Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,516,104	T→C	K401K (AAA→AAG)	damX ←	cell division protein that binds to the septal ring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,516,104	0	T	C	100.0%	183.5 / NA	51	K401K (AAA→AAG)	damX	cell division protein that binds to the septal ring
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

GCGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTA  >  NC_000913/3515969‑3516239                                                                                                                                        |                                                                                                                                        gcgcTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCcttt                                                                                                                                      <  2:1295943/139‑1 (MQ=255)                 ccGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCg                                                                                                                      >  1:172362/1‑139 (MQ=255)                   gCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGaa                                                                                                                    <  2:172362/139‑1 (MQ=255)                         aTAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTac                                                                                                              >  1:875244/1‑139 (MQ=255)                                  tGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTc                                                                                                                       <  2:824795/121‑1 (MQ=255)                                  tGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCAGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTc                                                                                                                       >  1:824795/1‑121 (MQ=255)                                      ggAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTac                                                                                                              <  2:358870/126‑1 (MQ=255)                                      ggAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTac                                                                                                              >  1:358870/1‑126 (MQ=255)                                      ggAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCa                                                                                                 <  1:119238/139‑1 (MQ=255)                                        agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAgg                                                                                               <  2:875244/139‑1 (MQ=255)                                           tAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTa                                                                                                               <  1:1014620/120‑1 (MQ=255)                                           tAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTa                                                                                                               >  2:1014620/1‑120 (MQ=255)                                                   aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACgg                                                                                    <  1:447668/139‑1 (MQ=255)                                                   aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACgg                                                                                    <  2:760643/139‑1 (MQ=255)                                                   aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACgg                                                                                    <  1:1070466/139‑1 (MQ=255)                                                             aTCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCa                                                                                       >  2:1222047/1‑126 (MQ=255)                                                             aTCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCa                                                                                       <  1:1222047/126‑1 (MQ=255)                                                                 gCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                                      >  1:49886/1‑139 (MQ=255)                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTac                                                                                                              >  1:80818/1‑98 (MQ=255)                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTac                                                                                                              <  2:80818/98‑1 (MQ=255)                                                                   cTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                    <  2:748679/139‑1 (MQ=255)                                                                           gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                    <  1:687401/131‑1 (MQ=255)                                                                           gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                    >  2:687401/1‑131 (MQ=255)                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAg                                                           >  1:61535/1‑139 (MQ=255)                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCa                                                                                       >  1:145189/1‑107 (MQ=255)                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCa                                                                                       <  2:145189/107‑1 (MQ=255)                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCAtt                                                                           >  1:1340883/1‑119 (MQ=255)                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCAtt                                                                           <  2:1340883/119‑1 (MQ=255)                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACaa                                                       >  2:825903/1‑139 (MQ=255)                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTACTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                        >  2:942354/1‑90 (MQ=255)                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTACTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                        <  1:942354/90‑1 (MQ=255)                                                                                  cGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACg                                                     >  2:31689/1‑139 (MQ=255)                                                                                       tCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaaa                                                                                                    <  2:958654/87‑1 (MQ=255)                                                                                       tCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaaa                                                                                                    >  1:958654/1‑87 (MQ=255)                                                                                          cccACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcg                                                                        <  2:1263592/112‑1 (MQ=255)                                                                                          cccACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcg                                                                        >  1:1263592/1‑112 (MQ=255)                                                                                                         ccTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCtctttctt                              >  2:1269714/1‑139 (MQ=255)                                                                                                                       ggCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGtt                  <  2:1085247/137‑1 (MQ=255)                                                                                                                       ggCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGtt                  >  1:1085247/1‑137 (MQ=255)                                                                                                                        gCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGcc                                                                                                         <  2:783595/49‑1 (MQ=255)                                                                                                                        gCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGcc                                                                                                         >  1:783595/1‑49 (MQ=255)                                                                                                                         cAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAgg              <  1:31689/139‑1 (MQ=255)                                                                                                                         cAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAgg              <  1:1269714/139‑1 (MQ=255)                                                                                                                            tGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGa                                         <  1:1222830/109‑1 (MQ=255)                                                                                                                            tGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGa                                         >  2:1222830/1‑109 (MQ=255)                                                                                                                            tGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCaa                       <  2:193027/127‑1 (MQ=255)                                                                                                                            tGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCaa                       >  1:193027/1‑127 (MQ=255)                                                                                                                                   aCCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGt          <  2:351957/133‑1 (MQ=255)                                                                                                                                   aCCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGt          >  1:351957/1‑133 (MQ=255)                                                                                                                                    ccGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGtt   >  1:1052211/1‑139 (MQ=255)                                                                                                                                     cGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTa  >  2:866254/1‑139 (MQ=255)                                                                                                                                     cGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTa  >  2:1008899/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        GCGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTA  >  NC_000913/3515969‑3516239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑