| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,301,599 | G→A | G39S (GGC→AGC) | yhbU → | U32 peptidase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,301,599 | 0 | G | A | 91.9% | 94.6 / ‑0.1 | 37 | G39S (GGC→AGC) | yhbU | U32 peptidase family protein |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (17/17); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCC‑ATCGAAAACGGCGCAGATGC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGT > NC_000913/3301487‑3301726 | ggAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCC‑ATCGAAAACGGCGCAGATGC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTg > 2:1066137/1‑139 (MQ=255) tgctCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCC‑ATCGAAAACGGCGCAGATGC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGa < 1:23105/139‑1 (MQ=255) ctctGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCC‑ATCGAAAACGGCGCAGATGC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGc > 1:98051/1‑108 (MQ=255) gccGGAAATCTCCCGGCGCTTAAGGCGGCC‑ATCGAAAACGGCGCAGATGC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtt < 1:1066137/139‑1 (MQ=255) ccGGAAATCTCCCGGCGCTTAAGGCGGCC‑ATCGAAAACGGCGCAGATGC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTACCGGAAAAAAATTTCCAGAAAGCGTTAAGttt > 1:581682/1‑139 (MQ=255) ggAAATTTCCCGGCGGTTTAGGCCGCCAATCGAAAACGG‑GGAAATGCTTGTTATTATCGGGTTAAAGGATGTTACCATTCCCCTTCACTTGCCAGGCCTT‑ACCTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTg < 2:581682/139‑1 (MQ=255) aaTCTCCCGGCGCTTAAGGCGGCC‑ATCGAAAACGGCGCAGATGC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTcc > 1:1331292/1‑139 (MQ=255) gcC‑ATCGAAAACGGCGCAGATGC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTc < 1:1050199/139‑1 (MQ=255) aaaCGGCGCAGATGC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcgcg < 2:1331292/139‑1 (MQ=255) gC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTg < 1:628785/139‑1 (MQ=255) gTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGc < 2:282981/73‑1 (MQ=255) gTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGc > 1:282981/1‑73 (MQ=255) tatCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCg > 1:13095/1‑139 (MQ=255) tatCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGACCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCg > 1:890770/1‑139 (MQ=255) gggCTAAAAGATGGTACCAATGCCTGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa < 2:951170/69‑1 (MQ=255) gggCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa > 1:951170/1‑69 (MQ=255) gggCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcg < 1:1216994/112‑1 (MQ=255) gggCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcg > 2:1216994/1‑112 (MQ=255) gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCa > 1:26290/1‑63 (MQ=255) gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCa < 2:26290/63‑1 (MQ=255) gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGt > 1:584162/1‑139 (MQ=255) gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGt > 1:310527/1‑139 (MQ=255) tAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTa < 2:584162/139‑1 (MQ=255) tgatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCAt > 1:190730/1‑121 (MQ=255) tgatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCAt < 2:190730/121‑1 (MQ=255) aaTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgc < 2:13095/139‑1 (MQ=255) tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcg > 1:85643/1‑92 (MQ=255) tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcg < 2:85643/92‑1 (MQ=255) ccGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGcc < 2:1315204/139‑1 (MQ=255) ccGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGcc < 2:310527/139‑1 (MQ=255) cGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTa < 2:415236/119‑1 (MQ=255) cGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTa > 1:415236/1‑119 (MQ=255) gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa < 2:849730/44‑1 (MQ=255) gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa > 1:849730/1‑44 (MQ=255) gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCaa > 2:1201440/1‑76 (MQ=255) gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCaa < 1:1201440/76‑1 (MQ=255) gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGt > 2:71156/1‑139 (MQ=255) | GGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCC‑ATCGAAAACGGCGCAGATGC‑TGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGT > NC_000913/3301487‑3301726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |