Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,441,538	C→T	*213* (TAG→TAA)	msrA ←	methionine sulfoxide reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,441,538	0	C	T	100.0%	186.2 / NA	58	*213* (TAG→TAA)	msrA	methionine sulfoxide reductase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/26);  total (32/26)

TATGCCGATCAGGCCTACGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGCTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTG  >  NC_000913/4441404‑4441672                                                                                                                                       |                                                                                                                                       tATGCCGATCAGGCCTACGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATg                                                                                                                                    >  1:628520/1‑139 (MQ=255)                       tCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgcc                                                                                                              >  2:1288823/1‑139 (MQ=255)                       tCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgcc                                                                                                              >  1:274292/1‑139 (MQ=255)                            gCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTGACGTTATGCTTCCGGCGGCAGACAGAcgccaattc                                                                                                         >  1:813774/1‑139 (MQ=255)                             cAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattcc                                                                                                        <  1:12715/139‑1 (MQ=255)                                     tGAATTTGGTCGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgccaattc                                                                                                <  2:795293/139‑1 (MQ=255)                                     tGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgccaattc                                                                                                >  2:411606/1‑139 (MQ=255)                                       aaTTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGTTTCCGGCGGCAGACAGAcgccaattccgcc                                                                                                     >  2:703433/1‑132 (MQ=255)                                       aaTTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGTTTCCGGCGGCAGACAGAcgccaattccgcc                                                                                                     <  1:703433/132‑1 (MQ=255)                                           tGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGt                                                                                          <  2:1198284/139‑1 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCcgg                                                                                                                             >  2:397866/1‑103 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCcgg                                                                                                                             <  1:397866/103‑1 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGt                                                                                          <  1:1205346/138‑1 (MQ=255)                                            ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGt                                                                                          >  2:1205346/1‑138 (MQ=255)                                                   tttGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcaga                                                                                                                      >  1:819133/1‑103 (MQ=255)                                                   tttGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcaga                                                                                                                      <  2:819133/103‑1 (MQ=255)                                                       tAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGt                                                                              >  2:1217455/1‑139 (MQ=255)                                                            cTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcaga                                                                                                                      >  2:322547/1‑94 (MQ=255)                                                            cTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcaga                                                                                                                      <  1:322547/94‑1 (MQ=255)                                                            cTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTa                                                                         >  2:1195128/1‑139 (MQ=255)                                                             tGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                        <  2:813774/139‑1 (MQ=255)                                                                 aaGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAg                                                                    <  2:628520/139‑1 (MQ=255)                                                                    tCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGata                                                                 >  2:812039/1‑139 (MQ=255)                                                                              cgcATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcaga                                                                                                                      <  2:1238813/76‑1 (MQ=255)                                                                              cgcATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcaga                                                                                                                      >  1:1238813/1‑76 (MQ=255)                                                                                     ggCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCtt                                                <  1:1195128/139‑1 (MQ=255)                                                                                      gCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTc                                               >  2:909677/1‑139 (MQ=255)                                                                                        aTAGACAAAGAGCACTTTATCAACAAACTACATTTATACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCgg                                             <  1:812039/139‑1 (MQ=255)                                                                                          aGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCa                                           >  2:76200/1‑139 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                        >  2:677530/1‑104 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                        <  1:677530/104‑1 (MQ=255)                                                                                                agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCAtaataa                                     >  2:279225/1‑139 (MQ=255)                                                                                                      cTTTATCAACAAACTACATTTGTACCCGTAACGTTCTGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGgt                               >  2:997788/1‑139 (MQ=255)                                                                                                         tATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACgg                                <  2:245250/135‑1 (MQ=255)                                                                                                         tATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACgg                                >  1:245250/1‑135 (MQ=255)                                                                                                         tATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGtgtg                            >  2:369463/1‑139 (MQ=255)                                                                                                                 aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCca                                                                                              >  1:242255/1‑65 (MQ=255)                                                                                                                 aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCca                                                                                              <  2:242255/65‑1 (MQ=255)                                                                                                                 aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACggg                                                                               >  1:941865/1‑80 (MQ=255)                                                                                                                 aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACggg                                                                               <  2:941865/80‑1 (MQ=255)                                                                                                                 aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGgtg                              <  2:414357/129‑1 (MQ=255)                                                                                                                 aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGgtg                              >  1:414357/1‑129 (MQ=255)                                                                                                                   cTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGa                  >  1:934580/1‑139 (MQ=255)                                                                                                                    tACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgcc                                                                                                     <  2:1074144/55‑1 (MQ=255)                                                                                                                    tACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgcc                                                                                                     >  1:1074144/1‑55 (MQ=255)                                                                                                                    tACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGAt                 <  1:997788/139‑1 (MQ=255)                                                                                                                    tACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGAt                 <  1:909677/139‑1 (MQ=255)                                                                                                                       aTTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAAcc                                                                                      <  1:1019745/67‑1 (MQ=255)                                                                                                                       aTTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAAcc                                                                                      >  2:1019745/1‑67 (MQ=255)                                                                                                                         ttGTACCCGTAACGTTATGCTTCCGGCGGCAGGCAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCg            >  2:800001/1‑139 (MQ=255)                                                                                                                              cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCcaca                                                                                            <  2:1031342/54‑1 (MQ=255)                                                                                                                              cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCcaca                                                                                            >  1:1031342/1‑54 (MQ=255)                                                                                                                              cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCg            >  2:277928/1‑134 (MQ=255)                                                                                                                              cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCg            <  1:277928/134‑1 (MQ=255)                                                                                                                                 gTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGAtg    <  1:1288823/139‑1 (MQ=255)                                                                                                                                   aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGc                    <  2:56239/121‑1 (MQ=255)                                                                                                                                   aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGc                    >  1:56239/1‑121 (MQ=255)                                                                                                                                   aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCTGTGGTGAtgtg  >  1:365360/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       TATGCCGATCAGGCCTACGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGCTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTG  >  NC_000913/4441404‑4441672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑