Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,233,597	G→A	*240* (TAG→TAA)	sanA →	DUF218 superfamily vancomycin high temperature exclusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,233,597	0	G	A	100.0%	116.0 / NA	37	*240* (TAG→TAA)	sanA	DUF218 superfamily vancomycin high temperature exclusion protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

TTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAC  >  NC_000913/2233463‑2233723                                                                                                                                       |                                                                                                                               tttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTAt                                                                                                                            <  1:990256/139‑1 (MQ=255) tttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTAt                                                                                                                            <  2:608276/139‑1 (MQ=255)                               cTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGt                                                                                              >  1:864562/1‑139 (MQ=255)                               cTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGt                                                                                              >  1:558766/1‑139 (MQ=255)                               cTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCATCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGt                                                                                              >  2:42524/1‑139 (MQ=255)                                       tATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATTCCGCCGTCACACCTGAACACATAGTTGAATTACAACAGAAACACGGACAGCAATTATGCTTGTACACAAATTTTATGTCGTTGCCGTTTTTTTcc                                                                                      >  2:811079/1‑139 (MQ=255)                                          tCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTa                                                                                   <  1:277415/139‑1 (MQ=255)                                            cGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCATCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATc                                                                                 <  1:42524/139‑1 (MQ=255)                                              gCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCt                                                                                     <  2:654382/133‑1 (MQ=255)                                              gCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCt                                                                                     >  1:654382/1‑133 (MQ=255)                                              gCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATccc                                                                               >  1:353675/1‑139 (MQ=255)                                                 aTGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATg                                                                                                                       <  2:118784/96‑1 (MQ=255)                                                 aTGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATg                                                                                                                       >  1:118784/1‑96 (MQ=255)                                                            aCCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAGCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTcc                                                                 >  2:876503/1‑139 (MQ=255)                                                            aCCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTcc                                                                 >  1:3088/1‑139 (MQ=255)                                                             ccGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcg                                                                <  2:353675/139‑1 (MQ=255)                                                                  aGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAg                                                           <  2:558766/139‑1 (MQ=255)                                                                  aGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAg                                                           <  2:3088/139‑1 (MQ=255)                                                                            gggTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAgg                                                 <  2:1172814/139‑1 (MQ=255)                                                                               tATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTg                                              <  1:1097621/139‑1 (MQ=255)                                                                                      ccgTCACCCCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATAttt                                                                        >  1:823056/1‑106 (MQ=255)                                                                                      ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATAttt                                                                        <  2:823056/106‑1 (MQ=255)                                                                                      ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAAGAAAGTAATAATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCa                                       >  2:830056/1‑139 (MQ=255)                                                                                           acacCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGc                                                                                                 <  2:456/76‑1 (MQ=255)                                                                                           acacCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGc                                                                                                 >  1:456/1‑76 (MQ=255)                                                                                               ccGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGgcgcg                                         >  1:797313/1‑128 (MQ=255)                                                                                               ccGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGAATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGgcgcg                                         <  2:797313/128‑1 (MQ=255)                                                                                                 gAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGa                            >  2:851091/1‑139 (MQ=255)                                                                                                 gAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGa                            >  1:667507/1‑139 (MQ=255)                                                                                                         aCTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAcag                                                                                                                  <  2:195023/45‑1 (MQ=255)                                                                                                         aCTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAcag                                                                                                                  >  1:195023/1‑45 (MQ=255)                                                                                                         aCTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCg                                                                                               <  2:1202316/64‑1 (MQ=255)                                                                                                         aCTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCg                                                                                               >  1:1202316/1‑64 (MQ=255)                                                                                                                 tACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCTCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAAt            >  1:155832/1‑139 (MQ=255)                                                                                                                     aaaGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGc                                                                                                 <  1:878057/50‑1 (MQ=255)                                                                                                                     aaaGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGc                                                                                                 >  2:878057/1‑50 (MQ=255)                                                                                                                           aCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc  <  2:155832/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                               TTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAC  >  NC_000913/2233463‑2233723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑