Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,514,077	T→C	F346L (TTC→CTC)	nupC →	nucleoside (except guanosine) transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,514,077	0	T	C	100.0%	199.6 / NA	55	F346L (TTC→CTC)	nupC	nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/25);  total (30/25)

TCCAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTG  >  NC_000913/2513948‑2514205                                                                                                                                  |                                                                                                                                 tccAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGttt                                                                                                                         >  2:1147064/1‑139 (MQ=255) tccAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGttt                                                                                                                         >  1:108771/1‑139 (MQ=255)      tGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTc                                                                                                                                >  2:585979/1‑127 (MQ=255)        aaGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGc                                                                                                                  >  1:662547/1‑139 (MQ=255)            aCTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAAc                                                                                                              <  1:1270288/139‑1 (MQ=255)                  aGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTctct                                                                                                        <  2:662547/139‑1 (MQ=255)                       gCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAAt                                                                                                   <  1:1177611/139‑1 (MQ=255)                        cAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATc                                                                                                  >  1:1001602/1‑139 (MQ=255)                                     aCCAATCTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGc                                                                                       >  2:527835/1‑137 (MQ=255)                                     aCCAATCTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGc                                                                                       <  1:527835/137‑1 (MQ=255)                                     aCCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTc                                                                                                                                <  1:565269/96‑1 (MQ=255)                                     aCCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTc                                                                                                                                >  2:565269/1‑96 (MQ=255)                                     aCCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCt                                                                                                                 >  1:341998/1‑111 (MQ=255)                                     aCCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCt                                                                                                                 <  2:341998/111‑1 (MQ=255)                                     aCCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAg                                                                                     >  1:55756/1‑139 (MQ=255)                                     aCCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAg                                                                                     >  2:164089/1‑139 (MQ=255)                                        aaaCTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTg                                                                                  <  1:1147064/139‑1 (MQ=255)                                                    aaCGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGcc                                                                      <  2:1001602/139‑1 (MQ=255)                                                          ttCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAg                                                                                     <  1:1289284/118‑1 (MQ=255)                                                          ttCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAg                                                                                     >  2:1289284/1‑118 (MQ=255)                                                          ttCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCt                                                                     <  2:220392/134‑1 (MQ=255)                                                          ttCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGAAGGTGCAGTTAAAGGCCt                                                                     >  1:220392/1‑134 (MQ=255)                                                            cGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCtgaatgaa                                                              >  1:712883/1‑139 (MQ=255)                                                              ttGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCg                                                                                        <  2:346413/111‑1 (MQ=255)                                                              ttGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCg                                                                                        >  1:346413/1‑111 (MQ=255)                                                               tGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAagag                                                           <  2:108771/139‑1 (MQ=255)                                                                               cAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGttt                                           >  2:1332266/1‑139 (MQ=255)                                                                               cAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGttt                                           >  2:1243413/1‑139 (MQ=255)                                                                                          ttCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGt                                <  2:475564/139‑1 (MQ=255)                                                                                                   ctctcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCtga                                                                   >  1:1223483/1‑95 (MQ=255)                                                                                                   ctctcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCtga                                                                   <  2:1223483/95‑1 (MQ=255)                                                                                                        cgcgTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTa                  <  2:55756/139‑1 (MQ=255)                                                                                                           gTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACgg               >  2:1288139/1‑139 (MQ=255)                                                                                                            tGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTg                                                                                  >  2:202074/1‑71 (MQ=255)                                                                                                            tGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTg                                                                                  <  1:202074/71‑1 (MQ=255)                                                                                                             gCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct             <  2:712883/139‑1 (MQ=255)                                                                                                              cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGc                                                                                       <  2:847430/64‑1 (MQ=255)                                                                                                              cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGc                                                                                       >  1:847430/1‑64 (MQ=255)                                                                                                              cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGctc            >  2:1024750/1‑139 (MQ=255)                                                                                                              cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGctc            >  1:954081/1‑139 (MQ=255)                                                                                                              cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGctc            >  2:688872/1‑139 (MQ=255)                                                                                                                gAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCg                                                                                        >  2:1105138/1‑61 (MQ=255)                                                                                                                gAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCg                                                                                        <  1:1105138/61‑1 (MQ=255)                                                                                                                gAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCt                                                                     <  2:76154/80‑1 (MQ=255)                                                                                                                gAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCt                                                                     >  1:76154/1‑80 (MQ=255)                                                                                                                gAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTtc                                          >  2:919201/1‑107 (MQ=255)                                                                                                                gAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTtc                                          <  1:919201/107‑1 (MQ=255)                                                                                                                        catcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCa                                                                                      >  2:502302/1‑55 (MQ=255)                                                                                                                        catcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCa                                                                                      <  1:502302/55‑1 (MQ=255)                                                                                                                        catcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTg  >  1:1097658/1‑139 (MQ=255)                                                                                                                        catcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTg  <  1:1038471/139‑1 (MQ=255)                                                                                                                         atcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAagag                                                           >  2:1045726/1‑81 (MQ=255)                                                                                                                         atcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAagag                                                           <  1:1045726/81‑1 (MQ=255)                                                                                                                              tgtgCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTa                                                                            <  2:1044037/59‑1 (MQ=255)                                                                                                                              tgtgCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTa                                                                            >  1:1044037/1‑59 (MQ=255)                                                                                                                                  |                                                                                                                                 TCCAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTG  >  NC_000913/2513948‑2514205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑