Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,324,876 A→G L97P (CTG→CCG)  phnC ← phosphonate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,324,8760AG100.0% 98.3 / NA 28L97P (CTG→CCG) phnCphosphonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/15);  total (13/15)

GCCAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCAAA  >  NC_000913/4324743‑4325010
                                                                                                                                     |                                                                                                                                      
gCCAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTg                                                                                                                                   <  1:79736/139‑1 (MQ=255)
  cAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGaa                                                                                                                                 <  1:377177/139‑1 (MQ=255)
  cAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGaa                                                                                                                                 <  1:783622/139‑1 (MQ=255)
          gTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGa                                                                                                                         >  2:585379/1‑139 (MQ=255)
               cgcCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATg                                                                                                                    <  1:585379/139‑1 (MQ=255)
                            cgcTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAata                                                                                                                      <  2:75536/124‑1 (MQ=255)
                            cgcTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAata                                                                                                                      >  1:75536/1‑124 (MQ=255)
                                                               ggTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCt                                                                    <  1:1012175/139‑1 (MQ=255)
                                                                gTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTa                                                                                                                  >  2:1254456/1‑92 (MQ=255)
                                                                gTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTa                                                                                                                  <  1:1254456/92‑1 (MQ=255)
                                                                                        aGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGc                                                                                                                >  2:439220/1‑70 (MQ=255)
                                                                                        aGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGc                                                                                                                <  1:439220/70‑1 (MQ=255)
                                                                                          cgcgcCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttg                                                                                                                              >  2:1218123/1‑54 (MQ=255)
                                                                                          cgcgcCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttg                                                                                                                              <  1:1218123/54‑1 (MQ=255)
                                                                                                         gTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGaa                                                                                                                                 >  1:654410/1‑36 (MQ=255)
                                                                                                         gTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGaa                                                                                                                                 <  2:654410/36‑1 (MQ=255)
                                                                                                            ctcCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCgg                                                                                         <  1:667800/73‑1 (MQ=255)
                                                                                                            ctcCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCgg                                                                                         >  2:667800/1‑73 (MQ=255)
                                                                                                                    cGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGt                                                                                                            >  1:594459/1‑46 (MQ=255)
                                                                                                                    cGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGt                                                                                                            <  2:594459/46‑1 (MQ=255)
                                                                                                                       tCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGc                               <  1:1075096/120‑1 (MQ=255)
                                                                                                                       tCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGc                               >  2:1075096/1‑120 (MQ=255)
                                                                                                                       tCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCg              <  2:205622/137‑1 (MQ=255)
                                                                                                                       tCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCg              >  1:205622/1‑137 (MQ=255)
                                                                                                                         aGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGata                                   <  1:892617/114‑1 (MQ=255)
                                                                                                                         aGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGata                                   >  2:892617/1‑114 (MQ=255)
                                                                                                                                 cACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCaaa  >  1:418971/1‑139 (MQ=255)
                                                                                                                                 cACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCaaa  >  1:248881/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GCCAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCAAA  >  NC_000913/4324743‑4325010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: