Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,419,456	T→C	N57S (AAC→AGC)	ulaG ←	L‑ascorbate 6‑phosphate lactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,419,456	0	T	C	100.0%	182.8 / NA	51	N57S (AAC→AGC)	ulaG	L‑ascorbate 6‑phosphate lactonase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

AACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTC  >  NC_000913/4419327‑4419584                                                                                                                                  |                                                                                                                                 aaCGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCAc                                                                                                                         >  1:1110962/1‑139 (MQ=255)       gtggtACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCg                                                                                                                   >  2:1044564/1‑139 (MQ=255)            aCGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTc                                                                                                              <  1:1044564/139‑1 (MQ=255)                            aGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGc                                                                                              <  2:1138688/139‑1 (MQ=255)                               tttttCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                           <  2:443331/139‑1 (MQ=255)                                 tttCACGCCAGCCATGCGCTGCATCTGGTGTCCCTGTTTGATTAACGGGTTACCGTGACGTTGTTTGCCAATGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagcct                                                                                         >  2:738773/1‑138 (MQ=255)                                        ccagccaTGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGccacc                                                                                  >  2:545923/1‑139 (MQ=255)                                                  gcTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa                                                                        <  2:176589/139‑1 (MQ=255)                                                      cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagcca                                                                                         <  2:176564/118‑1 (MQ=255)                                                      cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagcca                                                                                         >  1:176564/1‑118 (MQ=255)                                                      cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                           >  1:613260/1‑116 (MQ=255)                                                      cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                           <  2:613260/116‑1 (MQ=255)                                                        tCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGcc                                                                  >  2:731425/1‑139 (MQ=255)                                                                cccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTc                                                                                                                     <  1:411957/80‑1 (MQ=255)                                                                cccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTc                                                                                                                     >  2:411957/1‑80 (MQ=255)                                                                cccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTc                                                                                                              <  1:905912/87‑1 (MQ=255)                                                                cccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTc                                                                                                              >  2:905912/1‑87 (MQ=255)                                                                cccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGa                                                          >  2:775950/1‑139 (MQ=255)                                                                 ccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGAc                                                         >  2:349488/1‑139 (MQ=255)                                                                     tttCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAg                                                             >  2:698104/1‑131 (MQ=255)                                                                     tttCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAg                                                             <  1:698104/131‑1 (MQ=255)                                                                           tAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGt                                               >  1:1177530/1‑139 (MQ=255)                                                                             aCGGTTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTc                                             <  1:967689/139‑1 (MQ=255)                                                                               gggTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGcc                                                                                                                           <  2:586556/59‑1 (MQ=255)                                                                               gggTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGcc                                                                                                                           >  1:586556/1‑59 (MQ=255)                                                                                 gTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCa                                                                          >  2:1006488/1‑106 (MQ=255)                                                                                 gTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCa                                                                          <  1:1006488/106‑1 (MQ=255)                                                                                    aCCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTc                                                   <  1:374105/126‑1 (MQ=255)                                                                                    aCCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTc                                                   >  2:374105/1‑126 (MQ=255)                                                                                           cTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCttcattca                               <  2:1177530/139‑1 (MQ=255)                                                                                            tttgtttgCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCaa                              <  2:77978/139‑1 (MQ=255)                                                                                            tttgtttgCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCaa                              <  1:731425/139‑1 (MQ=255)                                                                                              tgtttgCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTTCGATTTCAACCAGATCCCGGTTCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTGCAATTTCTTCATTCAAcc                            <  1:738773/139‑1 (MQ=255)                                                                                                  tgCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTTTTCAATTTCTTCATTCAACCAGCt                        <  2:910903/139‑1 (MQ=255)                                                                                                   gCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGTAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTa                       >  2:619623/1‑139 (MQ=255)                                                                                                    ccAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGc                                                                   >  1:692663/1‑94 (MQ=255)                                                                                                    ccAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGc                                                                   <  2:692663/94‑1 (MQ=255)                                                                                                    ccAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAg                          <  2:1315005/135‑1 (MQ=255)                                                                                                    ccAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAg                          >  1:1315005/1‑135 (MQ=255)                                                                                                             cACCAGAAATCAACGCAAACGCTGGTGCCACCTTc                                                                                                                     <  1:798635/35‑1 (MQ=255)                                                                                                             cACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                           >  2:295161/1‑61 (MQ=255)                                                                                                             cACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                           <  1:295161/61‑1 (MQ=255)                                                                                                             cACCAGAAATCAACGCAAACGCTGGTGCCACCATc                                                                                                                     >  2:798635/1‑35 (MQ=25)                                                                                                                  gAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCg                                                           <  2:1292603/88‑1 (MQ=255)                                                                                                                  gAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCg                                                           >  1:1292603/1‑88 (MQ=255)                                                                                                                  gAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGaaa        >  2:1324520/1‑139 (MQ=255)                                                                                                                        aaCGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTc  >  2:1020585/1‑139 (MQ=255)                                                                                                                          cGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                           >  1:997997/1‑48 (MQ=255)                                                                                                                          cGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                           <  2:997997/48‑1 (MQ=255)                                                                                                                          cGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa                                                                        >  1:338670/1‑67 (MQ=255)                                                                                                                          cGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa                                                                        <  2:338670/67‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 AACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTC  >  NC_000913/4419327‑4419584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑