTCGc                                                                                                                               <  1:914124/139‑1 (MQ=255)
 catTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCa                                                                                                                              <  1:18981/139‑1 (MQ=255)
             gTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAg                                                                                                                  >  2:733855/1‑139 (MQ=255)
               gATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGt                                                                                                                <  1:1109851/139‑1 (MQ=255)
                 tttGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAg                                                                                                              <  2:570826/139‑1 (MQ=255)
                           tcacaatcacaGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATc                                                                                                    <  1:1310588/139‑1 (MQ=255)
                                      gTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCgctg                                                                                              >  1:1093977/1‑134 (MQ=255)
                                      gTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCgctg                                                                                              <  2:1093977/134‑1 (MQ=255)
                                         tCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCttt                                                                                      >  1:789739/1‑139 (MQ=255)
                                                gcgAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCt                                                                               <  2:789739/139‑1 (MQ=255)
                                                   aGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcg                                                                            <  2:97200/139‑1 (MQ=255)
                                                   aGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcg                                                                            >  2:1128045/1‑139 (MQ=255)
                                                      aTCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg                                                                         <  1:733855/139‑1 (MQ=255)
                                                            cTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAt                                                                   <  1:529188/139‑1 (MQ=255)
                                                                   aaaaGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCttt                                                            <  1:1128045/139‑1 (MQ=255)
                                                                                  tCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGc                                                                                                                               >  2:59643/1‑57 (MQ=255)
                                                                                  tCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGc                                                                                                                               <  1:59643/57‑1 (MQ=255)
                                                                                  tCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAg                                             >  1:1035956/1‑139 (MQ=255)
                                                                                    aaGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCa                                           >  2:877791/1‑139 (MQ=255)
                                                                                      gTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACt                                         <  1:1331529/139‑1 (MQ=255)
                                                                                               aTGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGa                                                                                                                     >  1:37692/1‑54 (MQ=255)
                                                                                               aTGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGa                                                                                                                     <  2:37692/54‑1 (MQ=255)
                                                                                               aTGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGt                                >  2:1339204/1‑139 (MQ=255)
                                                                                                tgtgCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTcc                                       <  1:80399/131‑1 (MQ=255)
                                                                                                tgtgCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTcc                                       >  2:80399/1‑131 (MQ=255)
                                                                                                   gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTgg                                                                                                                      <  1:630154/49‑1 (MQ=255)
                                                                                                   gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTgg                                                                                                                      >  2:630154/1‑49 (MQ=255)
                                                                                                     tGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa                                  <  1:1006582/131‑1 (MQ=255)
                                                                                                     tGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa                                  >  2:1006582/1‑131 (MQ=255)
                                                                                                         aGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTa                                                           >  2:1330794/1‑102 (MQ=255)
                                                                                                         aGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTa                                                           <  1:1330794/102‑1 (MQ=255)
                                                                                                             ccACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa                                  <  2:844334/123‑1 (MQ=255)
                                                                                                             ccACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa                                  >  1:844334/1‑123 (MQ=255)
                                                                                                                 ttACGGGAGATTGACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc                                            <  2:1263386/109‑1 (MQ=255)
                                                                                                                 ttACGGGAGATTGACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc                                            >  1:1263386/1‑109 (MQ=255)
                                                                                                                 ttACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc                                            >  1:1293298/1‑109 (MQ=255)
                                                                                                                 ttACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc                                            <  2:1293298/109‑1 (MQ=255)
                                                                                                                     gggAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATGGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAt                                                                   <  2:103973/82‑1 (MQ=255)
                                                                                                                     gggAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAt                                                                   >  1:103973/1‑82 (MQ=255)
                                                                                                                         gaTTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa                                  >  2:800934/1‑111 (MQ=255)
                                                                                                                         gaTTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa                                  <  1:800934/111‑1 (MQ=255)
                                                                                                                             aaCCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCa  <  1:111531/139‑1 (MQ=255)
                                                                                                                                 gCTCTTACGCAAGCTTTGGAAAGGCAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg                                                                         >  2:1092563/1‑64 (MQ=255)
                                                                                                                                 gCTCTTACGCAAGCTTTGGAAAGGCAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg                                                                         <  1:1092563/64‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                  
TCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCA  >  NC_000913/1980055‑1980318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑