Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,742	T→C	L192P (CTG→CCG)	dnaJ →	chaperone Hsp40, DnaK co‑chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,742	0	T	C	100.0%	204.3 / NA	57	L192P (CTG→CCG)	dnaJ	chaperone Hsp40, DnaK co‑chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/25);  total (32/25)

TAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCG  >  NC_000913/14614‑14873                                                                                                                                 |                                                                                                                                    tAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGAt                                                                                                                           <  1:58258/139‑1 (MQ=255)       tGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGc                                                                                                                     <  1:21847/139‑1 (MQ=255)                 ggTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATgtc                                                                                                           <  1:597799/139‑1 (MQ=255)                 ggTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATgtc                                                                                                           >  2:597799/1‑139 (MQ=255)                      acaGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATgtcatggt                                                                                                      <  2:863576/139‑1 (MQ=255)                                    gTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGa                                                                                        >  1:218656/1‑139 (MQ=255)                                            tGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCgcagca                                                                                >  2:1018663/1‑139 (MQ=255)                                            tGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCgcagca                                                                                <  1:629012/139‑1 (MQ=255)                                                      cTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTc                                                                      <  1:1018663/139‑1 (MQ=255)                                                        ggTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCg                                                                    >  2:1041044/1‑139 (MQ=255)                                                        ggTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCg                                                                    >  2:1124976/1‑139 (MQ=255)                                                          tCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGtt                                                                  >  1:506483/1‑139 (MQ=255)                                                              gTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCGAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTaaaa                                                              >  1:242513/1‑139 (MQ=255)                                                              gTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGtcatggtcatgg                                                                                                 <  1:572354/104‑1 (MQ=255)                                                              gTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGtcatggtcatgg                                                                                                 >  2:572354/1‑104 (MQ=255)                                                              gTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTaaaa                                                              >  2:383289/1‑139 (MQ=255)                                                              gTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTaaaa                                                              >  1:117499/1‑139 (MQ=255)                                                              gTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTaaaa                                                              >  2:1160476/1‑139 (MQ=255)                                                              gTGCAGATGCGCCAGGGATTCTTCACTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTaaaa                                                              >  1:572329/1‑139 (MQ=255)                                                               tGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCGAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAAt                                                             <  2:242513/139‑1 (MQ=255)                                                                   gATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCg                                                         >  1:738059/1‑139 (MQ=255)                                                                    aTGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCgg                                                        <  1:1160476/139‑1 (MQ=255)                                                                        gcCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGcaaca                                                                                                                 >  2:1076864/1‑78 (MQ=255)                                                                        gcCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGcaaca                                                                                                                 <  1:1076864/78‑1 (MQ=255)                                                                              gATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGa                                              <  1:1041044/139‑1 (MQ=255)                                                                                  cttcGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCACAGATCCGTGCAACAAATGTCATGGTCATGTTCGTGtt                                                                                          >  1:1309137/1‑91 (MQ=255)                                                                                  cttcGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGtt                                                                                          <  2:1309137/91‑1 (MQ=255)                                                                                   ttcGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTg                                         <  2:506483/139‑1 (MQ=255)                                                                                    tcGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGcaaca                                                                                                                 <  1:613698/66‑1 (MQ=255)                                                                                    tcGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGcaaca                                                                                                                 >  2:613698/1‑66 (MQ=255)                                                                                        tGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGAc                                    >  2:1286927/1‑139 (MQ=255)                                                                                        tGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGAc                                    <  1:383289/139‑1 (MQ=255)                                                                                            cagcagACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCa                                >  1:1050018/1‑139 (MQ=255)                                                                                               cagACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATcc                             >  1:865450/1‑139 (MQ=255)                                                                                               cagACCTGTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATcc                             >  2:475954/1‑139 (MQ=255)                                                                                                      gTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTcatggtcatggt                                                                                                >  1:534145/1‑65 (MQ=255)                                                                                                      gTCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTcatggtcatggt                                                                                                <  2:534145/65‑1 (MQ=255)                                                                                                       tCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCgg                                                        >  2:831666/1‑104 (MQ=255)                                                                                                       tCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCgg                                                        <  1:831666/104‑1 (MQ=255)                                                                                                       tCCACACTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCg                     >  2:1244118/1‑139 (MQ=255)                                                                                                         cacaCTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCggg                   <  2:117499/139‑1 (MQ=255)                                                                                                         cacaCTGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCggg                   <  2:572329/139‑1 (MQ=255)                                                                                                              tGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACaaa                                                                                                               >  1:822006/1‑42 (MQ=255)                                                                                                              tGTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACaaa                                                                                                               <  2:822006/42‑1 (MQ=255)                                                                                                               gTCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAgg             >  1:261337/1‑139 (MQ=255)                                                                                                                tCAGGGCCGCGGTATGCCGATCAAAGATCCGTGCAACAAATgtca                                                                                                          <  2:252791/45‑1 (MQ=255)                                                                                                                tCAGGGCCGCGGTATGCCGATCAAAGATCCGTGCAACAAATgtca                                                                                                          >  1:252791/1‑45 (MQ=255)                                                                                                                tCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGAc                                    <  2:879021/115‑1 (MQ=255)                                                                                                                tCAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGAc                                    >  1:879021/1‑115 (MQ=255)                                                                                                                 cAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAggg                                                   >  2:1288443/1‑99 (MQ=255)                                                                                                                 cAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAggg                                                   <  1:1288443/99‑1 (MQ=255)                                                                                                                 cAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAg              >  1:481052/1‑136 (MQ=255)                                                                                                                 cAGGGCCGCGGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAg              <  2:481052/136‑1 (MQ=255)                                                                                                                        gcgGTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCggg    >  1:1103444/1‑139 (MQ=255)                                                                                                                          ggTACGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCg  >  1:50849/1‑139 (MQ=255)                                                                                                                              cGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACg                                                                           >  1:1132858/1‑62 (MQ=255)                                                                                                                              cGCCGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACg                                                                           <  2:1132858/62‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    TAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCG  >  NC_000913/14614‑14873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑