Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 200,214 | C→T | P763S (CCA→TCA) | bamA → | BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 200,214 | 0 | C | T | 100.0% | 190.5 / NA | 59 | P763S (CCA→TCA) | bamA | BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor |
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/29); total (30/29) |
CATCACCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTC > NC_000913/200081‑200333 | catcaCCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGc > 2:399734/1‑139 (MQ=255) tttATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTg > 1:1224312/1‑139 (MQ=255) cGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCt < 1:631777/130‑1 (MQ=255) cGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCt > 2:631777/1‑130 (MQ=255) aTAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat < 1:88441/116‑1 (MQ=255) aTAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat > 2:88441/1‑116 (MQ=255) tGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa < 2:324927/107‑1 (MQ=255) tGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa > 1:324927/1‑107 (MQ=255) tGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg < 2:305455/126‑1 (MQ=255) tGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg > 1:305455/1‑126 (MQ=255) tGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa > 1:1054488/1‑133 (MQ=255) tGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa < 2:1054488/133‑1 (MQ=255) cTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAatat < 2:1168919/109‑1 (MQ=255) cTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAatat > 1:1168919/1‑109 (MQ=255) tAACTCGGTTCGTACTTCATTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATgg < 1:905999/139‑1 (MQ=255) cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGt > 2:607901/1‑109 (MQ=255) cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGt < 1:607901/109‑1 (MQ=255) cTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATg > 2:826778/1‑139 (MQ=255) cGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTc > 2:517250/1‑139 (MQ=255) ttCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa > 2:663352/1‑96 (MQ=255) ttCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa < 1:663352/96‑1 (MQ=255) ttCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTcccc > 1:481708/1‑139 (MQ=255) tACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTg > 1:117828/1‑139 (MQ=255) ttcttcTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGt < 2:804476/139‑1 (MQ=255) cttctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTg < 1:517250/139‑1 (MQ=255) cttctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTg < 2:117828/139‑1 (MQ=255) ttctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTg < 1:993288/138‑1 (MQ=255) ttctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTg > 2:993288/1‑138 (MQ=255) gggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGtt < 2:481708/139‑1 (MQ=255) atatGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTtctc < 1:826778/139‑1 (MQ=255) tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTa > 1:20800/1‑93 (MQ=255) tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTa < 2:20800/93‑1 (MQ=255) tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTa > 2:75026/1‑139 (MQ=255) gggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTAc > 1:675132/1‑139 (MQ=255) tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTAt < 2:756942/85‑1 (MQ=255) tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTAt > 1:756942/1‑85 (MQ=255) tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa > 1:1095487/1‑89 (MQ=255) tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa < 2:1095487/89‑1 (MQ=255) tACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCaaa < 1:75026/139‑1 (MQ=255) cAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa > 2:424952/1‑86 (MQ=255) cAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa < 1:424952/86‑1 (MQ=255) tGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACg > 1:1042257/1‑139 (MQ=255) gggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGa > 2:680153/1‑139 (MQ=255) tCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtgt < 1:691605/103‑1 (MQ=255) tCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtgt > 2:691605/1‑103 (MQ=255) ccAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGgaga < 1:865181/139‑1 (MQ=255) tatTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAg > 1:839770/1‑139 (MQ=255) tatTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAg > 1:51495/1‑139 (MQ=255) tGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCaaa > 1:177319/1‑112 (MQ=255) tGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCaaa < 2:177319/112‑1 (MQ=255) ggATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGt < 2:675132/139‑1 (MQ=255) gATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGtt > 1:1198645/1‑139 (MQ=255) atatCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGttt < 2:839770/139‑2 (MQ=255) atatCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTc < 2:51495/139‑1 (MQ=255) atatCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTc < 2:1198645/139‑1 (MQ=255) ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTccc > 2:703159/1‑64 (MQ=255) ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTccc < 1:703159/64‑1 (MQ=255) gACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGtt < 1:374636/80‑1 (MQ=255) gACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGtt > 2:374636/1‑80 (MQ=255) | CATCACCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTC > NC_000913/200081‑200333 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |