Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,929,455	C→T	N409N (AAC→AAT)	sdaC →	putative serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,929,455	0	C	T	100.0%	182.9 / NA	57	N409N (AAC→AAT)	sdaC	putative serine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/27);  total (30/27)

ACCCTGAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAACGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGAT  >  NC_000913/2929321‑2929580                                                                                                                                       |                                                                                                                              aCCCTGAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTAt                                                                                                                           >  2:1071903/1‑139 (MQ=255)      gAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGtt                                                                                                                      >  2:572265/1‑139 (MQ=255)             aGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGATGTCGTGa                                                                                                               >  2:1325832/1‑139 (MQ=255)               cATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATg                                                                                                             <  2:205469/139‑1 (MQ=255)                  ccTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGt                                                                                                          <  1:432317/139‑1 (MQ=255)                             ttGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAAt                                                                                               >  1:938989/1‑139 (MQ=255)                                  aCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCg                                                                                          >  1:648315/1‑139 (MQ=255)                                      tGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTc                                                                                                         >  2:1224540/1‑120 (MQ=255)                                      tGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTc                                                                                                         <  1:1224540/120‑1 (MQ=255)                                      tGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                      <  2:938989/139‑1 (MQ=255)                                      tGGGCGGCCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                      >  2:1002605/1‑139 (MQ=255)                                           ggTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAtcttct                                                                                 <  2:648315/139‑1 (MQ=255)                                             tCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTAc                                                                               >  1:695065/1‑139 (MQ=255)                                              ccAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTAct                                                                              <  2:695065/139‑1 (MQ=255)                                                       gcgATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGt                                                                                                                    <  1:196062/92‑1 (MQ=255)                                                       gcgATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGt                                                                                                                    >  2:196062/1‑92 (MQ=255)                                                              tCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGt                                                                                                                    <  2:350414/85‑1 (MQ=255)                                                              tCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGt                                                                                                                    >  1:350414/1‑85 (MQ=255)                                                                       tGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTcgcg                                                     <  1:429184/139‑1 (MQ=255)                                                                        gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTActc                                                                             <  2:697623/114‑1 (MQ=255)                                                                        gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTActc                                                                             >  1:697623/1‑114 (MQ=255)                                                                        gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCt                                                                  >  1:1088970/1‑125 (MQ=255)                                                                        gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCt                                                                  <  2:1088970/125‑1 (MQ=255)                                                                         aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTg                                                                                                                >  2:123219/1‑78 (MQ=255)                                                                         aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTg                                                                                                                <  1:123219/78‑1 (MQ=255)                                                                         aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTActct                                                                            >  2:287388/1‑114 (MQ=255)                                                                         aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTActct                                                                            <  1:287388/114‑1 (MQ=255)                                                                         aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGcgcc                                                   >  1:114978/1‑139 (MQ=255)                                                                           gCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGcgccgc                                                 >  2:532348/1‑139 (MQ=255)                                                                             cGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCtt                                               >  1:760031/1‑139 (MQ=255)                                                                             cGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCtt                                               >  1:669295/1‑139 (MQ=255)                                                                             cGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTACAATCTCCGCAATCTTCTActct                                                                            >  1:550490/1‑110 (MQ=255)                                                                             cGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTACAATCTCCGCAATCTTCTActct                                                                            <  2:550490/110‑1 (MQ=255)                                                                                         ttCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                      <  1:210317/88‑1 (MQ=255)                                                                                         ttCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                      >  2:210317/1‑88 (MQ=255)                                                                                                        cAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGCGCGGCGCTTCCTCCGTTTTAGcg                    >  1:394917/1‑139 (MQ=255)                                                                                                         aaTGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAAcgc                   <  2:114978/139‑1 (MQ=255)                                                                                                            gCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCt                                                                  <  1:602914/89‑1 (MQ=255)                                                                                                            gCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCt                                                                  >  2:602914/1‑89 (MQ=255)                                                                                                            gCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGAt                >  1:486646/1‑139 (MQ=255)                                                                                                                aaGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTAt            <  1:532348/139‑1 (MQ=255)                                                                                                                    aCAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAGCGCGATGTATTTcc        <  2:394917/139‑1 (MQ=255)                                                                                                                     cAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGCCTGATTGCAATCTCCGCAATCTTCTAGTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAATGCGATGTATTTCCt       <  1:817423/139‑1 (MQ=255)                                                                                                                     cAGCGGTCACATCAGCAATGTATTCGTTGCCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCt       <  1:572265/139‑1 (MQ=255)                                                                                                                      aGCGGTCACATCAGCAATGTATTCGTTGTCGTGAt                                                                                                              <  1:1338177/35‑1 (MQ=255)                                                                                                                      aGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCg                                                                                          >  2:1297241/1‑55 (MQ=255)                                                                                                                      aGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCg                                                                                          <  1:1297241/55‑1 (MQ=255)                                                                                                                      aGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTcgc                                                      >  1:1081830/1‑91 (MQ=255)                                                                                                                      aGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTcgc                                                      <  2:1081830/91‑1 (MQ=255)                                                                                                                      aGCGGTCACATCAGCAATGCATTCGTTGTCGTGAt                                                                                                              >  2:1338177/1‑35 (MQ=25)                                                                                                                        cGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATg    >  1:850032/1‑139 (MQ=255)                                                                                                                          gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                        <  1:1147753/53‑1 (MQ=255)                                                                                                                          gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                        >  2:1147753/1‑53 (MQ=255)                                                                                                                          gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGAt  <  2:669295/139‑1 (MQ=255)                                                                                                                          gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGAt  >  2:860469/1‑139 (MQ=255)                                                                                                                           tCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGgcggcg                                     >  1:1222286/1‑103 (MQ=255)                                                                                                                           tCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGgcggcg                                     <  2:1222286/103‑1 (MQ=255)                                                                                                                                       |                                                                                                                              ACCCTGAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAACGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGAT  >  NC_000913/2929321‑2929580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑