Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,962,441	C→T	*108* (TAG→TAA)	ppdC ←	putative prepilin peptidase‑dependent protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,962,441	0	C	T	100.0%	157.4 / NA	49	*108* (TAG→TAA)	ppdC	putative prepilin peptidase‑dependent protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/23);  total (26/23)

ACCGGTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCG  >  NC_000913/2962306‑2962574                                                                                                                                        |                                                                                                                                      aCCGGTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGattac                                                                                                                                    >  1:337543/1‑139 (MQ=255)  ccGGTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGattact                                                                                                                                   <  2:687855/139‑1 (MQ=255)           tGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGAt                                                                                                                             <  2:402299/136‑1 (MQ=255)           tGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGAt                                                                                                                             >  1:402299/1‑136 (MQ=255)             cACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTATCATAGCGGCTCCTGATTACTGCCGATTCggg                                                                                                                        <  2:231609/139‑1 (MQ=255)                              gTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCAt                                                                                                       >  1:212090/1‑139 (MQ=255)                              gTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCAt                                                                                                       >  1:515174/1‑139 (MQ=255)                               ttCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCg                                                                                                                          <  1:618360/119‑1 (MQ=255)                               ttCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCg                                                                                                                          >  2:618360/1‑119 (MQ=255)                                         tCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCgg                                                                                                            <  2:530369/123‑1 (MQ=255)                                         tCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCgg                                                                                                            >  1:530369/1‑123 (MQ=255)                                            tCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCg                                                                                                                          <  1:872085/106‑1 (MQ=255)                                            tCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCg                                                                                                                          >  2:872085/1‑106 (MQ=255)                                               aGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      <  2:19599/139‑1 (MQ=255)                                                    ttcgcgttcgACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGt                                                                                 >  2:465264/1‑139 (MQ=255)                                                              aCAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAg                                                                                                                <  2:884384/98‑1 (MQ=255)                                                              aCAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAg                                                                                                                >  1:884384/1‑98 (MQ=255)                                                                       tCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGa                                                              >  2:372850/1‑139 (MQ=255)                                                                         cATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTTGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATg                                                            <  1:487999/139‑1 (MQ=255)                                                                         cATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCggg                                                                                  >  2:569555/1‑117 (MQ=255)                                                                         cATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCggg                                                                                  <  1:569555/117‑1 (MQ=255)                                                                          aTCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      >  1:790517/1‑112 (MQ=255)                                                                          aTCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      <  2:790517/112‑1 (MQ=255)                                                                                 cTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATg                                                            >  1:328263/1‑131 (MQ=255)                                                                                 cTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATg                                                            <  2:328263/131‑1 (MQ=255)                                                                                  ttCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACAt                                                   <  2:212090/139‑1 (MQ=255)                                                                                            tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAAc                                                                            >  1:327357/1‑104 (MQ=255)                                                                                            tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAAc                                                                            <  2:327357/104‑1 (MQ=255)                                                                                            tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         >  1:893396/1‑139 (MQ=255)                                                                                                gACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGt                                     <  2:893396/139‑1 (MQ=255)                                                                                                           tGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      <  2:1028441/79‑1 (MQ=255)                                                                                                           tGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      >  1:1028441/1‑79 (MQ=255)                                                                                                             gTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAGCTAGCGTAACGCTGATg                                                            <  2:771076/103‑1 (MQ=255)                                                                                                             gTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATg                                                            >  1:771076/1‑103 (MQ=255)                                                                                                                 aCCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAAt                                                                                                                    <  2:578361/43‑1 (MQ=255)                                                                                                                 aCCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAAt                                                                                                                    >  1:578361/1‑43 (MQ=255)                                                                                                                 aCCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAAt                                                                                                                    <  2:986015/43‑1 (MQ=255)                                                                                                                 aCCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAAt                                                                                                                    >  1:986015/1‑43 (MQ=255)                                                                                                                  cccTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTg                   <  1:465264/139‑1 (MQ=255)                                                                                                                            aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCg                                                                                                   <  2:456684/49‑1 (MQ=255)                                                                                                                            aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCg                                                                                                   >  1:456684/1‑49 (MQ=255)                                                                                                                             gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATg                                                            <  2:253755/87‑1 (MQ=255)                                                                                                                             gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATg                                                            >  1:253755/1‑87 (MQ=255)                                                                                                                             gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAg        >  2:612618/1‑139 (MQ=255)                                                                                                                             gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAg        >  2:728449/1‑139 (MQ=255)                                                                                                                              cGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCAt                                                                                                       <  1:1292043/43‑1 (MQ=255)                                                                                                                              cGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCAt                                                                                                       >  2:1292043/1‑43 (MQ=255)                                                                                                                               ggCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGt      >  1:1199874/1‑139 (MQ=255)                                                                                                                                   ccTGATTACTGACGATTCGGGCAATGCAGGCGGGTCAtc                                                                                                      >  2:639952/1‑39 (MQ=255)                                                                                                                                   ccTGATTACTGACGATTCGGGCAATGCAGGCGGGTCAtc                                                                                                      <  1:639952/39‑1 (MQ=255)                                                                                                                                   ccTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCg  >  2:1288296/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                      ACCGGTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCG  >  NC_000913/2962306‑2962574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑