Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,915,105	C→T	P17S (CCG→TCG)	barA →	hybrid sensory histidine kinase, in two‑component regulatory system with UvrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,915,105	0	C	T	100.0%	198.3 / NA	61	P17S (CCG→TCG)	barA	hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/29);  total (32/29)

GTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCACCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCA  >  NC_000913/2914969‑2915234                                                                                                                                         |                                                                                                                                  gtGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACC‑TAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcga                                                                                                                                <  1:637388/139‑1 (MQ=255)    cgtcgtTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                              <  2:1341605/139‑1 (MQ=255)     gtcgtTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                             <  2:711005/139‑1 (MQ=255)                 agTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGtt                                                                                                                 >  2:883525/1‑139 (MQ=255)                           cgcCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTa                                                                                                                       <  1:1120231/123‑1 (MQ=255)                           cgcCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTa                                                                                                                       >  2:1120231/1‑123 (MQ=255)                            gcCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGt                                                                                                      <  1:101180/139‑1 (MQ=255)                            gcCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGt                                                                                                      <  1:883525/139‑1 (MQ=255)                             ccATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                              <  2:47106/114‑1 (MQ=255)                             ccATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                              >  1:47106/1‑114 (MQ=255)                             ccATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTa                                                                                                     >  2:277106/1‑139 (MQ=255)                                ttGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGt                                                                                                                  >  2:1044968/1‑123 (MQ=255)                                ttGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGt                                                                                                                  <  1:1044968/123‑1 (MQ=255)                                                          tgtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                    >  2:813585/1‑127 (MQ=255)                                                          tgtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                    <  1:813585/127‑1 (MQ=255)                                                          tgtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGAc                                                                        >  1:913292/1‑139 (MQ=255)                                                           gtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                              <  1:433757/84‑1 (MQ=255)                                                           gtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                              >  2:433757/1‑84 (MQ=255)                                                            tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                      <  2:1327023/91‑1 (MQ=255)                                                            tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                      >  1:1327023/1‑91 (MQ=255)                                                            tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACtt                                                                      >  1:1203329/1‑139 (MQ=255)                                                            tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACtt                                                                      >  2:295926/1‑139 (MQ=255)                                                                   aaTTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGt                                                               <  2:913292/139‑1 (MQ=255)                                                                    aTTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTc                                                              >  1:1295042/1‑139 (MQ=255)                                                                      tGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCaa                                                            <  1:1208072/139‑1 (MQ=255)                                                                             tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgac                                                                                                                               >  2:85942/1‑65 (MQ=255)                                                                             tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgac                                                                                                                               <  1:85942/65‑1 (MQ=255)                                                                             tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTg                                                                                                         <  1:457342/87‑1 (MQ=255)                                                                             tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTg                                                                                                         >  2:457342/1‑87 (MQ=255)                                                                             tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAg                                                     >  2:797662/1‑139 (MQ=255)                                                                                  gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTa                                                                                                               >  1:534885/1‑76 (MQ=255)                                                                                  gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTa                                                                                                               <  2:534885/76‑1 (MQ=255)                                                                                       ccATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCTGGTGc                                           <  2:1116809/139‑1 (MQ=255)                                                                                       ccATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGc                                           <  1:295926/139‑1 (MQ=255)                                                                                         aTGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTAtt                                                                                                             >  1:611706/1‑71 (MQ=255)                                                                                         aTGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTAtt                                                                                                             <  2:611706/71‑1 (MQ=255)                                                                                             ccAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCat                                     >  1:1016713/1‑139 (MQ=255)                                                                                             ccAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCat                                     >  2:694503/1‑139 (MQ=255)                                                                                                aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                      >  2:451546/1‑55 (MQ=255)                                                                                                aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                      <  1:451546/55‑1 (MQ=255)                                                                                                aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTg                                                                                                                    >  1:1249284/1‑57 (MQ=255)                                                                                                aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTg                                                                                                                    <  2:1249284/57‑1 (MQ=255)                                                                                                aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                    <  2:531988/89‑1 (MQ=255)                                                                                                aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                    >  1:531988/1‑89 (MQ=255)                                                                                                  tACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTg                                >  1:1304802/1‑139 (MQ=255)                                                                                                         tGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                             >  2:1239996/1‑39 (MQ=255)                                                                                                         tGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                             <  1:1239996/39‑1 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGt                                                                                                                  <  1:393006/48‑1 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGt                                                                                                                  >  2:393006/1‑48 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTAtt                                                                                                             >  1:567582/1‑53 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTAtt                                                                                                             <  2:567582/53‑1 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCt                                                                                                          >  2:180014/1‑56 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCt                                                                                                          <  1:180014/56‑1 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTtcg                                                                                            >  2:273514/1‑70 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTtcg                                                                                            <  1:273514/70‑1 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCg                                                                >  2:994021/1‑98 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCg                                                                <  1:994021/98‑1 (MQ=255)                                                                                                           cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTCAGCCGCTTg                       >  1:861616/1‑139 (MQ=255)                                                                                                            gcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGc                      >  2:944080/1‑139 (MQ=255)                                                                                                               cacgcaTGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGt                   <  1:944080/139‑1 (MQ=255)                                                                                                                    atgatgATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCtg              <  1:39092/139‑2 (MQ=255)                                                                                                                    atgatgATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTa              <  2:1016713/139‑1 (MQ=255)                                                                                                                    atgatgATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTa              <  1:277106/139‑1 (MQ=255)                                                                                                                              tGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                    >  2:699253/1‑59 (MQ=255)                                                                                                                              tGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                    <  1:699253/59‑1 (MQ=255)                                                                                                                                aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATTTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCa  >  2:604942/1‑139 (MQ=255)                                                                                                                                    tGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGcc                                                >  2:527583/1‑89 (MQ=255)                                                                                                                                    tGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGcc                                                <  1:527583/89‑1 (MQ=255)                                                                                                                                     ggCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                    >  2:533161/1‑52 (MQ=255)                                                                                                                                     ggCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                    <  1:533161/52‑1 (MQ=255)                                                                                                                                     ggCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGc                                                                 <  2:393254/71‑1 (MQ=255)                                                                                                                                     ggCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGc                                                                 >  1:393254/1‑71 (MQ=255)                                                                                                                                         |                                                                                                                                  GTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCACCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCA  >  NC_000913/2914969‑2915234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑