Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,366,953	G→A	G10R (GGA→AGA)	yhcF →	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,366,953	0	G	A	100.0%	317.9 / NA	97	G10R (GGA→AGA)	yhcF	putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/49);  total (48/49)

GTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTC  >  NC_000913/3366825‑3367087                                                                                                                                 |                                                                                                                                       gTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCtt                                                                                                                              >  2:567172/1‑139 (MQ=255)  ttAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                             >  1:861528/1‑139 (MQ=255)  ttAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                             <  1:287536/139‑1 (MQ=255)        tCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGcc                                                                                                                       <  2:791228/139‑1 (MQ=255)                    tGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                           <  2:1163842/139‑1 (MQ=255)                      gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:917939/139‑1 (MQ=255)                      gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:584983/139‑1 (MQ=255)                          cAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCt                                                                                                     >  2:820960/1‑139 (MQ=255)                                  aaGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGt                                                                                             >  1:436513/1‑139 (MQ=255)                                       tGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   >  1:362795/1‑128 (MQ=255)                                       tGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   <  2:362795/128‑1 (MQ=255)                                        gAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCGAGCCGCTGATAGAGTATCAAt                                                                                       <  2:691814/139‑1 (MQ=255)                                        gAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       <  1:567172/139‑1 (MQ=255)                                        gAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       <  2:683780/139‑1 (MQ=255)                                         aaGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAtt                                                                                      >  1:173580/1‑139 (MQ=255)                                             ggAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACg                                                                                  <  1:820960/139‑1 (MQ=255)                                               aaCTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgcgc                                                                                                             <  2:403443/110‑1 (MQ=255)                                               aaCTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgcgc                                                                                                             >  1:403443/1‑110 (MQ=255)                                                aCTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTa                                                                               >  1:295452/1‑139 (MQ=255)                                                   aaCCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGt                                                                                                                   >  2:951582/1‑100 (MQ=255)                                                   aaCCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGt                                                                                                                   <  1:951582/100‑1 (MQ=255)                                                                   atTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                        <  1:1209471/79‑1 (MQ=255)                                                                   atTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                        >  2:1209471/1‑79 (MQ=255)                                                                   atTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGt                                                                                                                   >  1:291643/1‑84 (MQ=255)                                                                   atTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGt                                                                                                                   <  2:291643/84‑1 (MQ=255)                                                                    ttACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCa                                                           <  2:173580/139‑1 (MQ=255)                                                                      aCAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:73170/91‑1 (MQ=255)                                                                      aCAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:73170/1‑91 (MQ=255)                                                                         ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCa                                                           >  2:505171/1‑134 (MQ=255)                                                                         ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCa                                                           <  1:505171/134‑1 (MQ=255)                                                                         ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      <  1:947783/139‑1 (MQ=255)                                                                          gCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                        <  1:1294763/72‑1 (MQ=255)                                                                          gCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                        >  2:1294763/1‑72 (MQ=255)                                                                          gCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGt                                                     >  1:464579/1‑139 (MQ=255)                                                                           cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGt                                                                           >  1:77940/1‑116 (MQ=255)                                                                           cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGt                                                                           <  2:77940/116‑1 (MQ=255)                                                                           cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    <  2:464579/139‑1 (MQ=255)                                                                            aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      >  2:1299348/1‑136 (MQ=255)                                                                            aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      <  1:1299348/136‑1 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                        >  1:116413/1‑68 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                        <  2:116413/68‑1 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                           >  2:1177932/1‑81 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                           <  1:1177932/81‑1 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                                 <  2:295452/139‑1 (MQ=255)                                                                               cTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGt                                                                           <  2:861966/112‑1 (MQ=255)                                                                               cTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTcc                                                <  2:436513/139‑1 (MQ=255)                                                                               cTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACATTAAGGt                                                                           >  1:861966/1‑112 (MQ=255)                                                                                    ggTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgcgc                                                                                                             <  1:744879/73‑1 (MQ=255)                                                                                    ggTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgcgc                                                                                                             >  2:744879/1‑73 (MQ=255)                                                                                      tCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGt                                                                                 <  1:765383/99‑1 (MQ=255)                                                                                      tCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGt                                                                                 >  2:765383/1‑99 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                           >  2:468964/1‑71 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                           <  1:468964/71‑1 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGa                                                                         <  1:597580/105‑1 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGa                                                                         >  2:597580/1‑105 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    >  2:1114183/1‑126 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    <  1:1114183/126‑1 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAg                                       >  1:548676/1‑139 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAg                                       >  2:169386/1‑139 (MQ=255)                                                                                          ggtggAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCa                                     >  2:268127/1‑139 (MQ=255)                                                                                               aGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATaga                                                                                               <  2:1061513/76‑1 (MQ=255)                                                                                               aGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATaga                                                                                               >  1:1061513/1‑76 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                        >  2:977984/1‑50 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                        >  2:291931/1‑50 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                        <  1:291931/50‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                        <  1:977984/50‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:724843/1‑65 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:724843/65‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  >  1:1113246/1‑72 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  >  2:133415/1‑72 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  <  1:133415/72‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  <  2:1113246/72‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGt                                                                                 <  2:452519/89‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGt                                                                                 >  1:452519/1‑89 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                                 >  1:524746/1‑121 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                                 <  2:524746/121‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               >  2:5967/1‑139 (MQ=255)                                                                                                 gCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       <  2:1029985/82‑1 (MQ=255)                                                                                                 gCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       >  1:1029985/1‑82 (MQ=255)                                                                                                         aaCAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGc                                      >  1:286064/1‑123 (MQ=255)                                                                                                         aaCAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGc                                      <  2:286064/123‑1 (MQ=255)                                                                                                           cAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAAc                    >  1:252919/1‑139 (MQ=255)                                                                                                                tAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:1189533/49‑1 (MQ=255)                                                                                                                tAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:1189533/1‑49 (MQ=255)                                                                                                                 aaaaTTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGcc                                                                                                        <  1:454072/49‑1 (MQ=255)                                                                                                                 aaaaTTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGcc                                                                                                        >  2:454072/1‑49 (MQ=255)                                                                                                                   aaTTACTGATTGCCAGAAGTGCCTTTTTTTCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTTACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               <  2:1289169/120‑1 (MQ=255)                                                                                                                   aaTTACTGATTGCCAGAAGTGCCTTTTTTGCCCTGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               >  1:1289169/1‑120 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  <  1:682821/48‑1 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  >  2:682821/1‑48 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTg                                                                                     <  2:1012604/61‑1 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTg                                                                                     >  1:1012604/1‑61 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    <  1:204696/94‑1 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    >  2:204696/1‑94 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATa                                            >  2:826709/1‑102 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCAGGTCAGCGCAAGCCGCTGATAGAGTATCAATTCACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATa                                            <  1:826709/102‑1 (MQ=255)                                                                                                                             tGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTc  >  2:282712/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                       GTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTC  >  NC_000913/3366825‑3367087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑