Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,937,168	G→A	*297* (TAG→TAA)	rbsB →	D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,937,168	0	G	A	100.0%	209.6 / NA	65	*297* (TAG→TAA)	rbsB	D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/36);  total (29/36)

GCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCC  >  NC_000913/3937032‑3937288                                                                                                                                         |                                                                                                                         gCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAAtt                                                                                                                        <  1:675176/139‑1 (MQ=255)      cTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAAt                                                                                                                   <  2:598568/139‑1 (MQ=255)        agcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCa                                                                                                                 <  1:104538/139‑1 (MQ=255)         gcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                <  2:475032/139‑1 (MQ=255)              gACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGt                                                                                                           <  2:203049/139‑1 (MQ=255)               aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                          >  1:664885/1‑139 (MQ=255)               aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                          <  1:366980/139‑1 (MQ=255)               aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                          >  2:366980/1‑139 (MQ=255)               aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGCTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                          >  2:322282/1‑139 (MQ=255)                 tATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATg                                                                                                        >  1:442145/1‑139 (MQ=255)                   tCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGAc                                                                                                      <  2:1119885/139‑1 (MQ=255)                            tACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTg                                                                                             <  2:442145/139‑1 (MQ=255)                             aCCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGa                                                                                            >  1:375652/1‑139 (MQ=255)                              cccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGAc                                                                                           >  2:454005/1‑139 (MQ=255)                              cccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGAc                                                                                           <  1:1124544/139‑1 (MQ=255)                                cGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGt                                                                                              <  2:467916/134‑1 (MQ=255)                                cGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGt                                                                                              >  1:467916/1‑134 (MQ=255)                                         tGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTc                                                                                <  1:454005/139‑1 (MQ=255)                                            cgcgAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGt                                                                                              >  2:1146514/1‑122 (MQ=255)                                            cgcgAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGt                                                                                              <  1:1146514/122‑1 (MQ=255)                                                aaaGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGTTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGa                                                                           <  1:98970/137‑1 (MQ=255)                                                aaaGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGTTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGa                                                                           >  2:98970/1‑137 (MQ=255)                                                     cGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                    <  2:664885/139‑1 (MQ=255)                                                       tCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAAc                                                                  <  1:322282/139‑1 (MQ=255)                                                          aaaCCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                               <  1:258531/139‑1 (MQ=255)                                                              cGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGt                                                                                              <  2:994828/104‑1 (MQ=255)                                                              cGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGt                                                                                              >  1:994828/1‑104 (MQ=255)                                                                  gATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGAc                                                                                                      <  1:427423/92‑1 (MQ=255)                                                                  gATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGAc                                                                                                      >  2:427423/1‑92 (MQ=255)                                                                     aaaGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGa                                                    >  1:41857/1‑139 (MQ=255)                                                                          gCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAAc                                                                  <  1:1050504/120‑1 (MQ=255)                                                                          gCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAAc                                                                  >  2:1050504/1‑120 (MQ=255)                                                                              aaaGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCa                                                                                                                 >  1:972586/1‑69 (MQ=255)                                                                              aaaGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCa                                                                                                                 <  2:972586/69‑1 (MQ=255)                                                                                aGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                    <  2:715734/112‑1 (MQ=255)                                                                                aGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCa                                         <  2:375652/139‑1 (MQ=255)                                                                                aGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATCGTGACATAAATACGTCATCGACAGATGa                                                                    >  1:715734/1‑112 (MQ=255)                                                                                 ggCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAAt                                                                                                                   >  2:106852/1‑64 (MQ=255)                                                                                 ggCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAAt                                                                                                                   <  1:106852/64‑1 (MQ=255)                                                                                     agaAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                    <  1:620651/139‑1 (MQ=255)                                                                                                    aGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                               <  1:179797/97‑1 (MQ=255)                                                                                                    aGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                               >  2:179797/1‑97 (MQ=255)                                                                                                     gTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGa                                                    <  1:392488/107‑1 (MQ=255)                                                                                                     gTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGa                                                    >  2:392488/1‑107 (MQ=255)                                                                                                              tGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTa           >  1:383804/1‑139 (MQ=255)                                                                                                               gATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAAt                                                                                     >  2:236828/1‑64 (MQ=255)                                                                                                               gATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAAt                                                                                     <  1:236828/64‑1 (MQ=255)                                                                                                                aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                >  1:1149561/1‑36 (MQ=255)                                                                                                                aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                <  2:1149561/36‑1 (MQ=255)                                                                                                                aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAt                                                                              <  2:1099047/70‑1 (MQ=255)                                                                                                                aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAt                                                                              >  1:1099047/1‑70 (MQ=255)                                                                                                                aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                    <  2:1175516/112‑1 (MQ=255)                                                                                                                aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                    >  1:1175516/1‑112 (MQ=255)                                                                                                                aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATg         >  1:382142/1‑139 (MQ=255)                                                                                                                       aCTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATcc  <  2:41857/139‑1 (MQ=255)                                                                                                                           gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATgg                                                                                               >  1:1180342/1‑42 (MQ=255)                                                                                                                           gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATgg                                                                                               <  2:1180342/42‑1 (MQ=255)                                                                                                                           gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCAcgcg                                     <  1:1219340/100‑1 (MQ=255)                                                                                                                           gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCAcgcg                                     >  2:1219340/1‑100 (MQ=255)                                                                                                                           gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                    <  1:62523/101‑1 (MQ=255)                                                                                                                           gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                    >  2:62523/1‑101 (MQ=255)                                                                                                                            ttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGa                                                                           >  2:181652/1‑61 (MQ=255)                                                                                                                            ttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGa                                                                           <  1:181652/61‑1 (MQ=255)                                                                                                                             tgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATgg        >  2:1017052/1‑127 (MQ=255)                                                                                                                             tgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATgg        <  1:1017052/127‑1 (MQ=255)                                                                                                                                         |                                                                                                                         GCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCC  >  NC_000913/3937032‑3937288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑