Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,280,033	G→A	G18G (GGG→GGA)	yjcE →	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,280,033	0	G	A	100.0%	227.9 / NA	72	G18G (GGG→GGA)	yjcE	putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/34);  total (38/34)

TTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTG  >  NC_000913/4279900‑4280159                                                                                                                                      |                                                                                                                               tttGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGagtggt                                                                                                                           <  2:1180596/139‑1 (MQ=255) tttGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGagtggt                                                                                                                           >  1:1180596/1‑139 (MQ=255)   tGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCa                                                                                                                         >  2:837273/1‑139 (MQ=255)   ggCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCa                                                                                                                         >  2:842009/2‑139 (MQ=255)     cGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACt                                                                                                                       <  1:837273/139‑1 (MQ=255)      gATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTc                                                                                                                      <  1:813775/139‑1 (MQ=255)                         gaATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGAt                                                                                                   >  1:59467/1‑139 (MQ=255)                          aaTTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTTTCATGCCCTTTCAGATc                                                                                                  <  1:842009/139‑1 (MQ=255)                                       cGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCccgct                                                                                             <  2:388436/131‑1 (MQ=255)                                       cGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCccgct                                                                                             >  1:388436/1‑131 (MQ=255)                                               tcAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATc                                                                             >  2:17344/1‑139 (MQ=255)                                                   aTCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCccgcttccg                                                                                         <  2:97033/123‑1 (MQ=255)                                                   aTCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCccgcttccg                                                                                         >  1:97033/1‑123 (MQ=255)                                                   aTCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCa                                                                         >  2:667770/1‑139 (MQ=255)                                                     cATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATc                                                                       <  1:399297/139‑1 (MQ=255)                                                      aTAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTTAGATCCCGCTTCCGCTTATGCAAATCGCCATct                                                                      >  2:1168653/1‑138 (MQ=255)                                                         aaaaaCATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                   <  1:379218/139‑1 (MQ=255)                                                         aaaaaCATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                   <  2:1268741/139‑1 (MQ=255)                                                             aCATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCAt                                                                        >  1:189538/1‑130 (MQ=255)                                                             aCATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCAt                                                                        <  2:189538/130‑1 (MQ=255)                                                               aTCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCaa                                                                                <  2:208299/120‑1 (MQ=255)                                                               aTCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCaa                                                                                >  1:208299/1‑120 (MQ=255)                                                               aTCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTAc                                                             >  1:252223/1‑139 (MQ=255)                                                                tCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACt                                                                                                                       <  2:961356/80‑1 (MQ=255)                                                                tCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACt                                                                                                                       >  1:961356/1‑80 (MQ=255)                                                                 cGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATg                                                                                                              >  2:239180/1‑88 (MQ=255)                                                                 cGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATg                                                                                                              <  1:239180/88‑1 (MQ=255)                                                                 cGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                   >  2:1287541/1‑131 (MQ=255)                                                                 cGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                   <  1:1287541/131‑1 (MQ=255)                                                                          ggAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                   >  2:287099/1‑122 (MQ=255)                                                                          ggAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                   <  1:287099/122‑1 (MQ=255)                                                                                cATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACgttt                                            >  2:613532/1‑139 (MQ=255)                                                                                          ttcttcACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCAtg                                  <  1:667770/139‑1 (MQ=255)                                                                                             ttcACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                   <  2:330822/103‑1 (MQ=255)                                                                                             ttcACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                   >  1:330822/1‑103 (MQ=255)                                                                                               cACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCa                                                                                                                >  1:1197206/1‑56 (MQ=255)                                                                                               cACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCa                                                                                                                <  2:1197206/56‑1 (MQ=255)                                                                                               cACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACgtt                                             <  2:507784/123‑1 (MQ=255)                                                                                               cACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACgtt                                             >  1:507784/1‑123 (MQ=255)                                                                                               cACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACg                                               >  2:65962/1‑121 (MQ=255)                                                                                               cACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACg                                               <  1:65962/121‑1 (MQ=255)                                                                                                 ccATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGtt                           <  2:59467/139‑1 (MQ=255)                                                                                                     aCTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGAt                       >  2:693296/1‑139 (MQ=255)                                                                                                         aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACgttt                                            <  2:394069/114‑1 (MQ=255)                                                                                                         aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACgttt                                            >  1:394069/1‑114 (MQ=255)                                                                                                         aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGtt                           <  2:1209339/131‑1 (MQ=255)                                                                                                         aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGtt                           >  1:1209339/1‑131 (MQ=255)                                                                                                         aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTg                   >  2:1120000/1‑139 (MQ=255)                                                                                                            aTGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCAt                                                                                                               <  1:193291/44‑1 (MQ=255)                                                                                                            aTGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCAt                                                                                                               >  2:193291/1‑44 (MQ=255)                                                                                                              gACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAActc              >  1:881963/1‑139 (MQ=255)                                                                                                              gACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAActc              >  2:1286462/1‑139 (MQ=255)                                                                                                               aCCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTTAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAAc                <  1:855692/136‑1 (MQ=255)                                                                                                               aCCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAAc                >  2:855692/1‑136 (MQ=255)                                                                                                                cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCttt                                                                                                        <  1:604588/47‑1 (MQ=255)                                                                                                                cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCttt                                                                                                        >  2:604588/1‑47 (MQ=255)                                                                                                                cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                   <  1:1165342/84‑1 (MQ=255)                                                                                                                cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                   >  2:1165342/1‑84 (MQ=255)                                                                                                                cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCtt            >  2:10277/1‑139 (MQ=255)                                                                                                                cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCtt            >  2:824374/1‑139 (MQ=255)                                                                                                                cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCtt            >  1:566715/1‑139 (MQ=255)                                                                                                                  cTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAGCTCtttt          <  2:881963/139‑1 (MQ=255)                                                                                                                  cTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCtttt          <  1:1120000/139‑1 (MQ=255)                                                                                                                   tCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCa                                                                                                      <  2:1187082/46‑1 (MQ=255)                                                                                                                   tCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCa                                                                                                      >  1:1187082/1‑46 (MQ=255)                                                                                                                   tCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCgg                                                                     <  2:520914/79‑1 (MQ=255)                                                                                                                   tCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCgg                                                                     >  1:520914/1‑79 (MQ=255)                                                                                                                   tCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTgcg                                                                 >  2:1049043/1‑83 (MQ=255)                                                                                                                   tCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTgcg                                                                 <  1:1049043/83‑1 (MQ=255)                                                                                                                      tGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGt      >  2:1005522/1‑139 (MQ=255)                                                                                                                      tGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGt      <  1:693296/139‑1 (MQ=255)                                                                                                                          ctcGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTg  <  1:824374/139‑1 (MQ=255)                                                                                                                                   cGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCAtg                                  <  2:1168750/98‑1 (MQ=255)                                                                                                                                   cGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCAtg                                  >  1:1168750/1‑98 (MQ=255)                                                                                                                                      |                                                                                                                               TTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTG  >  NC_000913/4279900‑4280159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑