Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,199,429	T→C	A71A (GCA→GCG)	glnE ←	fused deadenylyltransferase/adenylyltransferase for glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,199,429	0	T	C	100.0%	124.5 / NA	35	A71A (GCA→GCG)	glnE	fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/15);  total (20/15)

AATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCT  >  NC_000913/3199295‑3199557                                                                                                                                       |                                                                                                                                 aaTATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCcg                                                                                                                              <  1:690067/139‑1 (MQ=255)        tctctTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAAtg                                                                                                                       <  1:380300/139‑1 (MQ=255)            ttCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAAtg                                                                                                                       <  1:852766/135‑1 (MQ=255)            ttCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAAtg                                                                                                                       >  2:852766/1‑135 (MQ=255)                  aaCCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATtcg                                                                                                             >  2:367995/1‑139 (MQ=255)                      aGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCgg                                                                                                         >  2:1092049/1‑139 (MQ=255)                        tGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATtcgt                                                                                                            <  1:1110653/134‑1 (MQ=255)                        tGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATtcgt                                                                                                            >  2:1110653/1‑134 (MQ=255)                         gCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTaa                                                                                                                         <  1:1225842/120‑1 (MQ=255)                         gCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTaa                                                                                                                         >  2:1225842/1‑120 (MQ=255)                           cAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTgc                                                                                                    >  1:919556/1‑139 (MQ=255)                                         aGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATtcgtc                                                                                                           <  1:332450/118‑1 (MQ=255)                                         aGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATtcgtc                                                                                                           >  2:332450/1‑118 (MQ=255)                                            cGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACACAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAg                                                                                   <  2:919556/139‑1 (MQ=255)                                                          aTGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCAct                                                                     <  1:367995/139‑1 (MQ=255)                                                                           ccGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGTGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg                                                    <  1:1092049/139‑1 (MQ=255)                                                                           ccGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCACAATCACg                                                    <  1:413729/139‑1 (MQ=255)                                                                               aGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGt                                                >  2:1195728/1‑139 (MQ=255)                                                                                                 gCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTaa                              >  1:48349/1‑139 (MQ=255)                                                                                                      gTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAaagta                         >  2:921343/1‑139 (MQ=255)                                                                                                             acaTTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTg                  >  1:189854/1‑139 (MQ=255)                                                                                                                     gagCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATg                                                              >  1:766468/1‑87 (MQ=255)                                                                                                                     gagCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATg                                                              <  2:766468/87‑1 (MQ=255)                                                                                                                     gagCGCCGCCTGCAACCACGGCGCGTAATGCTGCCATTCGTTGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCGCGGTGTCCTGCACAAAATCACTAAGAGTAAGTACTGACTTCgtc          <  2:485482/139‑3 (MQ=255)                                                                                                                       gcgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCtg        >  1:952739/1‑139 (MQ=255)                                                                                                                        cgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCttt                                                                                       <  1:762402/59‑1 (MQ=255)                                                                                                                        cgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCttt                                                                                       >  2:762402/1‑59 (MQ=255)                                                                                                                        cgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCtgt       >  2:239552/1‑139 (MQ=255)                                                                                                                        cgcATCCTGCAACCACGCCGCGTAATGCTGCAATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCtgt       >  1:485482/1‑139 (MQ=255)                                                                                                                         gcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCtgtg      >  1:882725/1‑139 (MQ=255)                                                                                                                          cctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGt                                                                                              <  2:528408/50‑1 (MQ=255)                                                                                                                          cctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGt                                                                                              >  1:528408/1‑50 (MQ=255)                                                                                                                           ctcctGCAACCACTCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCTGTACGCTTTACACTTCCGTCAACCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTgcg    >  2:1158878/1‑139 (MQ=255)                                                                                                                           ctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTgcg    <  2:189854/139‑1 (MQ=255)                                                                                                                            tcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTgcgc   <  2:612993/139‑1 (MQ=255)                                                                                                                             cctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCt  >  2:1088785/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                 AATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCT  >  NC_000913/3199295‑3199557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑