Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,309,007 G→A intergenic (‑83/+26) nlpI ← / ← pnp lipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,309,0070GA100.0% 108.8 / NA 35intergenic (‑83/+26)nlpI/pnplipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/17);  total (18/17)

CGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCA  >  NC_000913/3308880‑3309132
                                                                                                                               |                                                                                                                             
cGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAgg                                                                                                                   >  1:1095531/1‑139 (MQ=255)
        cTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGAGGAGGGCAAATgg                                                                                                           <  2:588020/139‑1 (MQ=255)
                   aaaCACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTc                                                                                                <  1:5564/139‑1 (MQ=255)
                            cgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATgg                                                                                                           >  1:18031/1‑119 (MQ=255)
                            cgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATgg                                                                                                           <  2:18031/119‑1 (MQ=255)
                            cgcAAAAAAGGCTTCATTTCCCACCCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTc                                                                                       >  1:1002342/1‑139 (MQ=255)
                                aaaaaGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAAc                                                                                                       <  2:313267/119‑1 (MQ=255)
                                aaaaaGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAAc                                                                                                       >  1:313267/1‑119 (MQ=255)
                                             ttCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCg                                                                      <  1:423758/139‑1 (MQ=255)
                                                     cccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgca                                                              >  1:220536/1‑139 (MQ=255)
                                                      ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                             <  2:1002342/139‑1 (MQ=255)
                                                              cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCg                                                                               <  2:971555/113‑1 (MQ=255)
                                                              cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCg                                                                               >  1:971555/1‑113 (MQ=255)
                                                              cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga                                                     >  1:902545/1‑139 (MQ=255)
                                                                cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                               >  2:493335/1‑95 (MQ=255)
                                                                cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                               <  1:493335/95‑1 (MQ=255)
                                                                cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                             <  2:1217689/129‑1 (MQ=255)
                                                                cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                             >  1:1217689/1‑129 (MQ=255)
                                                                cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                   >  2:255332/1‑139 (MQ=255)
                                                                cGGTTGAATGAACGTCCTGTTCCCGGTGGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                   >  1:870237/1‑139 (MQ=255)
                                                                         gaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGTCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                          <  1:846994/139‑1 (MQ=255)
                                                                         gaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                          <  2:902545/139‑1 (MQ=255)
                                                                         gaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                          <  1:255332/139‑1 (MQ=255)
                                                                            cGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGccc                                                                                            >  1:270234/1‑86 (MQ=255)
                                                                            cGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGccc                                                                                            <  2:270234/86‑1 (MQ=255)
                                                                               ccTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                  <  1:657981/77‑1 (MQ=255)
                                                                               ccTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                  >  2:657981/1‑77 (MQ=255)
                                                                                cTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                          <  1:222191/132‑1 (MQ=255)
                                                                                cTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                          >  2:222191/1‑132 (MQ=255)
                                                                                    tCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCa                                                                                                                >  1:1093512/1‑58 (MQ=255)
                                                                                    tCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCa                                                                                                                <  2:1093512/58‑1 (MQ=255)
                                                                                             cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc                                       <  2:194907/122‑1 (MQ=255)
                                                                                             cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc                                       >  1:194907/1‑122 (MQ=255)
                                                                                                        tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGGAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGccc           >  1:899909/1‑139 (MQ=255)
                                                                                                                 ggtAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCa  >  2:65353/3‑139 (MQ=255)
                                                                                                                               |                                                                                                                             
CGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCA  >  NC_000913/3308880‑3309132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: