Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,731,980 G→A G284S (GGT→AGT)  xylF → D‑xylose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,731,9800GA100.0% 264.6 / NA 82G284S (GGT→AGT) xylFD‑xylose transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/40);  total (42/40)

GTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAG  >  NC_000913/3731845‑3732113
                                                                                                                                       |                                                                                                                                     
gTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCGATAGTc                                                                                                                                    >  1:1173510/1‑139 (MQ=255)
       tctcCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAAcc                                                                                                                             >  1:719222/1‑139 (MQ=255)
           cGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaaaa                                                                                                                         >  1:319842/1‑139 (MQ=255)
             gCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                       <  1:154418/139‑1 (MQ=255)
                  gATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                  >  1:181997/1‑139 (MQ=255)
                     gCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                               <  2:181997/139‑1 (MQ=255)
                       ggATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAg                                                                                                                                  >  2:290918/1‑118 (MQ=255)
                       ggATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAg                                                                                                                                  <  1:290918/118‑1 (MQ=255)
                            tcGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                           >  1:848043/1‑120 (MQ=255)
                            tcGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                           <  2:848043/120‑1 (MQ=255)
                               cAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACcaca                                                                                                             <  1:634293/131‑1 (MQ=255)
                               cAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACcaca                                                                                                             >  2:634293/1‑131 (MQ=255)
                                aGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataat                                                                                                    <  1:720766/139‑1 (MQ=255)
                                        aaCGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                           >  2:598760/1‑108 (MQ=255)
                                        aaCGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                           <  1:598760/108‑1 (MQ=255)
                                        aaCGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                  >  2:1099186/1‑117 (MQ=255)
                                        aaCGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                  <  1:1099186/117‑1 (MQ=255)
                                            tATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                       >  1:1326754/1‑108 (MQ=255)
                                            tATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                       <  2:1326754/108‑1 (MQ=255)
                                             aTTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                       <  2:1083846/107‑1 (MQ=255)
                                             aTTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                       >  1:1083846/1‑107 (MQ=255)
                                             aTTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATg                                                                                       >  2:501341/1‑139 (MQ=255)
                                                   gcCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCt                                                                                 <  2:319842/139‑1 (MQ=255)
                                                    ccGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCa                                                                                                                            <  2:1086183/95‑1 (MQ=255)
                                                    ccGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCa                                                                                                                            >  1:1086183/1‑95 (MQ=255)
                                                       gTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                             <  1:501341/139‑1 (MQ=255)
                                                                cTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGac                                                                    <  2:1260328/139‑1 (MQ=255)
                                                                  aTGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACcaca                                                                                                             <  1:399339/96‑1 (MQ=255)
                                                                  aTGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACcaca                                                                                                             >  2:399339/1‑96 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                           <  2:327621/75‑1 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                           >  1:327621/1‑75 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTccc                                                                                   <  1:688768/115‑1 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTccc                                                                                   >  2:688768/1‑115 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcgatcga                                                           >  1:1135602/1‑139 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcgat                                                              >  1:381568/1‑136 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcgat                                                              <  2:381568/136‑1 (MQ=255)
                                                                         tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTAAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGACTCCTGACACcgatcga                                                           >  2:762495/1‑139 (MQ=255)
                                                                           tataAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAGTTGCCGTTGAGTTGGGCAAGAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                             >  2:888562/1‑119 (MQ=255)
                                                                           tataAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                  >  2:78051/1‑82 (MQ=255)
                                                                           tataAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                                  <  1:78051/82‑1 (MQ=255)
                                                                           tataAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                             <  1:888562/119‑1 (MQ=255)
                                                                           tataAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                         >  1:20407/1‑139 (MQ=255)
                                                                           tataAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                         <  2:719222/139‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATGCTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCa                                                                                                                                   >  1:325824/1‑55 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATGCTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCa                                                                                                                                   <  2:325824/55‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                           >  1:906220/1‑63 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                           <  2:906220/63‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                       >  1:1155969/1‑67 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                       <  2:1155969/67‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                    <  1:680950/70‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                    >  2:680950/1‑70 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATAcc                                                                                                                >  1:1150228/1‑74 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATAcc                                                                                                                <  2:1150228/74‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGc                                                                            >  1:1230375/1‑110 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGc                                                                            <  2:1230375/110‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                         <  1:1324657/129‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                               >  2:56332/1‑139 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                               <  1:56332/139‑1 (MQ=255)
                                                                                     ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGAAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                         >  2:1324657/1‑129 (MQ=255)
                                                                                           tgttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACAt                                         >  2:679534/1‑139 (MQ=255)
                                                                                            gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATc                                        >  2:137807/1‑139 (MQ=255)
                                                                                            gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATc                                        >  2:1123393/1‑139 (MQ=255)
                                                                                               ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGa                                                                                         >  2:818837/1‑87 (MQ=255)
                                                                                               ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGa                                                                                         <  1:818837/87‑1 (MQ=255)
                                                                                                  aaaTACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGAt                                  >  1:1271991/1‑139 (MQ=255)
                                                                                                     tACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACg                               <  2:1271991/139‑1 (MQ=255)
                                                                                                       cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                       >  1:163955/1‑49 (MQ=255)
                                                                                                       cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                       <  2:163955/49‑1 (MQ=255)
                                                                                                       cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAg                                                                                                                     >  1:52477/1‑51 (MQ=255)
                                                                                                       cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAg                                                                                                                     <  2:52477/51‑1 (MQ=255)
                                                                                                       cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACcaca                                                                                                             <  2:648064/59‑1 (MQ=255)
                                                                                                       cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACcaca                                                                                                             >  1:648064/1‑59 (MQ=255)
                                                                                                       cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGt                             <  1:679534/139‑1 (MQ=255)
                                                                                                       cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGt                             >  2:915795/1‑139 (MQ=255)
                                                                                                          ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAAt                          >  2:351673/1‑139 (MQ=255)
                                                                                                             cAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                             <  2:265855/85‑1 (MQ=255)
                                                                                                             cAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                             >  1:265855/1‑85 (MQ=255)
                                                                                                                     gCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGAt               <  2:20407/139‑1 (MQ=255)
                                                                                                                              ttGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAag      <  1:351673/139‑1 (MQ=255)
                                                                                                                                 ggCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCca           >  1:1159105/1‑131 (MQ=255)
                                                                                                                                 ggCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCca           <  2:1159105/131‑1 (MQ=255)
                                                                                                                                  gCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAagagag  <  1:1123393/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAG  >  NC_000913/3731845‑3732113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑