Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,414,786	C→T	P308L (CCG→CTG)	acrE →	cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,786	0	C	T	100.0%	188.0 / NA	59	P308L (CCG→CTG)	acrE	cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/31);  total (28/31)

GACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATGGTTGATTAGTGAA  >  NC_000913/3414655‑3414913                                                                                                                                    |                                                                                                                                gACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGcaacaa                                                                                                                          >  2:673167/1‑139 (MQ=255)      ttGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGt                                                                                                                     <  2:173361/139‑1 (MQ=255)      ttGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGt                                                                                                                     <  2:671663/139‑1 (MQ=255)        gatgaAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTa                                                                                                                   >  2:483592/1‑139 (MQ=255)             aaGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGc                                                                                                              <  1:673167/139‑1 (MQ=255)                cACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGcaca                                                                                                           <  1:471742/139‑1 (MQ=255)                      cTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCgt                                                                                                     <  2:794733/139‑1 (MQ=255)                           tAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGa                                                                                                <  2:94403/139‑1 (MQ=255)                             aCCCTACGTGCTGTCTTCCCTACCCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGGGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATg                                                                                              <  2:429896/139‑1 (MQ=255)                                 tACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAAc                                                                                          >  2:965681/1‑139 (MQ=255)                                   cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCgt                                                                                                     <  2:664625/126‑1 (MQ=255)                                   cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCgt                                                                                                     >  1:664625/1‑126 (MQ=255)                                   cGTGCTGTCTTCCCTAACCCGAAACATACGCTTTTGCAGGGTATGTTTTTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCg                                                                                        >  1:429896/1‑139 (MQ=255)                                               ccTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGca                                                                                                             >  2:1071300/1‑106 (MQ=255)                                               ccTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGca                                                                                                             <  1:1071300/106‑1 (MQ=255)                                                cTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTaa                                                                           <  1:998131/139‑1 (MQ=255)                                                      cGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATaa                                                                     >  1:717952/1‑139 (MQ=255)                                                        cAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGt                                                                                                                     >  2:675156/1‑89 (MQ=255)                                                        cAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGt                                                                                                                     <  1:675156/89‑1 (MQ=255)                                                        cAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCt                                                                                    >  2:190982/1‑122 (MQ=255)                                                        cAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCt                                                                                    <  1:190982/122‑1 (MQ=255)                                                             tACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAg                                                              >  2:505125/1‑139 (MQ=255)                                                                     tGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTg                                                                                      >  2:756036/1‑107 (MQ=255)                                                                     tGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTg                                                                                      <  1:756036/107‑1 (MQ=255)                                                                     tGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAgg                                                             >  2:981218/1‑132 (MQ=255)                                                                     tGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAgg                                                             <  1:981218/132‑1 (MQ=255)                                                                          ggTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGcc                                                 <  1:483592/139‑1 (MQ=255)                                                                                    tgcgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAAc                                                                                          <  1:384759/88‑1 (MQ=255)                                                                                    tgcgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAAc                                                                                          >  2:384759/1‑88 (MQ=255)                                                                                     gcgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGcc                                      >  2:248522/1‑139 (MQ=255)                                                                                      cgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGc                                                                                                                       >  2:697212/1‑57 (MQ=255)                                                                                      cgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGc                                                                                                                       <  1:697212/57‑1 (MQ=255)                                                                                      cgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATa                                                                      >  1:1321413/1‑106 (MQ=255)                                                                                      cgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATa                                                                      <  2:1321413/106‑1 (MQ=255)                                                                                        tgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCCCACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGt                                   >  2:521321/1‑139 (MQ=255)                                                                                          cACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGc                                                                                     >  2:1224703/1‑87 (MQ=255)                                                                                          cACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGc                                                                                     <  1:1224703/87‑1 (MQ=255)                                                                                          cACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATaaa                                                                    >  1:686452/1‑104 (MQ=255)                                                                                          cACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATaaa                                                                    <  2:686452/104‑1 (MQ=255)                                                                                          cACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGt                                             >  1:712925/1‑127 (MQ=255)                                                                                          cACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGt                                             <  2:712925/127‑1 (MQ=255)                                                                                          cACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGATGCCAGTCa                                 >  2:671916/1‑139 (MQ=255)                                                                                               aTTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAg                                                                                                                  <  1:705445/53‑1 (MQ=255)                                                                                               aTTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAg                                                                                                                  >  2:705445/1‑53 (MQ=255)                                                                                               aTTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGa                            <  1:505125/139‑1 (MQ=255)                                                                                                   atgaAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGt                                                                                                                     <  1:440363/46‑1 (MQ=255)                                                                                                   atgaAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGt                                                                                                                     >  2:440363/1‑46 (MQ=255)                                                                                                         gCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGcc                                                 <  2:1153647/108‑1 (MQ=255)                                                                                                         gCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGcc                                                 >  1:1153647/1‑108 (MQ=255)                                                                                                         gCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATaa                  >  1:124035/1‑139 (MQ=255)                                                                                                              cAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGCGCTGATTGTTAACGATAAATGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATGGt             >  1:1121652/1‑139 (MQ=255)                                                                                                                aCCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATGGTTg           <  2:717952/139‑1 (MQ=255)                                                                                                                      cGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTg                                              <  1:806124/98‑1 (MQ=255)                                                                                                                      cGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTg                                              >  2:806124/1‑98 (MQ=255)                                                                                                                       gCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATGGTTGATTAGTg    <  1:965681/139‑1 (MQ=255)                                                                                                                       gCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGATGCCAGTCAGGCGATTGGCGATAAATGGTTGATTAGTg    <  1:671916/139‑1 (MQ=255)                                                                                                                         cATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATGGTTGATTAGTGaa  <  2:664150/139‑1 (MQ=255)                                                                                                                               tATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAg                                                                  >  2:375236/1‑69 (MQ=255)                                                                                                                               tATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAg                                                                  <  1:375236/69‑1 (MQ=255)                                                                                                                                    |                                                                                                                                GACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATGGTTGATTAGTGAA  >  NC_000913/3414655‑3414913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑