Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,225,294	A→G	G489G (GGA→GGG)	metH →	homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,225,294	0	A	G	100.0%	168.6 / NA	48	G489G (GGA→GGG)	metH	homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/19);  total (29/19)

GGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAA  >  NC_000913/4225175‑4225426                                                                                                                        |                                                                                                                                     ggCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCAc                                                                                                                   >  2:861890/1‑139 (MQ=255)  gCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACg                                                                                                                  >  1:741336/1‑139 (MQ=255)                      aTCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTc                                                                                              >  2:923711/1‑139 (MQ=255)                      aTCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTc                                                                                              >  2:582813/1‑139 (MQ=255)                        ctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCgg                                                                                            >  2:140172/1‑139 (MQ=255)                            aTGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGt                                                                                        >  1:45023/1‑139 (MQ=255)                                 agagGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaa                                                                                   >  2:369880/1‑139 (MQ=255)                                      gCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCgag                                                                                                        >  2:959536/1‑113 (MQ=255)                                      gCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCgag                                                                                                        <  1:959536/113‑1 (MQ=255)                                         tCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCAc                                                                           >  2:207117/1‑139 (MQ=255)                                               ccTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaa                                                                                   >  2:441501/1‑125 (MQ=255)                                               ccTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaa                                                                                   <  1:441501/125‑1 (MQ=255)                                               ccTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa                                                                            <  2:646031/132‑1 (MQ=255)                                               ccTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa                                                                            >  1:646031/1‑132 (MQ=255)                                                   tATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGtt                                                                 >  2:1257850/1‑139 (MQ=255)                                                         tCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTc                                                           >  2:765358/1‑139 (MQ=255)                                                          cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc                                                          <  1:765358/139‑1 (MQ=255)                                                          cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc                                                          <  1:861890/139‑1 (MQ=255)                                                          cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc                                                          >  2:1038799/1‑139 (MQ=255)                                                          cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGACCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc                                                          >  1:350245/1‑139 (MQ=255)                                                            cgcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt                                                                                               <  1:1222360/100‑1 (MQ=255)                                                            cgcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt                                                                                               >  2:1222360/1‑100 (MQ=255)                                                               gAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCa                                                     <  1:582813/139‑1 (MQ=255)                                                                                 cGGGGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGAc                                   <  2:970422/139‑1 (MQ=255)                                                                                      cGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc                                                                                          <  2:11311/79‑1 (MQ=255)                                                                                      cGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc                                                                                          >  1:11311/1‑79 (MQ=255)                                                                                        gCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGAc                                   <  1:1306053/132‑1 (MQ=255)                                                                                        gCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGAc                                   >  2:1306053/1‑132 (MQ=255)                                                                                         cAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACAt                           >  2:813508/1‑139 (MQ=255)                                                                                            tggtggTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc                                                                                          <  2:536560/73‑1 (MQ=255)                                                                                            tggtggTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc                                                                                          >  1:536560/1‑73 (MQ=255)                                                                                              gtggtAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGaa                                                                       <  1:209690/90‑1 (MQ=255)                                                                                              gtggtAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGaa                                                                       >  2:209690/1‑90 (MQ=255)                                                                                              gtggtAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCtt                                                            >  2:446741/1‑101 (MQ=255)                                                                                              gtggtAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCtt                                                            <  1:446741/101‑1 (MQ=255)                                                                                                      ggCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGc                                                              >  1:669140/1‑91 (MQ=255)                                                                                                      ggCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGc                                                              <  2:669140/91‑1 (MQ=255)                                                                                                      ggCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCa              <  1:813508/139‑1 (MQ=255)                                                                                                        ccTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGa                                                                        <  1:567864/79‑1 (MQ=255)                                                                                                        ccTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGa                                                                        >  2:567864/1‑79 (MQ=255)                                                                                                        ccTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCtt                                       >  1:562519/1‑112 (MQ=255)                                                                                                        ccTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCtt                                       <  2:562519/112‑1 (MQ=255)                                                                                                           ttGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTgg         >  2:1032813/1‑139 (MQ=255)                                                                                                            tGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGTTATTTGCCGTCTGGCGTACCAAATCCTCACCGAAGAGGTTGGCTACCCCCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGc        >  2:481757/1‑139 (MQ=255)                                                                                                                 aaCAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCgg                                                                                            <  1:994827/50‑1 (MQ=255)                                                                                                                 aaCAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCgg                                                                                            >  2:994827/1‑50 (MQ=255)                                                                                                                 aaCAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGa   >  2:1286688/1‑139 (MQ=255)                                                                                                                  aCAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGaa  <  1:1286688/139‑1 (MQ=255)                                                                                                                        |                                                                                                                                     GGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAA  >  NC_000913/4225175‑4225426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑