| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,856,453 | C→T | *118* (TAG→TAA) | ygbA ← | uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,856,453 | 0 | C | T | 94.4% | 99.2 / ‑1.5 | 36 | *118* (TAG→TAA) | ygbA | uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (17/17); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
TAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAA > NC_000913/2856326‑2856580 | tAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAgg > 2:348498/1‑139 (MQ=255) cTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGc < 2:568299/139‑1 (MQ=255) cACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGt < 2:447574/107‑1 (MQ=255) cACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGt > 1:447574/1‑107 (MQ=255) gctgTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACg < 1:296128/139‑1 (MQ=255) ctgTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGc > 2:32785/1‑139 (MQ=255) aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGa > 1:1091146/1‑101 (MQ=255) aCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGa < 2:1091146/101‑1 (MQ=255) ggATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCAt < 1:32785/139‑1 (MQ=255) gATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATc > 2:620829/1‑139 (MQ=255) aGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgat > 1:1037317/1‑137 (MQ=255) aGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgat < 2:1037317/137‑1 (MQ=255) gAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa < 2:392295/139‑1 (MQ=255) gAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCa < 1:348498/139‑1 (MQ=255) aTAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgat < 1:1283349/122‑1 (MQ=255) aTAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCgat > 2:1283349/1‑122 (MQ=255) aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATAtt > 2:1317440/1‑82 (MQ=255) aaTCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATAtt < 1:1317440/82‑1 (MQ=255) tCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGc > 1:1306198/1‑88 (MQ=255) tCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGc < 2:1306198/88‑1 (MQ=255) tCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTa < 2:821500/134‑1 (MQ=255) tCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTa > 1:821500/1‑134 (MQ=255) aCTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCACGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc < 2:1023991/113‑1 (MQ=255) aCTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCACGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc > 1:1023991/1‑113 (MQ=255) cAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg < 1:571320/108‑1 (MQ=255) cAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACg > 2:571320/1‑108 (MQ=255) cAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc > 2:509092/1‑109 (MQ=255) cAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGc < 1:509092/109‑1 (MQ=255) cAAATTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCAtt > 1:850785/1‑139 (MQ=255) aaaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCga > 2:1140220/1‑97 (MQ=255) aaaTTGCCGGACAGATCTGCCTGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCga < 1:1140220/97‑1 (MQ=255) tGTCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATaa > 2:67402/1‑139 (MQ=255) tCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCg < 1:540691/44‑1 (MQ=255) tCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCg > 2:540691/1‑44 (MQ=255) tCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt < 2:1046166/90‑1 (MQ=255) tCCGGCATATTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt > 1:1046166/1‑90 (MQ=255) | TAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAA > NC_000913/2856326‑2856580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |