Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,424,516 C→T *92* (TAG→TAA yjfY ← YhcN family protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,5160CT100.0% 103.7 / NA 33*92* (TAG→TAAyjfYYhcN family protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/17);  total (16/17)

CGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGA  >  NC_000913/4424391‑4424652
                                                                                                                             |                                                                                                                                        
cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg                                                                                                                                       <  2:239073/129‑1 (MQ=255)
cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg                                                                                                                                       >  1:239073/1‑129 (MQ=255)
cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAatat                                                                                                                             >  1:500103/1‑139 (MQ=255)
 gcgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGAt                                                                                                                                   <  2:235453/132‑1 (MQ=255)
 gcgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGAt                                                                                                                                   >  1:235453/1‑132 (MQ=255)
   gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAatat                                                                                                                             <  2:253917/136‑1 (MQ=255)
   gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAatat                                                                                                                             >  1:253917/1‑136 (MQ=255)
          aaaTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGc                                                                                                                   <  2:125997/139‑1 (MQ=255)
                                            ccGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCa                                                                                <  2:363748/139‑1 (MQ=255)
                                               cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg                                                                                                                                       >  1:1262053/1‑82 (MQ=255)
                                               cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAg                                                                                                                                       <  2:1262053/82‑1 (MQ=255)
                                               cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCa                                                                                   >  2:916338/1‑133 (MQ=255)
                                               cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCCGTTCCCGCa                                                                                   <  1:916338/133‑1 (MQ=255)
                                                     tttttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCg                                                                                                      >  1:902812/1‑108 (MQ=255)
                                                     tttttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCg                                                                                                      <  2:902812/108‑1 (MQ=255)
                                                                                   gcaaagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGttt                                         <  2:500103/139‑1 (MQ=255)
                                                                                            aCTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                        >  1:651439/1‑99 (MQ=255)
                                                                                            aCTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                        <  2:651439/99‑1 (MQ=255)
                                                                                               aTGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                          <  2:284972/94‑1 (MQ=255)
                                                                                               aTGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                          >  1:284972/1‑94 (MQ=255)
                                                                                                 ggCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGcc                           >  2:418897/1‑139 (MQ=255)
                                                                                                   cAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTgatat                                                                                                            >  2:1177299/1‑53 (MQ=255)
                                                                                                   cAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTgatat                                                                                                            <  1:1177299/57‑5 (MQ=255)
                                                                                                      tGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCgcgc                                                                                                                       <  2:1166233/43‑1 (MQ=255)
                                                                                                      tGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCgcgc                                                                                                                       >  1:1166233/1‑43 (MQ=255)
                                                                                                      tGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCg                                                                                                                  >  2:413998/1‑48 (MQ=255)
                                                                                                      tGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCg                                                                                                                  <  1:413998/48‑1 (MQ=255)
                                                                                                           cGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                          >  2:168935/1‑82 (MQ=255)
                                                                                                           cGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                          <  1:168935/82‑1 (MQ=255)
                                                                                                                    taCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                          >  1:1111218/1‑73 (MQ=255)
                                                                                                                    taCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                          <  2:1111218/73‑1 (MQ=255)
                                                                                                                    taCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                          >  1:70048/1‑73 (MQ=255)
                                                                                                                    taCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                          <  2:70048/73‑1 (MQ=255)
                                                                                                                          gtgtTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                        <  1:656896/69‑1 (MQ=255)
                                                                                                                          gtgtTAGCGATAAATATCCGCGCTGGCGTGATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa  >  2:378531/1‑139 (MQ=255)
                                                                                                                             |                                                                                                                                        
CGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGA  >  NC_000913/4424391‑4424652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: