Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,999,821 G→A A24V (GCG→GTG)  ygeR ← LysM domain‑containing M23 family putative peptidase, septation lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,999,8210GA93.9% 184.7 / 3.3 66A24V (GCG→GTG) ygeRLysM domain‑containing M23 family putative peptidase, septation lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (28/34);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

TCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTG  >  NC_000913/2999690‑2999944
                                                                                                                                   |                                                                                                                           
tCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAg                                                                                                                      <  2:906037/139‑1 (MQ=255)
     cgcgCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTc                                                                                                                 <  2:253439/139‑1 (MQ=255)
               cTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaa                                                                                                       <  1:1257794/139‑1 (MQ=255)
                  ttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaacaa                                                                                                    <  2:444589/139‑1 (MQ=255)
                          gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAg                                                                                                                      >  1:817067/1‑113 (MQ=255)
                          gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAg                                                                                                                      <  2:817067/113‑1 (MQ=255)
                          gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaac                                                                                                      >  1:917912/1‑129 (MQ=255)
                          gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGCAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaac                                                                                                      <  2:917912/129‑1 (MQ=255)
                                  ggtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCaga                                                                                    <  1:1080255/139‑1 (MQ=255)
                                   gtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag                                                                                   <  2:561164/139‑1 (MQ=255)
                                   gtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag                                                                                   >  2:202743/1‑139 (MQ=255)
                                    tgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAgaga                                                                                  >  1:654698/1‑139 (MQ=255)
                                      cgcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaa                                                                                                       <  2:453710/116‑1 (MQ=255)
                                      cgcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaa                                                                                                       >  1:453710/1‑116 (MQ=255)
                                                     aGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaa                                                                                                       <  2:351482/101‑1 (MQ=255)
                                                     aGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaa                                                                                                       >  1:351482/1‑101 (MQ=255)
                                                         tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGcccccc                                                                                                                           >  1:899534/1‑77 (MQ=255)
                                                         tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCAcc                                                                                                                           <  2:899534/77‑1 (MQ=255)
                                                         tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag                                                                                   >  2:1310203/1‑117 (MQ=255)
                                                         tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag                                                                                   <  1:1310203/117‑1 (MQ=255)
                                                         tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGc                                                             >  2:848863/1‑139 (MQ=255)
                                                            ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACt                                                          >  1:527704/1‑139 (MQ=255)
                                                            ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCa                                                                                                               <  1:1010085/86‑1 (MQ=255)
                                                            ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCa                                                                                                               >  2:1010085/1‑86 (MQ=255)
                                                            ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaacaa                                                                                                    <  2:1311850/97‑1 (MQ=255)
                                                            ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaacaa                                                                                                    >  1:1311850/1‑97 (MQ=255)
                                                              ccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCa                                                        <  1:202743/139‑1 (MQ=255)
                                                                ccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTc                                                                                                                 >  1:992713/1‑80 (MQ=255)
                                                                ccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTc                                                                                                                 <  2:992713/80‑1 (MQ=255)
                                                                     tCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCtt                                                 >  2:821889/1‑139 (MQ=255)
                                                                          gtgtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTc                                            <  2:654698/139‑1 (MQ=255)
                                                                            gtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACGCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGc                                          >  2:950481/1‑139 (MQ=255)
                                                                            gtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGc                                          >  1:1112432/1‑139 (MQ=255)
                                                                                     gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaacaa                                                                                                    >  1:13778/1‑72 (MQ=255)
                                                                                     gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATaacaa                                                                                                    <  2:13778/72‑1 (MQ=255)
                                                                                     gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag                                                                                   <  1:1129630/89‑1 (MQ=255)
                                                                                     gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCagag                                                                                   >  2:1129630/1‑89 (MQ=255)
                                                                                     gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAAc                                 >  2:1104598/1‑139 (MQ=255)
                                                                                       cGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACaa                               >  2:353672/1‑139 (MQ=255)
                                                                                       cGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACaa                               >  2:348698/1‑139 (MQ=255)
                                                                                         gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGa                             <  1:950481/139‑1 (MQ=255)
                                                                                         gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGa                             <  1:1104598/139‑1 (MQ=255)
                                                                                         gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGa                             <  2:1112432/139‑1 (MQ=255)
                                                                                          aaTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCaga                                                                                    >  1:1244754/1‑83 (MQ=255)
                                                                                          aaTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCaga                                                                                    <  2:1244754/83‑1 (MQ=255)
                                                                                           aTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACg                           <  1:1164626/139‑1 (MQ=255)
                                                                                            tACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGc                          <  2:527704/139‑1 (MQ=255)
                                                                                            tACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGc                          <  1:353672/139‑1 (MQ=255)
                                                                                            tACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCATTCCTCGCTTAAAAAAGAAGACGc                          <  1:821889/139‑1 (MQ=255)
                                                                                                        tCGGATGATTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagca              >  1:236722/1‑139 (MQ=255)
                                                                                                           gatgatTTGCTACCCGAACAGCCCACCAAAAGCAGTc                                                                                                                 <  1:33191/37‑1 (MQ=255)
                                                                                                           gatgatTTGCTACCCGAACAGCCCACCAAAAGCAGTc                                                                                                                 >  2:33191/1‑37 (MQ=255)
                                                                                                           gatgatTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGc                                                                    <  2:221246/82‑1 (MQ=255)
                                                                                                           gatgatTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGc                                                                    >  1:221246/1‑82 (MQ=255)
                                                                                                           gatgatTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagcc           >  2:918978/1‑139 (MQ=255)
                                                                                                             tgatTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATATTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagccag         >  2:663956/1‑139 (MQ=255)
                                                                                                              gatTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagccagc        <  1:918978/139‑1 (MQ=255)
                                                                                                               atTTGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCagccagcc       <  2:236722/139‑1 (MQ=255)
                                                                                                                tttGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagccagc        >  2:1130747/1‑137 (MQ=255)
                                                                                                                tttGCTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagccagc        <  1:1130747/137‑1 (MQ=255)
                                                                                                                    cTACCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTg  <  1:663956/139‑1 (MQ=255)
                                                                                                                      aCCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCagccagcc       <  2:762763/132‑1 (MQ=255)
                                                                                                                      aCCCGAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCagccagcc       >  1:762763/1‑132 (MQ=255)
                                                                                                                          gAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCg                                                              >  2:526235/1‑73 (MQ=255)
                                                                                                                          gAACAGCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCg                                                              <  1:526235/73‑1 (MQ=255)
                                                                                                                               gCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCa                                                                                      <  1:191007/44‑1 (MQ=255)
                                                                                                                               gCCCACCAAAAGCAGTCCAACCGATAACAACATCACGATACCCa                                                                                      >  2:191007/1‑44 (MQ=255)
                                                                                                                                   |                                                                                                                           
TCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTG  >  NC_000913/2999690‑2999944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑