Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,035,546	T→C	T246A (ACG→GCG)	prfB ←	peptide chain release factor RF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,035,546	0	T	C	100.0%	146.5 / NA	41	T246A (ACG→GCG)	prfB	peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/20);  total (21/20)

GTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGC  >  NC_000913/3035433‑3035676                                                                                                                  |                                                                                                                                   gTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATcc                                                                                                           >  2:305805/1‑139 (MQ=255)      tgtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATgcg                                                                                                                  >  2:199913/1‑127 (MQ=255)      tgtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATgcg                                                                                                                  <  1:199913/127‑1 (MQ=255)              gAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAttt                                                                                              <  2:386256/139‑1 (MQ=255)                  ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATgcg                                                                                                                  <  2:469294/115‑1 (MQ=255)                  ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATgcg                                                                                                                  >  1:469294/1‑115 (MQ=255)                    tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCa                                                                                                                >  1:832065/1‑115 (MQ=255)                    tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCa                                                                                                                <  2:832065/115‑1 (MQ=255)                    tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                     >  2:1300079/1‑126 (MQ=255)                    tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                     <  1:1300079/126‑1 (MQ=255)                    tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatat                                                                                        <  1:305805/139‑1 (MQ=255)                         cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATccgcc                                                                                                        >  1:1261410/1‑118 (MQ=255)                         cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATccgcc                                                                                                        <  2:1261410/118‑1 (MQ=255)                         cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTc                                                                                             <  1:517813/129‑1 (MQ=255)                         cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTc                                                                                             >  2:517813/1‑129 (MQ=255)                         cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaata                                                                                   <  1:708687/139‑1 (MQ=255)                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                                                           <  2:950657/122‑1 (MQ=255)                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                                                           >  1:950657/1‑122 (MQ=255)                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATAtcatcatca                                                                          >  1:1339208/1‑139 (MQ=255)                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGCACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATAtcatcatca                                                                          >  2:962475/1‑139 (MQ=255)                                             cGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                     <  1:375256/101‑1 (MQ=255)                                             cGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                     >  2:375256/1‑101 (MQ=255)                                                          ggTAATACGCACCGCAGATTTGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAg                                                  >  2:976368/1‑139 (MQ=255)                                                          ggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                       <  1:752297/70‑1 (MQ=255)                                                          ggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                       >  2:752297/1‑70 (MQ=255)                                                          ggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatca                                                                                      >  2:508388/1‑103 (MQ=255)                                                          ggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatca                                                                                      <  1:508388/103‑1 (MQ=255)                                                                 cGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacg                                           <  2:1339208/139‑1 (MQ=255)                                                                    aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                       <  1:1306119/60‑1 (MQ=255)                                                                    aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                       >  2:1306119/1‑60 (MQ=255)                                                                                gTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCgacgac                            >  1:951782/1‑139 (MQ=255)                                                                                      ttAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                          >  1:815801/1‑71 (MQ=255)                                                                                      ttAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                          <  2:815801/71‑1 (MQ=255)                                                                                             gCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATg                                                                                                                    >  1:895272/1‑38 (MQ=255)                                                                                             gCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATg                                                                                                                    <  2:895272/38‑1 (MQ=255)                                                                                              cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGAt                                                                                                             >  1:635271/1‑44 (MQ=255)                                                                                              cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGAt                                                                                                             <  2:635271/44‑1 (MQ=255)                                                                                                    gcccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaat                                                                                    >  2:546599/1‑63 (MQ=255)                                                                                                    gcccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaat                                                                                    <  1:546599/63‑1 (MQ=255)                                                                                                      ccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCtttt      >  1:1105332/1‑139 (MQ=255)                                                                                                          gcCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGc  <  1:976368/139‑1 (MQ=255)                                                                                                                  |                                                                                                                                   GTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGC  >  NC_000913/3035433‑3035676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑