Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,130,853	C→T	S9N (AGC→AAC)  *406* (TAG→TAA)	wcaD ← wcaC ←	putative colanic acid polymerase putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,130,853	0	C	T	100.0%	123.9 / NA	39	S9N (AGC→AAC)  *406* (TAG→TAA)	wcaD wcaC	putative colanic acid polymerase putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/21);  total (18/21)

TTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAA  >  NC_000913/2130719‑2130981                                                                                                                                       |                                                                                                                                 ttttAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACa                                                                                                                              <  1:31196/139‑1 (MQ=255)    tAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAt                                                                                                                           >  2:550073/1‑139 (MQ=255)             tGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                                  <  2:765309/139‑1 (MQ=255)                      aaaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATa                                                                                                                    <  1:1064576/128‑1 (MQ=255)                      aaaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATa                                                                                                                    >  2:1064576/1‑128 (MQ=255)                       aaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                        <  1:332057/139‑1 (MQ=255)                            gcagTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAg                                                                                                                <  2:404620/126‑1 (MQ=255)                            gcagTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAg                                                                                                                >  1:404620/1‑126 (MQ=255)                              agTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACtcctcc                                                                                                 <  1:679854/139‑1 (MQ=255)                                   cAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCAt                                                                                            <  1:893506/139‑1 (MQ=255)                                     aGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACa                                                                                                                              <  2:703316/103‑1 (MQ=255)                                     aGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACa                                                                                                                              >  1:703316/1‑103 (MQ=255)                                      gACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtg                                                                                         <  1:589931/139‑1 (MQ=255)                                       aCAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                                  >  1:1211327/1‑113 (MQ=255)                                       aCAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                                  <  2:1211327/113‑1 (MQ=255)                                          ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAg                                                                                                                >  2:1192538/1‑112 (MQ=255)                                          ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAg                                                                                                                <  1:1192538/112‑1 (MQ=255)                                          ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActcct                                                                                                   >  1:1223316/1‑125 (MQ=255)                                          ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActcct                                                                                                   <  2:1223316/125‑1 (MQ=255)                                          ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                                     >  2:1306719/1‑139 (MQ=255)                                           gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                        >  2:1306303/1‑119 (MQ=255)                                           gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                        <  1:1306303/119‑1 (MQ=255)                                            tAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTcc                                                                                   >  2:952047/1‑139 (MQ=255)                                               aaaaGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACt                                                                                <  1:550073/139‑1 (MQ=255)                                                            gTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGgcggcgcgg                                                                   <  1:952047/139‑1 (MQ=255)                                                            gTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGgcggcgcgg                                                                   >  1:1085152/1‑139 (MQ=255)                                                             tGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGgcggcgcggc                                                                  >  1:350440/1‑139 (MQ=255)                                                                  gggAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGt                                                             >  2:1282525/1‑139 (MQ=255)                                                                                  gCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTcagcca                                             <  2:1085152/139‑1 (MQ=255)                                                                                  gCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTcagcca                                             <  1:1282525/139‑1 (MQ=255)                                                                                                     aaCCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGCAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTAc                                    <  2:824606/129‑1 (MQ=255)                                                                                                     aaCCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCg                                                              >  2:1058276/1‑103 (MQ=255)                                                                                                     aaCCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCg                                                              <  1:1058276/103‑1 (MQ=255)                                                                                                     aaCCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGCTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTCGTAc                                    >  1:824606/1‑129 (MQ=255)                                                                                                      aCCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGc                                                         <  2:145108/107‑1 (MQ=255)                                                                                                      aCCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGc                                                         >  1:145108/1‑107 (MQ=255)                                                                                                                            gcaACAGGTAGTTACAGATTCTGATAGAAGTTGACATActc                                                                                                     <  1:473234/41‑1 (MQ=255)                                                                                                                            gcaACAGGTAGTTACAGATTCTGATAGAAGTTGACATActc                                                                                                     >  2:473234/1‑41 (MQ=255)                                                                                                                             caACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTaa  >  1:246520/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                 TTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAA  >  NC_000913/2130719‑2130981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑