Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,068,952 C→T *302* (TAG→TAA insD1 ← IS2 transposase TnpB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,068,9520CT98.8% 816.5 / ‑1.2 259*302* (TAG→TAAinsD1IS2 transposase TnpB
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (130/126);  total (132/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-01

Only 100 of 259 total aligned reads displayed.

ATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCC  >  NC_000913/2068818‑2069072
                                                                                                                                      |                                                                                                                        
aTTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtata                                                                                                                      >  1:1308479/1‑139 (MQ=255)
        acaaacaTTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGa                                                                                                              <  1:1017759/139‑1 (MQ=255)
         caaacaTTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAg                                                                                                               >  2:14692/1‑137 (MQ=255)
                tGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt                                                                                                      <  2:172847/139‑1 (MQ=255)
                       cTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACtt                                                                                               >  1:446253/1‑139 (MQ=255)
                          caccaATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAc                                                                                            >  1:521902/1‑139 (MQ=255)
                               aTGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGTCCTtat                                                                                                                       <  1:72973/107‑1 (MQ=255)
                               aTGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                >  2:686455/1‑130 (MQ=255)
                               aTGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                <  1:686455/130‑1 (MQ=255)
                                 gCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGa                                                                                                              <  2:151616/114‑1 (MQ=255)
                                    tCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           >  2:1195416/1‑130 (MQ=255)
                                      cATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGcc                                                                                >  1:82677/1‑139 (MQ=255)
                                       aTAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGccc                                                                               >  1:555079/1‑139 (MQ=255)
                                       aTAGCCGATAATTTGCAGGACATCCCCGGAGAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                >  1:1299513/1‑121 (MQ=255)
                                           ccGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                >  2:555388/1‑118 (MQ=255)
                                              aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            <  1:107162/103‑1 (MQ=255)
                                              aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                        >  1:964677/1‑139 (MQ=255)
                                               tAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGcc                                                                                >  1:891219/1‑130 (MQ=255)
                                               tAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGcc                                                                                <  2:891219/130‑1 (MQ=255)
                                                  tttGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCTCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGa                                                                    <  2:964677/139‑1 (MQ=255)
                                                     gCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGACAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGatat                                                                 >  1:55985/1‑139 (MQ=255)
                                                         gACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTccc                                                             <  2:446253/139‑1 (MQ=255)
                                                             tCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg                                                         >  2:1114625/1‑139 (MQ=255)
                                                             tCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg                                                         <  1:1224914/139‑1 (MQ=255)
                                                              ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGc                                                                       >  1:700818/1‑124 (MQ=255)
                                                              ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGc                                                                       <  2:700818/124‑1 (MQ=255)
                                                                   gAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                           >  2:282598/1‑83 (MQ=255)
                                                                     gTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTATTAACTTAACCCATTACAAGCCCGCTGCCGCTGATATACCCGTGGCGAGCGAt                                                 >  1:1004640/1‑139 (MQ=25)
                                                                      tAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                >  1:146411/1‑139 (MQ=255)
                                                                        gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTc                                                                                                                  >  2:1344417/1‑71 (MQ=255)
                                                                        gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            <  1:773494/77‑1 (MQ=255)
                                                                        gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAAc                                              >  2:1138765/1‑139 (MQ=255)
                                                                         agaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGAc                                                                                                             <  1:912455/75‑1 (MQ=255)
                                                                         agaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                           <  2:343672/77‑1 (MQ=255)
                                                                         agaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                           >  1:343672/1‑77 (MQ=255)
                                                                         agaTAACAGACAACTCCTTAGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGGTATGCCCTTAAATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGCGCGATAAcc                                             >  1:389713/1‑139 (MQ=21)
                                                                          gaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTta                                                                                                                        <  1:298715/63‑1 (MQ=255)
                                                                           aTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           >  1:1130360/1‑91 (MQ=255)
                                                                           aTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAAcccg                                           <  1:1187262/139‑2 (MQ=255)
                                                                             aaCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                <  2:612027/68‑1 (MQ=255)
                                                                             aaCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                >  1:612027/1‑68 (MQ=255)
                                                                             aaCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                >  1:1289444/1‑84 (MQ=255)
                                                                             aaCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgc                                         <  1:26875/139‑1 (MQ=255)
                                                                              aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGa                                                                                                              <  2:1293023/69‑1 (MQ=255)
                                                                              aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGa                                                                                                              >  1:1293023/1‑69 (MQ=255)
                                                                              aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACCAGcc                                                                                >  2:236380/1‑99 (MQ=255)
                                                                              aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGcc                                                                                <  1:236380/99‑1 (MQ=255)
                                                                              aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                         <  1:320357/106‑1 (MQ=255)
                                                                              aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                        <  2:307678/139‑1 (MQ=255)
                                                                                aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGCGGCGAGCGATAACCCAGCGCa                                      <  1:932789/139‑1 (MQ=255)
                                                                                aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCAc                                     >  2:1259699/1‑139 (MQ=255)
                                                                                  aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTcc                                                                                                                 <  1:16636/62‑1 (MQ=255)
                                                                                  aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACtt                                                                                               >  2:50994/1‑80 (MQ=255)
                                                                                  aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACt                                    <  2:765291/139‑1 (MQ=255)
                                                                                  aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACt                                    <  2:1060667/139‑1 (MQ=255)
                                                                                  aCAACTCCTTGGCAGGCCCTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt                                                                                                      <  2:477902/73‑1 (MQ=255)
                                                                                   cAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           <  1:1002066/83‑1 (MQ=255)
                                                                                   cAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                           >  2:1002066/1‑83 (MQ=255)
                                                                                    aaCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGTATTTGCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATg                                 >  1:671111/1‑139 (MQ=255)
                                                                                     aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            <  2:81804/64‑1 (MQ=255)
                                                                                     aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                     >  1:199442/1‑71 (MQ=255)
                                                                                     aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                     <  2:199442/71‑1 (MQ=255)
                                                                                      cTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                <  1:148156/59‑1 (MQ=255)
                                                                                      cTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGc                                >  2:1289104/1‑139 (MQ=255)
                                                                                       tCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATTACTTAAcc                                                                                           >  2:1145101/1‑79 (MQ=255)
                                                                                       tCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATTACTTAAcc                                                                                           <  1:1145101/79‑1 (MQ=255)
                                                                                          ttGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            >  1:397790/1‑59 (MQ=255)
                                                                                          ttGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            <  2:397790/59‑1 (MQ=255)
                                                                                           tGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAACGCACTATGCGGATg                           <  2:912048/139‑1 (MQ=255)
                                                                                             gCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACCTCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                         <  1:716250/139‑1 (MQ=255)
                                                                                             gCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                         <  1:771252/139‑1 (MQ=255)
                                                                                               aGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAt                       <  2:1178131/139‑1 (MQ=255)
                                                                                                 gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                >  2:1319704/1‑48 (MQ=255)
                                                                                                 gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt                                                                                                      <  1:585223/58‑1 (MQ=255)
                                                                                                 gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt                                                                                                      >  2:585223/1‑58 (MQ=255)
                                                                                                 gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                        >  1:78867/1‑88 (MQ=255)
                                                                                                 gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgc                                                        <  1:1253978/104‑1 (MQ=255)
                                                                                                 gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATg                           >  2:1008632/1‑133 (MQ=255)
                                                                                                 gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                         >  2:629322/1‑135 (MQ=255)
                                                                                                 gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                         <  1:629322/135‑1 (MQ=255)
                                                                                                 gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                      >  2:1298586/1‑138 (MQ=255)
                                                                                                  ccGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                           >  2:773060/1‑52 (MQ=255)
                                                                                                   cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                <  1:487087/110‑1 (MQ=255)
                                                                                                   cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                        >  2:839643/1‑118 (MQ=255)
                                                                                                     tAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcgagcga                                                  <  2:642804/106‑1 (MQ=255)
                                                                                                        aCAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATaa              <  2:1308479/139‑1 (MQ=255)
                                                                                                           aaTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                         <  2:546244/125‑1 (MQ=255)
                                                                                                            aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                            <  1:126256/41‑1 (MQ=255)
                                                                                                            aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                >  2:563152/1‑53 (MQ=255)
                                                                                                            aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGc                          <  2:724979/123‑1 (MQ=255)
                                                                                                             tCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTc         >  2:359595/1‑139 (MQ=255)
                                                                                                               cGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGa       >  2:1237632/1‑139 (MQ=255)
                                                                                                                gCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa      <  2:82677/139‑1 (MQ=38)
                                                                                                                   ttCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCg                                                            >  1:1325987/1‑82 (MQ=255)
                                                                                                                   ttCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGc   <  1:820247/139‑1 (MQ=25)
                                                                                                                    tCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg                                                         <  2:715971/84‑1 (MQ=255)
                                                                                                                    tCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATg                           >  1:868712/1‑114 (MQ=25)
                                                                                                                    tCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGcc  >  1:1078985/1‑139 (MQ=21)
                                                                                                                       tGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                >  1:1023698/1‑42 (MQ=255)
                                                                                                                       tGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                <  2:1023698/42‑1 (MQ=255)
                                                                                                                                      |                                                                                                                        
ATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCC  >  NC_000913/2068818‑2069072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: