Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,164,998	G→A	*241* (TAG→TAA)	baeR →	response regulator in two‑component regulatory system with BaeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,164,998	0	G	A	100.0%	103.7 / NA	33	*241* (TAG→TAA)	baeR	response regulator in two‑component regulatory system with BaeS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/17);  total (16/17)

CGACCGCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATT  >  NC_000913/2164866‑2165085                                                                                                                                     |                                                                                        cgaccgCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAg                                                                                   <  2:182001/139‑1 (MQ=255) cgaccgCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAg                                                                                   <  1:280979/139‑1 (MQ=255)  gaccgCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGc                                                                                  >  1:1096344/1‑139 (MQ=255)     cgCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGAc                                                                               >  1:630888/1‑139 (MQ=255)       cACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACat                                                                             >  2:1025953/1‑139 (MQ=255)               aCAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTGGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGt                                                                     >  1:660702/1‑139 (MQ=255)                  gCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAg                                                                                   <  1:1207382/122‑1 (MQ=255)                  gCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAg                                                                                   >  2:1207382/1‑122 (MQ=255)                          aaaaaCCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTGGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGAt                                                          <  2:660702/139‑1 (MQ=255)                                 tGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTc                                                   >  2:639993/1‑139 (MQ=255)                                   cgccgcAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAg                                                 >  1:254043/1‑139 (MQ=255)                                    gccgcAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCTGa                                                <  1:639993/139‑1 (MQ=255)                                        cAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGa                                                <  1:771884/135‑1 (MQ=255)                                        cAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGACGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGa                                                >  2:771884/1‑135 (MQ=255)                                         aaGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGa                                           <  1:586368/139‑1 (MQ=255)                                              ggAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCgg                                      <  2:254043/139‑1 (MQ=255)                                                aaTCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCa                                    >  2:1166051/1‑139 (MQ=255)                                                    tctcGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACAt                                <  1:1166051/139‑1 (MQ=255)                                                         aCGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGAc                                                                               >  1:216782/1‑87 (MQ=255)                                                         aCGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGAc                                                                               <  2:216782/87‑1 (MQ=255)                                                                    tCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGa                                                                                >  2:257229/1‑75 (MQ=255)                                                                    tCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGa                                                                                <  1:257229/75‑1 (MQ=255)                                                                         tATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATc                            <  1:444907/122‑1 (MQ=255)                                                                         tATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATc                            >  2:444907/1‑122 (MQ=255)                                                                          aTCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCaaa          <  1:614174/139‑1 (MQ=255)                                                                                            gTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCgg                                      >  2:1210560/1‑93 (MQ=255)                                                                                            gTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCgg                                      <  1:1210560/93‑1 (MQ=255)                                                                                               ggTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGtt                                                    <  2:1031910/76‑1 (MQ=255)                                                                                               ggTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGtt                                                    >  1:1031910/1‑76 (MQ=255)                                                                                                                  aCGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTc                <  2:1113592/93‑1 (MQ=255)                                                                                                                  aCGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTc                >  1:1113592/1‑93 (MQ=255)                                                                                                                             aTCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACAtt  >  2:1342873/1‑96 (MQ=255)                                                                                                                             aTCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACAtt  <  1:1342873/96‑1 (MQ=255)                                                                                                                                     |                                                                                        CGACCGCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATT  >  NC_000913/2164866‑2165085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑