Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,421,860 T→C L179S (TTA→TCA)  yhdY → putative amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,421,8600TC100.0% 141.6 / NA 40L179S (TTA→TCA) yhdYputative amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/19);  total (21/19)

CCACTGATTGTCTGGTGGCTGATGTATGGCGGTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTTACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTACCGTTCTGTTTATGTCTTCGGTCATGCTGCCGTTGTTTATG  >  NC_000913/3421724‑3421990
                                                                                                                                        |                                                                                                                                  
ccACTGATTGTCTGGTGGCTGATGTATGGCGGTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCAc                                                                                                                                  <  1:545610/139‑1 (MQ=255)
                ggCTGATGTATGGCGGTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTcgccg                                                                                                                  <  1:993311/139‑1 (MQ=255)
                ggCTGATGTATGGCGGTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTcgccg                                                                                                                  <  1:462003/139‑1 (MQ=255)
                    gATGTATGGCGGTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTcc                                                                                                              >  2:329482/1‑139 (MQ=255)
                        tATGGCGGTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGt                                                                                                                       >  2:555741/1‑126 (MQ=255)
                        tATGGCGGTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGt                                                                                                                       <  1:555741/126‑1 (MQ=255)
                          tGGCGGTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTcg                                                                                                                     >  1:718033/1‑126 (MQ=255)
                          tGGCGGTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTcg                                                                                                                     <  2:718033/126‑1 (MQ=255)
                              ggTTTTTTTGCTCTTGAGCGGGTTGAAGCCCGGCAATGGGGCGGGCTGACGCGAACTTTTATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGa                                                                                                    <  2:359274/139‑1 (MQ=255)
                               gTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGAt                                                                                                   >  1:1108707/1‑139 (MQ=255)
                                cttttttGCTCTTGAGCGGGTTGAAACCCGGCAATGGGTCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGACGTTAGGTGGCCGCTCCCATATGCCGAtt                                                                                                  >  2:867796/2‑139 (MQ=255)
                                                             ggCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGa                                                                     >  1:1147476/1‑139 (MQ=255)
                                                                     ggcgggcTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGgc                                                             >  2:118489/1‑139 (MQ=255)
                                                                      gcgggcTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGgcg                                                            <  1:118489/139‑1 (MQ=255)
                                                                              aCGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTa                                                                         <  1:252106/118‑1 (MQ=255)
                                                                              aCGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTa                                                                         >  2:252106/1‑118 (MQ=255)
                                                                              aCGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGc                                                    >  2:305560/1‑139 (MQ=255)
                                                                                    aCTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGTGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCt                                                >  2:1007978/1‑137 (MQ=255)
                                                                                    aCTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGTGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCt                                                <  1:1007978/137‑1 (MQ=255)
                                                                                    aCTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGa                                              >  1:275848/1‑139 (MQ=255)
                                                                                       ttAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTa                                           <  1:305560/139‑1 (MQ=255)
                                                                                            tattGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTACCGtt                                      <  2:1108707/139‑1 (MQ=255)
                                                                                            tatcGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTACCGtt                                      <  1:867796/139‑1 (MQ=255)
                                                                                               tGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTcg                                                                                                                     >  1:436026/1‑57 (MQ=255)
                                                                                               tGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTcg                                                                                                                     <  2:436026/57‑1 (MQ=255)
                                                                                                   tCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTACCGTTCTGTTTa                               >  2:267408/1‑139 (MQ=255)
                                                                                                      gTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATtgt                                                                                                >  1:608616/1‑71 (MQ=255)
                                                                                                      gTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATtgt                                                                                                <  2:608616/71‑1 (MQ=255)
                                                                                                         gggATTGCGTGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATtgtg                                                                                               >  1:903214/1‑69 (MQ=255)
                                                                                                         gggATTGCGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATtgtg                                                                                               <  2:903214/69‑1 (MQ=255)
                                                                                                                cGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTACCGTTCTGttt                                >  2:1056549/1‑125 (MQ=255)
                                                                                                                cGGGGGCGCTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTACCGTTCTGttt                                <  1:1056549/125‑1 (MQ=255)
                                                                                                                     gcgcTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCg                                                  >  2:972253/1‑102 (MQ=255)
                                                                                                                     gcgcTACCGTGGGGGATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCg                                                  <  1:972253/102‑1 (MQ=255)
                                                                                                                            tgTGGGGGATCTTGCTGGCGGTATGTATCCCATCGCATATGCCGTTTGTGCTTTTCTTATCGGTCCTTTTTGGCGTGTTCTTGCGCGGCGTGCCGCTGATTACCGTTCTGAATATGTCGTCGGTTATGCTGCCgttggt      <  2:30127/138‑3 (MQ=255)
                                                                                                                               gggggATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCg                                                  >  2:1037958/1‑92 (MQ=255)
                                                                                                                               gggggATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCg                                                  <  1:1037958/92‑1 (MQ=255)
                                                                                                                               gggggATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTACCGTTCTGTTTATGTCTTCGGTCATGCTGCCGTTGTTTAt   >  2:391131/1‑139 (MQ=255)
                                                                                                                                ggggATCTCACTGGCGTTAGTTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATCCTTGCGCGGCGTGCCGCTGATTACCGTTCTGTTTATGTCTTCGGTCATGCTGCCGTTGTTTATg  >  2:576234/1‑139 (MQ=255)
                                                                                                                                   gATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCg                                                  >  2:303643/1‑88 (MQ=255)
                                                                                                                                   gATCTCACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCg                                                  <  1:303643/88‑1 (MQ=255)
                                                                                                                                     tctcACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCg                                                                                   <  2:1196805/53‑1 (MQ=255)
                                                                                                                                     tctcACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCg                                                                                   >  1:1196805/1‑53 (MQ=255)
                                                                                                                                     tctcACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGgcg                                                            >  2:1307434/1‑76 (MQ=255)
                                                                                                                                     tctcACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGgcg                                                            <  1:1307434/76‑1 (MQ=255)
                                                                                                                                     tctcACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTACCg                                        <  2:1150940/96‑1 (MQ=255)
                                                                                                                                     tctcACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTACCg                                        >  1:1150940/1‑96 (MQ=255)
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CCACTGATTGTCTGGTGGCTGATGTATGGCGGTTTTTTTGCTCTTGAGCGGGTTGAAACCCGGCAATGGGGCGGGCTGACGCTAACTTTAATTATTGCATCAGTTGGGATTGCGGGGGCGCTACCGTGGGGGATCTTACTGGCGTTAGGTCGCCGCTCCCATATGCCGATTGTGCGTATCTTATCGGTCATTTTTATCGAATTCTGGCGCGGCGTGCCGCTGATTACCGTTCTGTTTATGTCTTCGGTCATGCTGCCGTTGTTTATG  >  NC_000913/3421724‑3421990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: