Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,308,040	C→T	*295* (TAG→TAA)	nlpI ←	lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,308,040	0	C	T	100.0%	182.7 / NA	58	*295* (TAG→TAA)	nlpI	lipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/28);  total (30/28)

TCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCT  >  NC_000913/3307905‑3308171                                                                                                                                        |                                                                                                                                    tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTAt                                                                                                                                  >  2:708753/1‑139 (MQ=255) tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTAt                                                                                                                                  >  2:863669/1‑139 (MQ=255)   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTg                                                                                                                                >  1:756639/1‑139 (MQ=255)    tcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGc                                                                                                                               <  2:203104/139‑1 (MQ=255)                   gCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGcc                                                                                                                <  2:162410/139‑1 (MQ=255)                      aTTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAgg                                                                                                             >  1:931125/1‑139 (MQ=255)                       ttttCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCACAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGt                                                                                                            <  1:291006/139‑1 (MQ=255)                       ttttCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGt                                                                                                            <  2:931125/139‑1 (MQ=255)                              tGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTg                                                                                                     >  1:1223447/1‑139 (MQ=255)                                  aaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                 <  2:793930/139‑1 (MQ=255)                                               cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                    >  2:133658/1‑139 (MQ=255)                                               cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                    <  2:923415/139‑1 (MQ=255)                                                         gtgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaa                                                                          >  1:523064/1‑139 (MQ=255)                                                           gtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaaca                                                                        <  1:863669/139‑1 (MQ=255)                                                                aaCGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCg                                                                   >  2:383733/1‑139 (MQ=255)                                                                   gAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTAt                                                                <  2:1223447/139‑1 (MQ=255)                                                                      caacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATaa                                                                                <  1:501998/120‑1 (MQ=255)                                                                      caacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATaa                                                                                >  2:501998/1‑120 (MQ=255)                                                                      caacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCgg                                                             >  2:294628/1‑139 (MQ=255)                                                                      caacaacGCCCTCACACGATAAGGAGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGCCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCgg                                                             >  2:883068/1‑139 (MQ=255)                                                                        acaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTg                                                           <  1:278473/139‑1 (MQ=255)                                                                             gCCCTCACCCGTTAAGGTGATTGCAATCAAAAAAGAATACGCGCTGATGTGTGCGACAGTTATTGCTGGTCCGATTCTGCCATGTCATTTTGGATCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGATGCTcaa                                                      >  1:173235/1‑139 (MQ=255)                                                                             gCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaa                                                      <  1:133658/139‑1 (MQ=255)                                                                              cccTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaac                                                     <  2:523064/139‑1 (MQ=255)                                                                                 tCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTAt                                                                >  2:948428/1‑125 (MQ=255)                                                                                 tCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTAt                                                                <  1:948428/125‑1 (MQ=255)                                                                                        ttAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGa                                           >  2:38101/1‑139 (MQ=255)                                                                                        ttAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGa                                           >  2:908861/1‑139 (MQ=255)                                                                                         tAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGt                                                                                                                           <  2:700667/58‑1 (MQ=255)                                                                                         tAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGt                                                                                                                           >  1:700667/1‑58 (MQ=255)                                                                                         tAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                          <  1:294628/139‑1 (MQ=255)                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTACAACAATGCGTATCGGTGCTCAACAAAGTTATGAACgttgt                                    >  2:806015/1‑139 (MQ=255)                                                                                                 tGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAg                                                                                                              <  2:950757/63‑1 (MQ=255)                                                                                                 tGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAg                                                                                                              >  1:950757/1‑63 (MQ=255)                                                                                                 tGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGttgttg                                  >  1:915733/1‑139 (MQ=255)                                                                                                  ggCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                       <  2:1319024/69‑1 (MQ=255)                                                                                                  ggCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                       >  1:1319024/1‑69 (MQ=255)                                                                                                        cAAAAAAGATTACGGGCTGAGGTGTACGTCAGTTATTGCTGTGCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAAAGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAAcgg                           <  2:808238/139‑3 (MQ=255)                                                                                                          aaaaaGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGcc                         >  1:1016946/1‑139 (MQ=255)                                                                                                                 ttACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGa                  >  1:180928/1‑139 (MQ=255)                                                                                                                  tACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa                 >  1:84009/1‑139 (MQ=255)                                                                                                                    cGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaaaa               <  2:173235/139‑3 (MQ=255)                                                                                                                          gATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCg         <  1:708753/139‑1 (MQ=255)                                                                                                                            tgtgTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                 >  2:104254/1‑49 (MQ=255)                                                                                                                            tgtgTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                 <  1:104254/49‑1 (MQ=255)                                                                                                                               gtACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                    <  1:258029/59‑1 (MQ=255)                                                                                                                               gtACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                    >  2:258029/1‑59 (MQ=255)                                                                                                                               gtACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgcg    <  1:908861/139‑1 (MQ=255)                                                                                                                                tACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgcgc   <  2:756639/139‑1 (MQ=255)                                                                                                                                 aCGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                    <  2:1105964/57‑1 (MQ=255)                                                                                                                                 aCGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                    >  1:1105964/1‑57 (MQ=255)                                                                                                                                 aCGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCt  >  2:328397/1‑139 (MQ=255)                                                                                                                                  cGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                                                  >  1:945188/1‑58 (MQ=255)                                                                                                                                  cGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                                                  <  2:945188/58‑1 (MQ=255)                                                                                                                                     cAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                          <  1:879034/95‑1 (MQ=255)                                                                                                                                     cAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                          >  2:879034/1‑95 (MQ=255)                                                                                                                                     cAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACg                                        <  1:1335594/97‑1 (MQ=255)                                                                                                                                     cAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACg                                        >  2:1335594/1‑97 (MQ=255)                                                                                                                                        |                                                                                                                                    TCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCT  >  NC_000913/3307905‑3308171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑