Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,781,132	T→C	D532G (GAC→GGC)	ypjA ←	adhesin‑like autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,781,132	0	T	C	100.0%	199.1 / NA	56	D532G (GAC→GGC)	ypjA	adhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/30);  total (26/30)

CATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGTCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCA  >  NC_000913/2780998‑2781258                                                                                                                                       |                                                                                                                               cATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                            >  2:549700/1‑139 (MQ=255)    ttttAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAAc                                                                                                                         <  1:1044765/139‑1 (MQ=255)            tCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGaa                                                                                                                 <  2:460462/139‑1 (MQ=255)             cTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGa                                                                                                                           <  1:1032361/128‑1 (MQ=255)             cTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGa                                                                                                                           >  2:1032361/1‑128 (MQ=255)               gAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgc                                                                                                              <  2:1063499/139‑1 (MQ=255)                 aTTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                            <  1:1323022/139‑1 (MQ=255)                   tCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                          <  2:674804/139‑1 (MQ=255)                       gATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGaa                                                                                                                 <  2:457942/128‑1 (MQ=255)                       gATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGaa                                                                                                                 >  1:457942/1‑128 (MQ=255)                                   aTCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCattatt                                                                                                    <  2:298804/129‑1 (MQ=255)                                   aTCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCattatt                                                                                                    >  1:298804/1‑129 (MQ=255)                                   aTCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTg                                                                                          >  2:87711/1‑139 (MQ=255)                                   aTCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTg                                                                                          >  2:728632/1‑139 (MQ=255)                                       ccATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                      <  1:87711/139‑1 (MQ=255)                                            aCTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGtt                                                                                 >  1:618061/1‑139 (MQ=255)                                                ttGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCt                                                                                                                             >  1:1325336/1‑91 (MQ=255)                                                ttGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCt                                                                                                                             <  2:1325336/91‑1 (MQ=255)                                                ttGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAg                                                                             >  1:369242/1‑139 (MQ=255)                                                  ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                          >  1:676705/1‑108 (MQ=255)                                                  ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                          <  2:676705/108‑1 (MQ=255)                                                  ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCa                                                                                                         <  2:1241316/109‑1 (MQ=255)                                                  ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCa                                                                                                         >  1:1241316/1‑109 (MQ=255)                                                  ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                           <  2:430265/139‑1 (MQ=255)                                                                   ttCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCCGAACgccacc                                                                                                          >  1:1153760/1‑91 (MQ=255)                                                                   ttCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                          <  2:844949/91‑1 (MQ=255)                                                                   ttCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                          >  1:844949/1‑91 (MQ=255)                                                                   ttCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                          <  2:1153760/91‑1 (MQ=255)                                                                         aGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGa                                                                                                                           >  2:377449/1‑68 (MQ=255)                                                                         aGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGa                                                                                                                           <  1:377449/68‑1 (MQ=255)                                                                           gTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                            <  2:592485/65‑1 (MQ=255)                                                                           gTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                            >  1:592485/1‑65 (MQ=255)                                                                            tCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGcc                                                 <  1:549700/139‑1 (MQ=255)                                                                             cGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCa                                                <  1:728632/139‑1 (MQ=255)                                                                             cGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCa                                                >  1:617031/1‑139 (MQ=255)                                                                               tGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                            <  1:1293748/61‑1 (MQ=255)                                                                               tGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                            >  2:1293748/1‑61 (MQ=255)                                                                               tGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCatt                                                                                                       >  2:2283/1‑82 (MQ=255)                                                                               tGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCatt                                                                                                       <  1:2283/82‑1 (MQ=255)                                                                               tGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGt                                              <  1:231564/139‑1 (MQ=255)                                                                               tGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGt                                              >  2:231564/1‑139 (MQ=255)                                                                                  aaGCAAGTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATc                                           <  2:13620/139‑1 (MQ=255)                                                                                    gCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCaa                                         <  2:618061/139‑1 (MQ=255)                                                                                            tGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCtt                                                                      >  2:361709/1‑102 (MQ=255)                                                                                            tGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCtt                                                                      <  1:361709/102‑1 (MQ=255)                                                                                                 cgccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCattatt                                                                                                    <  1:741978/67‑1 (MQ=255)                                                                                                 cgccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCattatt                                                                                                    >  2:741978/1‑67 (MQ=255)                                                                                                  gccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTc                           >  1:1171079/1‑139 (MQ=255)                                                                                                   ccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCg                          >  2:1071354/1‑139 (MQ=255)                                                                                                      gATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCg                       <  2:369242/139‑1 (MQ=255)                                                                                                      gATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCg                       <  2:617031/139‑1 (MQ=255)                                                                                                           ttgCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTc                                                                                               >  2:1101046/1‑62 (MQ=255)                                                                                                           ttgCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTc                                                                                               <  1:1101046/62‑1 (MQ=255)                                                                                                                           gCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCa  >  2:1132081/1‑139 (MQ=255)                                                                                                                               caccacCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                      <  2:639188/51‑1 (MQ=255)                                                                                                                               caccacCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                      >  1:639188/1‑51 (MQ=255)                                                                                                                                       |                                                                                                                               CATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGTCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCA  >  NC_000913/2780998‑2781258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑