Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,353,230 A→G L274P (CTG→CCG)  yhcC ← putative Fe‑S oxidoreductase, Radical SAM superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,353,2300AG100.0% 289.2 / NA 79L274P (CTG→CCG) yhcCputative Fe‑S oxidoreductase, Radical SAM superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/38);  total (41/38)

AGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCT  >  NC_000913/3353095‑3353364
                                                                                                                                       |                                                                                                                                      
agaAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGTCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCg                                                                                                                                     <  1:1258291/139‑1 (MQ=255)
      gCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGagcaa                                                                                                                               >  1:782164/1‑139 (MQ=255)
       cGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGagcaag                                                                                                                              >  1:973498/1‑139 (MQ=255)
          aaTTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAagcag                                                                                                                           >  2:396596/1‑139 (MQ=255)
            ttCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCg                                                                                                                         >  2:651617/1‑139 (MQ=255)
                ccTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCgg                                                                                                                     >  1:374751/1‑139 (MQ=255)
                     aCGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCCTTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGAc                                                                                                                <  2:960835/139‑1 (MQ=255)
                      cGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACg                                                                                                               >  2:1090968/1‑139 (MQ=255)
                         cttACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGgc                                                                                                            >  1:352644/3‑139 (MQ=255)
                          ttACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGgcg                                                                                                           <  2:973498/138‑1 (MQ=255)
                                  ttGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGa                                                                                                   <  1:790830/139‑1 (MQ=255)
                                     gAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAAt                                                                                                >  1:1107258/1‑139 (MQ=255)
                                      aGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATg                                                                                               <  2:1195314/139‑1 (MQ=255)
                                       ggTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGc                                                                                              >  2:765927/1‑139 (MQ=255)
                                       ggTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGc                                                                                              <  2:782164/139‑1 (MQ=255)
                                         tAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                            <  2:649694/139‑1 (MQ=255)
                                               cGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAg                                                                                      >  2:1308317/1‑139 (MQ=255)
                                                         aGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCg                                                                            <  2:1107258/139‑1 (MQ=255)
                                                         aGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCg                                                                            <  1:396596/139‑1 (MQ=255)
                                                               gATCCCTGCACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCg                                                                      <  1:539535/139‑1 (MQ=255)
                                                                aTCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGagcaag                                                                                                                              >  2:896675/1‑82 (MQ=255)
                                                                aTCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGagcaag                                                                                                                              <  1:896675/82‑1 (MQ=255)
                                                                aTCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCgg                                                                           >  2:1106229/1‑133 (MQ=255)
                                                                aTCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCgg                                                                           <  1:1106229/133‑1 (MQ=255)
                                                                aTCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCg                                                                            <  2:95657/132‑1 (MQ=255)
                                                                aTCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCg                                                                            >  1:95657/1‑132 (MQ=255)
                                                                          gCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                            <  2:1032558/106‑1 (MQ=255)
                                                                          gCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                            >  1:1032558/1‑106 (MQ=255)
                                                                            cATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                            >  1:73198/1‑104 (MQ=255)
                                                                            cATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                            <  2:73198/104‑1 (MQ=255)
                                                                            cATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCAt                                                         >  1:1164260/1‑139 (MQ=255)
                                                                            cATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCAt                                                         >  2:1321310/1‑139 (MQ=255)
                                                                            cATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCAt                                                         >  2:615516/1‑139 (MQ=255)
                                                                              tGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCAttt                                                       <  2:374751/139‑1 (MQ=255)
                                                                              tGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCAttt                                                       >  1:963294/1‑139 (MQ=255)
                                                                               gTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCggtggt                                                                                        <  1:1145908/105‑1 (MQ=255)
                                                                               gTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCggtggt                                                                                        >  2:1145908/1‑105 (MQ=255)
                                                                                  cattcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTcc                                                   <  1:1090968/139‑1 (MQ=255)
                                                                                   attcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCg                                                  >  1:1205572/1‑139 (MQ=255)
                                                                                         aTAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTg                                            >  2:1059764/1‑139 (MQ=255)
                                                                                          tAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGt                                             >  2:880214/1‑137 (MQ=255)
                                                                                          tAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGt                                             <  1:880214/137‑1 (MQ=255)
                                                                                            gCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGc                                         <  2:1205572/139‑1 (MQ=255)
                                                                                             cGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                            <  2:746042/87‑1 (MQ=255)
                                                                                             cGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                            >  1:746042/1‑87 (MQ=255)
                                                                                             cGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCg                                        >  1:249659/1‑139 (MQ=255)
                                                                                               atgcAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCTGCGTCGGGCGACGGGCGCTGGTGGAAATGc                                                                                              >  2:779882/4‑83 (MQ=255)
                                                                                               aTCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGc                                                                                              <  1:779882/83‑1 (MQ=255)
                                                                                               aTCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCgtg                                      >  1:603480/1‑139 (MQ=255)
                                                                                                  cAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGc                                                                                              >  1:1305394/1‑80 (MQ=255)
                                                                                                  cAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGc                                                                                              <  2:1305394/80‑1 (MQ=255)
                                                                                                  cAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTaa                                   <  1:1308317/139‑1 (MQ=255)
                                                                                                       cGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGgc                                                                                                            >  1:1000563/1‑61 (MQ=255)
                                                                                                       cGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGgc                                                                                                            <  2:1000563/61‑1 (MQ=255)
                                                                                                       cGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                              <  1:1321310/139‑1 (MQ=255)
                                                                                                       cGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                              <  2:1164260/139‑1 (MQ=255)
                                                                                                       cGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                              <  1:765927/139‑1 (MQ=255)
                                                                                                       cGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                              <  2:249659/139‑1 (MQ=255)
                                                                                                       cGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                              <  1:615516/139‑1 (MQ=255)
                                                                                                       cGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                              <  2:352644/139‑1 (MQ=255)
                                                                                                              tCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATg                                                                                               <  2:218003/67‑1 (MQ=255)
                                                                                                              tCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATg                                                                                               >  1:218003/1‑67 (MQ=255)
                                                                                                              tCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCa                       >  1:1314304/1‑139 (MQ=255)
                                                                                                               cccGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATg                                                                                               >  1:235844/1‑66 (MQ=255)
                                                                                                               cccGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATg                                                                                               <  2:235844/66‑1 (MQ=255)
                                                                                                               cccGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCaa                      >  1:520799/1‑139 (MQ=255)
                                                                                                                   tCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                              >  1:1282107/1‑127 (MQ=255)
                                                                                                                   tCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                              <  2:1282107/127‑1 (MQ=255)
                                                                                                                       tCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTc              >  2:260575/1‑139 (MQ=255)
                                                                                                                       tCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTc              >  1:753171/1‑139 (MQ=255)
                                                                                                                        cGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                            >  2:546952/1‑60 (MQ=255)
                                                                                                                        cGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                            <  1:546952/60‑1 (MQ=255)
                                                                                                                        cGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                              <  2:775877/122‑1 (MQ=255)
                                                                                                                        cGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                              >  1:775877/1‑122 (MQ=255)
                                                                                                                        cGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCa             >  1:1256202/1‑139 (MQ=255)
                                                                                                                                 caccacGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCg    <  2:1256202/139‑1 (MQ=255)
                                                                                                                                  accacGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGc                                                                                              >  1:397028/1‑48 (MQ=255)
                                                                                                                                  accacGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGc                                                                                              <  2:397028/48‑1 (MQ=255)
                                                                                                                                   ccacGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCt  >  1:450200/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
AGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGCT  >  NC_000913/3353095‑3353364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑