Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,992,054	C→T	P301L (CCA→CTA)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,992,054	0	C	T	100.0%	188.8 / NA	59	P301L (CCA→CTA)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/31);  total (28/31)

TCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCG  >  NC_000913/3991921‑3992176                                                                                                                                      |                                                                                                                           tCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACt                                                                                                                       >  2:434667/1‑139 (MQ=255)    ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                    <  1:434667/139‑1 (MQ=255)      aTACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCatg                                                                                                                  >  1:497186/1‑139 (MQ=255)            aGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                            >  2:945450/1‑139 (MQ=255)            aGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                            >  1:641452/1‑139 (MQ=255)            aGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                            >  2:545441/1‑139 (MQ=255)             gCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTgg                                                                                                           >  2:473273/1‑139 (MQ=255)              cGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                          <  2:716982/139‑1 (MQ=255)              cGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                          <  2:497186/139‑1 (MQ=255)                 tACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCg                                                                                                       <  2:1303823/139‑1 (MQ=255)                  aCTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                    <  2:483283/125‑1 (MQ=255)                  aCTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                    >  1:483283/1‑125 (MQ=255)                   cTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                     <  1:210386/139‑1 (MQ=255)                   cTGAAAACACTGCTGCTGGAAGCCTAGTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                     <  2:855949/139‑1 (MQ=255)                           acTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGaa                                                                                             >  2:1166116/1‑139 (MQ=255)                              gctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                    <  1:825557/113‑1 (MQ=255)                              gctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                    >  2:825557/1‑113 (MQ=255)                                      aaGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGc                                                                                  <  2:641452/139‑1 (MQ=255)                                         ccTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTAt                                                                                                                          <  2:40608/96‑1 (MQ=255)                                         ccTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTAt                                                                                                                          >  1:40608/1‑96 (MQ=255)                                             ttCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGaa                                                                           >  1:688848/1‑139 (MQ=255)                                                    gAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTa                                                                    >  1:1044358/1‑139 (MQ=255)                                                    gAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTa                                                                    <  1:1166116/139‑1 (MQ=255)                                                      aTACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAcc                                                                  <  2:489520/139‑1 (MQ=255)                                                       tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                    >  1:487609/1‑88 (MQ=255)                                                       tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                    <  2:487609/88‑1 (MQ=255)                                                       tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAccc                                                                 >  2:858214/1‑139 (MQ=255)                                                         cccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGt                                                               >  1:1270820/1‑139 (MQ=255)                                                          ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTa                                                                                                  <  1:1032264/103‑1 (MQ=255)                                                          ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTa                                                                                                  >  2:1032264/1‑103 (MQ=255)                                                          ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                              <  1:945450/139‑1 (MQ=255)                                                          ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                              >  1:224588/1‑139 (MQ=255)                                                                ccACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGAtt                                                        <  2:1270820/139‑1 (MQ=255)                                                                       tgctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                          >  1:745318/1‑82 (MQ=255)                                                                       tgctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                          <  2:745318/82‑1 (MQ=255)                                                                         ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGc                                                                                  >  1:124062/1‑104 (MQ=255)                                                                         ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGc                                                                                  <  2:124062/104‑1 (MQ=255)                                                                            gCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCgct                                            <  2:1044358/139‑1 (MQ=255)                                                                                aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                        <  2:224588/139‑1 (MQ=255)                                                                                aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                        <  2:688848/139‑1 (MQ=255)                                                                                 aGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAcc                                                                  <  2:423532/112‑1 (MQ=255)                                                                                 aGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAcc                                                                  >  1:423532/1‑112 (MQ=255)                                                                                      tCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                     <  2:334522/72‑1 (MQ=255)                                                                                      tCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                     >  1:334522/1‑72 (MQ=255)                                                                                             gCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                    <  1:820686/50‑1 (MQ=255)                                                                                             gCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                    >  2:820686/1‑50 (MQ=255)                                                                                              cGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAgtgt                          <  1:858214/139‑1 (MQ=255)                                                                                               gTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAcc                                                                  >  2:368236/1‑98 (MQ=255)                                                                                               gTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAcc                                                                  <  1:368236/98‑1 (MQ=255)                                                                                                tttGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGc                        >  2:285467/1‑139 (MQ=255)                                                                                                   gCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGaa                     <  1:285467/139‑1 (MQ=255)                                                                                                      cgacgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTg                                                                             >  2:433399/1‑80 (MQ=255)                                                                                                      cgacgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTg                                                                             <  1:433399/80‑1 (MQ=255)                                                                                                            cGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGc                                                                                                        <  2:569705/47‑1 (MQ=255)                                                                                                            cGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGc                                                                                                        >  1:569705/1‑47 (MQ=255)                                                                                                             gagaTTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATGCCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGcc           <  2:376914/139‑1 (MQ=255)                                                                                                                      tCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAAcgcg  >  2:635324/1‑139 (MQ=255)                                                                                                                              tctcGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                        <  1:156992/93‑1 (MQ=255)                                                                                                                              tctcGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                        >  2:156992/1‑93 (MQ=255)                                                                                                                                      |                                                                                                                           TCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCG  >  NC_000913/3991921‑3992176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑