Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,255,020	G→A	*234* (TAG→TAA)	yhaK →	redox‑sensitive bicupin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,255,020	0	G	A	100.0%	142.1 / NA	45	*234* (TAG→TAA)	yhaK	redox‑sensitive bicupin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/21);  total (24/21)

ATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGC  >  NC_000913/3254885‑3255155                                                                                                                                        |                                                                                                                                        aTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAattg                                                                                                                                      >  2:377680/1‑139 (MQ=255) aTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAattg                                                                                                                                      >  1:989927/1‑139 (MQ=255) aTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAattg                                                                                                                                      >  2:836461/1‑139 (MQ=255)         cgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACa                                                                                                                              <  1:605687/139‑1 (MQ=255)          gcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                                             <  2:392559/139‑1 (MQ=255)                      tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                                                 <  1:38068/139‑1 (MQ=255)                               aGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACa                                                                                                                              <  2:722766/117‑1 (MQ=255)                               aGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACa                                                                                                                              >  1:722766/1‑117 (MQ=255)                                                 tGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                                             <  2:486315/100‑1 (MQ=255)                                                 tGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                                             >  1:486315/1‑100 (MQ=255)                                                   aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAggg                                                                                                                           <  2:754029/100‑1 (MQ=255)                                                   aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAggg                                                                                                                           >  1:754029/1‑100 (MQ=255)                                                        gCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTg                                                                               >  2:912024/1‑139 (MQ=255)                                                             tATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                                                 >  1:374838/1‑100 (MQ=255)                                                             tATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                                                 <  2:374838/100‑1 (MQ=255)                                                                             aaCATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGaa                                                          <  1:377680/139‑1 (MQ=255)                                                                                     gCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                                             <  2:1234904/64‑1 (MQ=255)                                                                                     gCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAg                                                                                                                             >  1:1234904/1‑64 (MQ=255)                                                                                       tGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCa                                                <  1:836461/139‑1 (MQ=255)                                                                                        ggTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCAc                                               >  2:400552/1‑139 (MQ=255)                                                                                                         tGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCt                              >  1:1021437/1‑139 (MQ=255)                                                                                                          gcgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtgg                                                                     <  1:567873/99‑1 (MQ=255)                                                                                                          gcgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtgg                                                                     >  2:567873/1‑99 (MQ=255)                                                                                                           cgcgcTTTGCTGCTAGATTTGTCTGTCTAATTGTTTTTACAGGGAgatgatg                                                                                                                   <  1:282948/52‑1 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTa                                                                                                                                >  1:785181/1‑39 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTa                                                                                                                                <  2:785181/39‑1 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAgg                                                                                                                            >  2:53330/1‑43 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAgg                                                                                                                            <  1:53330/43‑1 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                                                 <  2:414651/54‑1 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                                                 >  1:414651/1‑54 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatg                                                                                                                   >  2:282948/1‑52 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcg                                                                                         >  2:695457/1‑78 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcg                                                                                         <  1:695457/78‑1 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTAt                            >  1:557736/1‑139 (MQ=255)                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTAt                            >  1:231174/1‑139 (MQ=255)                                                                                                            gcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                                                 >  1:1027548/1‑53 (MQ=255)                                                                                                            gcgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAgatgatgat                                                                                                                 <  2:1027548/53‑1 (MQ=255)                                                                                                             cgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGGCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGa                          >  2:964102/1‑139 (MQ=255)                                                                                                             cgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggtg                                                                   <  2:336901/98‑1 (MQ=255)                                                                                                             cgcTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggtg                                                                   >  1:336901/1‑98 (MQ=255)                                                                                                                tttGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAaga                       >  2:832359/1‑139 (MQ=255)                                                                                                                   gCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggt                                                                    >  2:1310703/1‑91 (MQ=255)                                                                                                                   gCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggt                                                                    <  1:1310703/91‑1 (MQ=255)                                                                                                                          gATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGAAGAGTAAAAAATAGGCCAAAAAGCGCCAGCTGGTGAAGCCCGTGGTGGCGCAAGAACCTGCTCGCACCGCCAAAAATTCTGGCGATGAAGAGATGTTgagc             >  2:185797/1‑139 (MQ=255)                                                                                                                           aTTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcg            <  1:198737/139‑1 (MQ=255)                                                                                                                                 cTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTgg      >  2:943177/1‑139 (MQ=255)                                                                                                                                   gTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGaa    >  1:1173581/1‑139 (MQ=255)                                                                                                                                     cTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGc  <  1:912024/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGC  >  NC_000913/3254885‑3255155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑