Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,331,634	C→T	A378V (GCG→GTG)	proP →	proline/glycine betaine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,331,634	0	C	T	100.0%	171.7 / NA	54	A378V (GCG→GTG)	proP	proline/glycine betaine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/25);  total (29/25)

CCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAT  >  NC_000913/4331502‑4331767                                                                                                                                     |                                                                                                                                      cccTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGtt                                                                                                                                 >  1:1205737/1‑139 (MQ=255)   cTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTcc                                                                                                                               <  2:493623/139‑1 (MQ=255)         gtgtTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                         <  1:243344/139‑1 (MQ=255)            ttGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGGTTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGCCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCtta                                                                                                                      >  2:740145/1‑139 (MQ=255)                          gTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTccc                                                                                                                              <  1:374768/117‑1 (MQ=255)                          gTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTccc                                                                                                                              >  2:374768/1‑117 (MQ=255)                             tATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGa                                                                                                                                     >  1:60771/1‑107 (MQ=255)                              aTTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTgg                                                                                                    >  2:210082/1‑139 (MQ=255)                                            tAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAat                                                                                      >  1:267042/1‑139 (MQ=255)                                              aCGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATc                                                                                                                    <  1:439887/107‑1 (MQ=255)                                              aCGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATc                                                                                                                    >  2:439887/1‑107 (MQ=255)                                                        ccTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                         >  1:864547/1‑92 (MQ=255)                                                        ccTGATTTTTGCCGGGCTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                         <  2:864547/92‑1 (MQ=255)                                                            aTTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                         <  2:561940/88‑1 (MQ=255)                                                            aTTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                         >  1:561940/1‑88 (MQ=255)                                                                      ggTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTa                                                                                                               >  1:257638/1‑88 (MQ=255)                                                                      ggTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTa                                                                                                               <  2:257638/88‑1 (MQ=255)                                                                      ggTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGAc                                                            >  2:1217053/1‑139 (MQ=255)                                                                                gCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTg                                                  >  1:395290/1‑139 (MQ=255)                                                                                   ggCGGTGATCCTTAACTGCTTTACGGGCGTCATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTggcg                                               >  2:495981/1‑139 (MQ=255)                                                                                    gCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                         <  2:586412/64‑1 (MQ=255)                                                                                    gCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                         >  1:586412/1‑64 (MQ=255)                                                                                        ttaTCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTcc                                                                                                                               <  2:111492/52‑1 (MQ=255)                                                                                        tGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTcc                                                                                                                               >  1:111492/1‑54 (MQ=255)                                                                                        tGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCctg                                          >  1:1114378/1‑139 (MQ=255)                                                                                          aTCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCt                                                                           <  1:710396/104‑1 (MQ=255)                                                                                          aTCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCt                                                                           >  2:710396/1‑104 (MQ=255)                                                                                                 aCTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGa                                 <  2:1114378/139‑1 (MQ=255)                                                                                                      tttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCa                                                                                            >  2:732163/1‑75 (MQ=255)                                                                                                      tttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCa                                                                                            <  1:732163/75‑1 (MQ=255)                                                                                                      tttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                            >  2:1290243/1‑139 (MQ=255)                                                                                                      tttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                            >  2:334418/1‑139 (MQ=255)                                                                                                       ttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                 <  1:263843/69‑1 (MQ=255)                                                                                                       ttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                 >  2:263843/1‑69 (MQ=255)                                                                                                        tACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATc                                                                                                                    >  1:34708/1‑49 (MQ=255)                                                                                                        tACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATc                                                                                                                    <  2:34708/49‑1 (MQ=255)                                                                                                         aCGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGc                         <  2:380200/139‑1 (MQ=255)                                                                                                           gggCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAg                       <  1:334418/139‑1 (MQ=255)                                                                                                               gTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATc                   >  2:862234/1‑139 (MQ=255)                                                                                                               gTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATc                   >  2:155759/1‑139 (MQ=255)                                                                                                                        tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgc          <  1:689236/139‑1 (MQ=255)                                                                                                                        tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgc          <  1:210082/139‑1 (MQ=255)                                                                                                                        tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgc          <  1:155759/139‑1 (MQ=255)                                                                                                                         cTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc         <  1:495981/139‑1 (MQ=255)                                                                                                                         cTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc         <  2:1205737/139‑1 (MQ=255)                                                                                                                          tACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                               >  2:276406/1‑52 (MQ=255)                                                                                                                          tACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                               <  1:276406/52‑1 (MQ=255)                                                                                                                               tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCt                                                                                                      >  1:482001/1‑40 (MQ=255)                                                                                                                               tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCt                                                                                                      <  2:482001/40‑1 (MQ=255)                                                                                                                               tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                               >  1:599107/1‑47 (MQ=255)                                                                                                                               tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                               <  2:599107/47‑1 (MQ=255)                                                                                                                                gCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGc                         <  1:155923/116‑1 (MQ=255)                                                                                                                                gCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGc                         >  2:155923/1‑116 (MQ=255)                                                                                                                                gCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt  >  2:934980/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                      CCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAT  >  NC_000913/4331502‑4331767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑