Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,115,907	C→T	*407* (TAG→TAA)	wcaL ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,115,907	0	C	T	100.0%	97.0 / NA	31	*407* (TAG→TAA)	wcaL	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

TGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCG  >  NC_000913/2115776‑2116041                                                                                                                                    |                                                                                                                                       tGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAg                                                                                                                                 >  2:188622/1‑139 (MQ=255)                 aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 <  1:188622/139‑1 (MQ=255)                           cgggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGa                                                                                                       <  2:237271/139‑1 (MQ=255)                                      cgcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            <  1:467887/139‑1 (MQ=255)                                                       tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                         >  2:439286/1‑109 (MQ=255)                                                       tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                         <  1:439286/109‑1 (MQ=255)                                                           gaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGtt                                                                           <  2:243311/135‑1 (MQ=255)                                                           gaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGtt                                                                           >  1:243311/1‑135 (MQ=255)                                                            aggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTgca                                                                                                                           >  1:1231778/1‑86 (MQ=255)                                                            aggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTgca                                                                                                                           <  2:1231778/86‑1 (MQ=255)                                                              gaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTc                                                                          <  1:1207781/133‑1 (MQ=255)                                                              gaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTc                                                                          >  2:1207781/1‑133 (MQ=255)                                                               aaGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACtttt                                                                   >  2:1053635/1‑139 (MQ=255)                                                                   cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGAt                                                                                                  <  2:1171818/104‑1 (MQ=255)                                                                   cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGAt                                                                                                  >  1:1171818/1‑104 (MQ=255)                                                                            aGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGtt                                                      >  2:568531/1‑139 (MQ=255)                                                                                              tGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacgac                                               <  1:1259145/128‑1 (MQ=255)                                                                                              tGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacgac                                               >  2:1259145/1‑128 (MQ=255)                                                                                                cGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATtcg                                  >  2:494389/1‑139 (MQ=255)                                                                                                cGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGAGCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATtcg                                  >  1:899304/1‑139 (MQ=255)                                                                                                         agTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            >  2:1285317/1‑72 (MQ=255)                                                                                                         agTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            <  1:1285317/72‑1 (MQ=255)                                                                                                             ttttAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 <  2:1334478/47‑1 (MQ=255)                                                                                                             ttttAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 >  1:1334478/1‑47 (MQ=255)                                                                                                                tAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGc                  >  1:588064/1‑139 (MQ=255)                                                                                                                   aTGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTaa               >  2:1278209/1‑139 (MQ=255)                                                                                                                     ggCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAAc             >  2:832812/1‑139 (MQ=255)                                                                                                                       cATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGtt                                                                                                            >  2:357915/1‑42 (MQ=255)                                                                                                                       cATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGtt                                                                                                            <  1:357915/42‑1 (MQ=255)                                                                                                                         tGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 >  2:547105/1‑35 (MQ=255)                                                                                                                         tGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 <  1:547105/35‑1 (MQ=255)                                                                                                                                tccttTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAg                       >  1:139345/1‑118 (MQ=255)                                                                                                                                tccttTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAg                       <  2:139345/118‑1 (MQ=255)                                                                                                                                tccttTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGt    >  2:280970/1‑137 (MQ=255)                                                                                                                                tccttTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGt    <  1:280970/137‑1 (MQ=255)                                                                                                                                tccttTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCg  <  1:494389/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       TGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCG  >  NC_000913/2115776‑2116041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑