Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,296,984 G→A *192* (TAG→TAA yraP → outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,296,9840GA100.0% 80.3 / NA 27*192* (TAG→TAAyraPouter membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

TGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAAC  >  NC_000913/3296848‑3297107
                                                                                                                                        |                                                                                                                           
tGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGTAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                           <  2:960551/139‑1 (MQ=255)
tGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                           <  1:859473/139‑1 (MQ=255)
tGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGACTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                           <  1:767078/139‑1 (MQ=255)
   aaGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGcc                                                                                                                        <  1:1223176/139‑1 (MQ=255)
                              tCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGc                                                                                             <  2:21182/139‑1 (MQ=255)
                               ccTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaaca                                                                                                                           <  1:1288351/108‑1 (MQ=255)
                                       ggCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                    >  1:994693/1‑139 (MQ=255)
                                             tGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                              >  2:506835/1‑139 (MQ=255)
                                                 tGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                          >  1:848859/1‑139 (MQ=255)
                                                   aaCGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaa                                                                        >  1:1222294/1‑139 (MQ=255)
                                                    aCGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAc                                                                       <  2:848859/139‑1 (MQ=255)
                                                                     cggcAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTGCGGCGTTTACGTTTATTAAATaacagc                                                                                                                         >  2:953711/1‑72 (MQ=255)
                                                                     cggcAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATaacagc                                                                                                                         <  1:953711/72‑1 (MQ=255)
                                                                           atatTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATaa                                                <  2:994693/139‑1 (MQ=255)
                                                                           atatTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATaa                                                <  1:506835/139‑1 (MQ=255)
                                                                             atTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGt                                                                                                                  <  2:1188244/71‑1 (MQ=255)
                                                                             atTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGt                                                                                                                  >  1:1188244/1‑71 (MQ=255)
                                                                                    gCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAAcc                                                             <  1:646565/117‑1 (MQ=255)
                                                                                    gCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAAcc                                                             >  2:646565/1‑117 (MQ=255)
                                                                                        ggTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTAcgccg                                   <  1:1083442/139‑1 (MQ=255)
                                                                                         gTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGcc                                                                                                                        >  1:268111/1‑53 (MQ=255)
                                                                                         gTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGcc                                                                                                                        <  2:268111/53‑1 (MQ=255)
                                                                                          tGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                  <  2:1283432/90‑1 (MQ=255)
                                                                                          tGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                  >  1:1283432/1‑90 (MQ=255)
                                                                                           gAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACa                                                                         >  2:204718/1‑98 (MQ=255)
                                                                                           gAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACa                                                                         <  1:204718/98‑1 (MQ=255)
                                                                                                gcgTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGAc                           <  2:1222294/139‑1 (MQ=255)
                                                                                                           gTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGt                                                                                        >  2:977291/1‑67 (MQ=255)
                                                                                                           gTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGt                                                                                        <  1:977291/67‑1 (MQ=255)
                                                                                                           gTAACTACGGCGTTTACGTTTATTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCt                >  2:603305/1‑139 (MQ=255)
                                                                                                                         tacgtttaTTAAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGaacaac  >  2:86693/1‑139 (MQ=255)
                                                                                                                                attaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACa                                                                         <  1:922231/61‑1 (MQ=255)
                                                                                                                                attaAATAACAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACa                                                                         >  2:922231/1‑61 (MQ=255)
                                                                                                                                        |                                                                                                                           
TGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAAC  >  NC_000913/3296848‑3297107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: