Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,189,594	C→T	A439A (GCG→GCA)	yehB ←	putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,189,594	0	C	T	100.0%	188.3 / NA	59	A439A (GCG→GCA)	yehB	putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/30);  total (29/30)

TAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAT  >  NC_000913/2189499‑2189725                                                                                                |                                                                                                                                    taaaCATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTgg                                                                                                          <  1:459785/120‑1 (MQ=255) tAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTgg                                                                                                          >  2:459785/1‑123 (MQ=255)      aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCa                                                                                     >  2:1003625/1‑139 (MQ=255)      aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCa                                                                                     >  1:417949/1‑139 (MQ=255)        cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCt                                                                                                        >  2:980389/1‑118 (MQ=255)        cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCt                                                                                                        <  1:980389/118‑1 (MQ=255)        cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                   >  2:536044/1‑139 (MQ=255)        cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                   >  1:119219/1‑139 (MQ=255)        cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                   >  2:452115/1‑139 (MQ=255)               atcaCGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATaa                                                                            >  2:17308/1‑139 (MQ=255)                    ggCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTg                                                                       >  2:232944/1‑139 (MQ=255)                      cGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGcc                                                                     <  1:17308/139‑1 (MQ=255)                            ttATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCaa                                                               <  1:536044/139‑1 (MQ=255)                                  ttATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACAt                                                         <  2:319071/139‑1 (MQ=255)                                     ttGTTTGCCCAAACGTGATCGTTAAATGTCCAGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGc                                                      >  2:518839/1‑139 (MQ=255)                                      tGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGcc                                                     >  2:8183/1‑139 (MQ=255)                                         ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  <  2:417949/139‑1 (MQ=255)                                         ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  <  1:1256747/139‑1 (MQ=255)                                          tGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGATGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  <  2:847752/138‑1 (MQ=255)                                          tGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGc                                                                      <  1:495557/118‑1 (MQ=255)                                          tGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGc                                                                      >  2:495557/1‑118 (MQ=255)                                          tGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  >  1:847752/1‑138 (MQ=255)                                              cAAACGTGATCGTTAAATGTCCAGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCtt                                             <  1:518839/139‑1 (MQ=255)                                                      ctCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTa                                     >  2:471062/2‑139 (MQ=255)                                                      aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTca                                                                                                      <  2:1067170/74‑1 (MQ=255)                                                      aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTca                                                                                                      >  1:1067170/1‑74 (MQ=255)                                                      aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCaa                                                                                    >  1:698136/1‑92 (MQ=255)                                                      aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCaa                                                                                    <  2:698136/92‑1 (MQ=255)                                                      aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTa                                     >  2:1001688/1‑139 (MQ=255)                                                       tCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTAt                                    <  1:8183/139‑1 (MQ=255)                                                        cGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATg                                   <  1:232944/139‑1 (MQ=255)                                                         gTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  >  2:503773/1‑123 (MQ=255)                                                         gTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  <  1:503773/123‑1 (MQ=255)                                                           tAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGa                                >  1:382137/1‑139 (MQ=255)                                                           tAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGa                                <  1:452115/139‑1 (MQ=255)                                                                  ccGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATg                                   >  1:1039092/1‑129 (MQ=255)                                                                  ccGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATg                                   <  2:1039092/129‑1 (MQ=255)                                                                   cGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  >  1:586736/1‑113 (MQ=255)                                                                   cGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  <  2:586736/113‑1 (MQ=255)                                                                    ggTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCt                                                                                                        >  1:528852/1‑58 (MQ=255)                                                                    ggTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCt                                                                                                        <  2:528852/58‑1 (MQ=255)                                                                    ggTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGc                       >  1:277719/1‑139 (MQ=255)                                                                     gTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGt                                                <  2:208315/113‑1 (MQ=255)                                                                     gTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGt                                                >  1:208315/1‑113 (MQ=255)                                                                     gTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCa                      <  2:277719/139‑1 (MQ=255)                                                                     gTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCa                      >  2:826830/1‑139 (MQ=255)                                                                           aCGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACg                <  2:119219/139‑1 (MQ=255)                                                                           aCGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACg                >  1:1132077/1‑139 (MQ=255)                                                                               gacgaATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTa                            >  2:505427/1‑123 (MQ=255)                                                                               gacgaATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTa                            <  1:505427/123‑1 (MQ=255)                                                                                acgaATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGt                                                <  2:203063/102‑1 (MQ=255)                                                                                acgaATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGt                                                >  1:203063/1‑102 (MQ=255)                                                                                 cgaATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATg          <  1:471062/139‑1 (MQ=255)                                                                                        cGCCAGGCTGCCAGACAAAAACGCGTCGACGTTTGGCTGACAAATTTGTTGGAGGAAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCaa   <  2:36962/139‑1 (MQ=255)                                                                                         gCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAt  <  2:1132077/139‑1 (MQ=255)                                                                                         gCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAt  <  2:690982/139‑1 (MQ=255)                                                                                         gCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAt  <  2:382137/139‑1 (MQ=255)                                                                                             ggCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATg                                   >  1:575671/1‑102 (MQ=255)                                                                                             ggCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATg                                   <  2:575671/102‑1 (MQ=255)                                                                                                |                                                                                                                                    TAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAT  >  NC_000913/2189499‑2189725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑