Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	340,089	G→A	intergenic (+346/‑76)	yahG → / → yahI	DUF1116 family protein/carbamate kinase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	340,089	0	G	A	100.0%	219.1 / NA	69	intergenic (+346/‑76)	yahG/yahI	DUF1116 family protein/carbamate kinase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/35);  total (34/35)

CGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATA  >  NC_000913/339954‑340216                                                                                                                                        |                                                                                                                                cgTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGcc                                                                                                                              >  2:264272/1‑139 (MQ=255)                      aCGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                         <  1:1114557/139‑1 (MQ=255)                             gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                         >  2:967141/1‑132 (MQ=255)                             gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                         <  1:967141/132‑1 (MQ=255)                             gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTg                                                                                                  >  2:914196/1‑139 (MQ=255)                             gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTg                                                                                                  >  1:783938/1‑139 (MQ=255)                                        tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCAt                                                                                       <  1:848830/139‑1 (MQ=255)                                               cTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                                >  2:917708/1‑139 (MQ=255)                                               cTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                                <  2:783938/139‑1 (MQ=255)                                                 gATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc                                                                              <  1:917708/139‑1 (MQ=255)                                                 gATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc                                                                              <  2:306334/139‑1 (MQ=255)                                                           gcgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCa                                                                                                       <  2:695810/104‑1 (MQ=255)                                                           gcgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCa                                                                                                       >  1:695810/1‑104 (MQ=255)                                                            cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc                                                                           <  1:376232/131‑1 (MQ=255)                                                            cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc                                                                           >  2:376232/1‑131 (MQ=255)                                                               aTCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCgg                                                                >  1:548389/1‑139 (MQ=255)                                                                   ggCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACAt                                                            <  2:548389/139‑1 (MQ=255)                                                                                  tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCa                                                                                                           >  2:885117/1‑77 (MQ=255)                                                                                  tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                          <  1:930607/126‑1 (MQ=255)                                                                                  tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                          >  2:930607/1‑126 (MQ=255)                                                                                  tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAagag                                             >  2:337045/1‑139 (MQ=255)                                                                                  tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCGGATAAGCGTAGCGCATCa                                                                                                           <  1:885117/77‑1 (MQ=255)                                                                                    ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                         >  2:750337/1‑77 (MQ=255)                                                                                    ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                         <  1:750337/77‑1 (MQ=255)                                                                                            ggcgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGc                                                                                                                               >  1:333386/1‑47 (MQ=255)                                                                                            ggcgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                                     >  1:979045/1‑89 (MQ=255)                                                                                            ggcgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                                     <  2:979045/89‑1 (MQ=255)                                                                                             gcgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                          >  2:1242308/1‑115 (MQ=255)                                                                                             gcgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                          <  1:1242308/115‑1 (MQ=255)                                                                                                  aaCGCCTTATCCGGCCGACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGATAGAGCTTGTGGTCGTTTCCa                             <  1:914196/139‑1 (MQ=255)                                                                                                       cTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATtggt                        <  1:383984/139‑1 (MQ=255)                                                                                                            ccGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                               >  2:703036/1‑63 (MQ=255)                                                                                                            ccGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                               <  1:703036/63‑1 (MQ=255)                                                                                                            ccGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTa                                                                    >  1:1342071/1‑90 (MQ=255)                                                                                                            ccGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTa                                                                    <  2:1342071/90‑1 (MQ=255)                                                                                                               gCCTACGAGTGGCGCGAGAATTGTAAGCCTGGTAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                          >  2:809539/1‑97 (MQ=255)                                                                                                               gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc                                                                              <  1:1195141/77‑1 (MQ=255)                                                                                                               gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc                                                                              >  2:1195141/1‑77 (MQ=255)                                                                                                               gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                          <  1:809539/97‑1 (MQ=255)                                                                                                               gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGt                                  >  1:847590/1‑121 (MQ=255)                                                                                                               gCCTACGAGTGGCGCGAGAATTATAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGt                                  <  2:847590/121‑1 (MQ=255)                                                                                                                ccTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                         >  2:868857/1‑49 (MQ=255)                                                                                                                ccTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                         <  1:868857/49‑1 (MQ=255)                                                                                                                ccTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTa                                                                    >  1:1268520/1‑86 (MQ=255)                                                                                                                ccTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTa                                                                    <  2:1268520/86‑1 (MQ=255)                                                                                                                ccTACGAGTGGCGCGAGAATCGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCaa                   >  2:154319/1‑135 (MQ=255)                                                                                                                ccTACGAGTGGCGCGAGAATCGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCaa                   <  1:154319/135‑1 (MQ=255)                                                                                                                 cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTa                                                                                                                   <  1:627983/38‑1 (MQ=255)                                                                                                                 cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTa                                                                                                                   >  2:627983/1‑38 (MQ=255)                                                                                                                 cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCa                                                                                                              >  1:465517/1‑43 (MQ=255)                                                                                                                 cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCa                                                                                                              <  2:465517/43‑1 (MQ=255)                                                                                                                 cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa              <  1:337045/139‑1 (MQ=255)                                                                                                                 cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa              <  2:416942/139‑1 (MQ=255)                                                                                                                      aGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATc         <  1:264272/139‑1 (MQ=255)                                                                                                                         ggCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAg                <  1:131146/129‑1 (MQ=255)                                                                                                                         ggCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAg                >  2:131146/1‑129 (MQ=255)                                                                                                                         ggCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa              <  1:1307026/131‑1 (MQ=255)                                                                                                                         ggCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa              >  2:1307026/1‑131 (MQ=255)                                                                                                                          gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTc                                    <  2:55775/108‑1 (MQ=255)                                                                                                                          gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTc                                    >  1:55775/1‑108 (MQ=255)                                                                                                                          gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAg     >  2:1320929/1‑139 (MQ=255)                                                                                                                          gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAg     >  1:1169859/1‑139 (MQ=255)                                                                                                                             cgAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATa  >  2:410953/1‑139 (MQ=255)                                                                                                                               agaATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                          <  1:1340842/81‑1 (MQ=255)                                                                                                                               agaATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                          >  2:1340842/1‑81 (MQ=255)                                                                                                                               agaATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCa                    >  2:806342/1‑119 (MQ=255)                                                                                                                               agaATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCa                    <  1:806342/119‑1 (MQ=255)                                                                                                                                    tGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCg                                   <  2:870341/99‑1 (MQ=255)                                                                                                                                    tGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCg                                   >  1:870341/1‑99 (MQ=255)                                                                                                                                        |                                                                                                                                CGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATA  >  NC_000913/339954‑340216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑