Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	184,257	C→T	*271* (TAG→TAA)	yaeI ←	phosphodiesterase with model substrate bis‑pNPP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	184,257	0	C	T	100.0%	154.7 / NA	49	*271* (TAG→TAA)	yaeI	phosphodiesterase with model substrate bis‑pNPP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/27);  total (22/27)

TGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGAC  >  NC_000913/184124‑184391                                                                                                                                      |                                                                                                                                       tgtgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac                                                                                                                                   <  2:677465/139‑1 (MQ=255)     cTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGt                                                                                                                               >  2:1192793/1‑139 (MQ=255)        aTTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTTCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTcc                                                                                                                            <  2:816567/139‑1 (MQ=255)                               cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc                                                                                                                         >  2:859994/1‑119 (MQ=255)                               cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc                                                                                                                         <  1:859994/119‑1 (MQ=255)                                          tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCa                                                                                          <  2:303395/139‑1 (MQ=255)                                               tCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCa                                                                                     <  2:1307171/139‑1 (MQ=255)                                                   aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc                                                                                                                         >  1:1138464/1‑99 (MQ=255)                                                   aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc                                                                                                                         <  2:1138464/99‑1 (MQ=255)                                                      aaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac                                                                                                                                   <  2:392523/86‑1 (MQ=255)                                                      aaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac                                                                                                                                   >  1:392523/1‑86 (MQ=255)                                                      aaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACAcc                                                                                                                                  >  1:1250901/1‑87 (MQ=255)                                                      aaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACAcc                                                                                                                                  <  2:1250901/87‑1 (MQ=255)                                                      aaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc                                                                                                                         >  2:162428/1‑96 (MQ=255)                                                      aaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc                                                                                                                         <  1:162428/96‑1 (MQ=255)                                                            ttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCg                                                                                                            >  2:1152820/1‑103 (MQ=255)                                                            ttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCg                                                                                                            <  1:1152820/103‑1 (MQ=255)                                                            ttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCcca                                                                        <  1:284479/139‑1 (MQ=255)                                                                  ttacgccTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                  <  1:934993/133‑1 (MQ=255)                                                                           cTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                               >  2:976578/1‑133 (MQ=255)                                                                           cTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                               <  1:976578/133‑1 (MQ=255)                                                                                 tGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtgt                                                   >  2:1127654/1‑139 (MQ=255)                                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                       >  1:1167007/1‑85 (MQ=255)                                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                       <  2:1167007/85‑1 (MQ=255)                                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATgtgt                                                 >  1:925512/1‑139 (MQ=255)                                                                                   gCGTAAAAAGCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATgtgt                                                 >  1:902679/1‑139 (MQ=255)                                                                                    cGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCa                                                                                                                        >  1:1047911/1‑67 (MQ=255)                                                                                    cGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCa                                                                                                                        <  2:1047911/67‑1 (MQ=255)                                                                                     gTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCt                                               <  2:925512/139‑1 (MQ=255)                                                                                      tAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                         >  2:154745/1‑96 (MQ=255)                                                                                      tAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                         <  1:154745/96‑1 (MQ=255)                                                                                        aaaaTCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                       <  1:939461/80‑1 (MQ=255)                                                                                        aaaaTCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                       >  2:939461/1‑80 (MQ=255)                                                                                             cAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                  <  1:625931/112‑1 (MQ=255)                                                                                             cAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                  >  2:625931/1‑112 (MQ=255)                                                                                                                 tCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACg                   <  1:837041/139‑1 (MQ=255)                                                                                                                  cTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                               <  2:593019/94‑1 (MQ=255)                                                                                                                  cTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                               >  1:593019/1‑94 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCGAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                         <  2:261373/65‑1 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                       <  1:259228/51‑1 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                       >  2:259228/1‑51 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGtt                                                                                                  <  2:28079/56‑1 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGtt                                                                                                  >  1:28079/1‑56 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                         >  1:261373/1‑65 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc                                                                          <  2:396124/80‑1 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc                                                                          >  1:396124/1‑80 (MQ=255)                                                                                                                         tAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGttt           <  2:902679/139‑1 (MQ=255)                                                                                                                               tCACGATTTACACCAGTTCCAGCATCGTTACTTCCTGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTACATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTc     >  2:515033/1‑139 (MQ=255)                                                                                                                                  cGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGAc  <  1:1192793/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGAC  >  NC_000913/184124‑184391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑