Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,823	G→A	*231* (TAG→TAA)	yghT →	putative ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,823	0	G	A	100.0%	120.4 / NA	38	*231* (TAG→TAA)	yghT	putative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/20);  total (18/20)

ACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATC  >  NC_000913/3134703‑3134950                                                                                                                         |                                                                                                                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                              <  1:1040881/139‑1 (MQ=255) acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                              <  2:1100497/139‑1 (MQ=255)     ttAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCg                                                                                                          >  2:1264494/1‑139 (MQ=255)             tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                              >  2:209870/1‑127 (MQ=255)             tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                              <  1:209870/127‑1 (MQ=255)                   tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCaaaa                                                                                                                           >  2:520906/1‑108 (MQ=255)                   tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCaaaa                                                                                                                           <  1:520906/108‑1 (MQ=255)                   tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                              >  1:1045446/1‑121 (MQ=255)                   tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                              <  2:1045446/121‑1 (MQ=255)                       caACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                              >  1:330598/1‑117 (MQ=255)                       caACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                              <  2:330598/117‑1 (MQ=255)                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                              <  1:1090094/111‑1 (MQ=255)                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                              >  2:1090094/1‑111 (MQ=255)                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                        >  2:49139/1‑139 (MQ=255)                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                     <  1:49139/139‑1 (MQ=255)                                                       ggAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCa                                                                                                       <  2:849763/92‑1 (MQ=255)                                                       ggAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCa                                                                                                       >  1:849763/1‑92 (MQ=255)                                                            aGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAg                                                   >  1:971466/1‑139 (MQ=255)                                                                  tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCaaaaa                                                                                                                          >  1:1341020/1‑62 (MQ=255)                                                                  tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCaaaaa                                                                                                                          <  2:1341020/62‑1 (MQ=255)                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                       >  2:1274850/1‑139 (MQ=255)                                                                         tGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                     <  2:159023/108‑1 (MQ=255)                                                                         tGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                     >  1:159023/1‑108 (MQ=255)                                                                              aaaaaGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                               >  2:1124670/1‑77 (MQ=255)                                                                              aaaaaGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                               <  1:1124670/77‑1 (MQ=255)                                                                                   gCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                            <  1:1264494/139‑1 (MQ=255)                                                                                                 tATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgt                       >  1:1239310/1‑130 (MQ=255)                                                                                                 tATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgt                       <  2:1239310/130‑1 (MQ=255)                                                                                                    gACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATAcc           <  2:971466/139‑1 (MQ=255)                                                                                                    gACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATAcc           <  1:1274850/139‑1 (MQ=255)                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                     <  2:646732/72‑1 (MQ=255)                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                     >  1:646732/5‑76 (MQ=255)                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                <  1:78673/77‑1 (MQ=255)                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                >  2:78673/5‑81 (MQ=255)                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                      <  1:1182082/119‑1 (MQ=255)                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                      >  2:1182082/5‑123 (MQ=255)                                                                                                          cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc      >  1:702209/5‑139 (MQ=255)                                                                                                              ggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATc  <  2:229982/139‑1 (MQ=255)                                                                                                                         |                                                                                                                                ACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATC  >  NC_000913/3134703‑3134950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑