Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,464,629 G→A S27S (AGC→AGT)  treC ← trehalose‑6‑P hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,464,6290GA100.0% 173.7 / NA 54S27S (AGC→AGT) treCtrehalose‑6‑P hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/27);  total (27/27)

ATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAA  >  NC_000913/4464500‑4464762
                                                                                                                                 |                                                                                                                                     
ataGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCgtg                                                                                                                              <  2:486114/139‑1 (MQ=255)
    ttCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGgtgt                                                                                                                          >  2:944398/1‑139 (MQ=255)
    ttCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGgtgt                                                                                                                          <  1:944398/139‑1 (MQ=255)
              cgtAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGaaa                                                                                                                   <  2:1212262/136‑1 (MQ=255)
              cgtAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGaaa                                                                                                                   >  1:1212262/1‑136 (MQ=255)
                tAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCgtg                                                                                                                              <  1:483873/123‑1 (MQ=255)
                tAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCgtg                                                                                                                              >  2:483873/1‑123 (MQ=255)
                          cGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAAt                                                                                                    <  1:1299911/139‑1 (MQ=255)
                                 gggAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATaa                                                                                                      <  2:464968/130‑1 (MQ=255)
                                 gggAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATaa                                                                                                      >  1:464968/1‑130 (MQ=255)
                                           tAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGAt                                                                                                        <  1:1217018/118‑1 (MQ=255)
                                           tAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGAt                                                                                                        >  2:1217018/1‑118 (MQ=255)
                                           tAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGt                                                                                   <  2:336124/139‑1 (MQ=255)
                                           tAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGt                                                                                   <  2:780797/139‑1 (MQ=255)
                                                    gTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCNTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc                                                                          >  1:1125225/1‑139 (MQ=255)
                                                                  aTCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCTCGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAActct                                                                                                              <  1:900173/89‑1 (MQ=255)
                                                                  aTCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTAcccatg                                                                                                                              >  1:1138020/1‑70 (MQ=255)
                                                                  aTCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCatg                                                                                                                              <  2:1138020/73‑1 (MQ=255)
                                                                  aTCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAActct                                                                                                              >  2:900173/1‑89 (MQ=255)
                                                                  aTCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGcc                                                                             >  2:227097/1‑122 (MQ=255)
                                                                  aTCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGcc                                                                             <  1:227097/122‑1 (MQ=255)
                                                                                   gCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGcc                                                                             <  2:193251/105‑1 (MQ=255)
                                                                                   gCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGcc                                                                             >  1:193251/1‑105 (MQ=255)
                                                                                   gCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCAt                                                       >  2:2544/1‑127 (MQ=255)
                                                                                   gCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCAt                                                       <  1:2544/127‑1 (MQ=255)
                                                                                       aTAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATaa                                                                                                      >  1:1203023/1‑76 (MQ=255)
                                                                                       aTAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATaa                                                                                                      <  2:1203023/76‑1 (MQ=255)
                                                                                       aTAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc                                                                          <  1:628403/104‑1 (MQ=255)
                                                                                       aTAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc                                                                          >  2:628403/1‑104 (MQ=255)
                                                                                        tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCtt                                                                                                             <  2:404832/68‑1 (MQ=255)
                                                                                        tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCtt                                                                                                             >  1:404832/1‑68 (MQ=255)
                                                                                        tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc                                                                          >  2:300159/1‑103 (MQ=255)
                                                                                        tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc                                                                          <  1:300159/103‑1 (MQ=255)
                                                                                        tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTg                                                                      <  2:566178/107‑1 (MQ=255)
                                                                                        tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTg                                                                      >  1:566178/1‑107 (MQ=255)
                                                                                        tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGAt                                                              >  1:1118863/1‑115 (MQ=255)
                                                                                        tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGAt                                                              <  2:1118863/115‑1 (MQ=255)
                                                                                                gtgtTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAggg                              >  1:594258/1‑139 (MQ=255)
                                                                                                   ttGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTTGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAg                                                           >  1:526036/1‑107 (MQ=255)
                                                                                                   ttGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTTGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAg                                                           <  2:526036/107‑1 (MQ=255)
                                                                                                     ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGAt                                                                                                        <  1:864956/60‑1 (MQ=255)
                                                                                                     ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGAt                                                                                                        <  2:481249/60‑1 (MQ=255)
                                                                                                     ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGAt                                                                                                        >  1:481249/1‑60 (MQ=255)
                                                                                                     ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGAt                                                                                                        >  2:864956/1‑60 (MQ=255)
                                                                                                     ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGa                                                                  >  2:121646/1‑98 (MQ=255)
                                                                                                     ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGa                                                                  <  1:121646/98‑1 (MQ=255)
                                                                                                          aCGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTg                    <  1:1102473/139‑1 (MQ=255)
                                                                                                                          cGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACg                                                   >  2:1123484/1‑92 (MQ=255)
                                                                                                                          cGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACg                                                   <  1:1123484/92‑1 (MQ=255)
                                                                                                                           ggTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTgggg                                                                   >  1:992396/1‑75 (MQ=255)
                                                                                                                           ggTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTgggg                                                                   <  2:992396/75‑1 (MQ=255)
                                                                                                                           ggTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTa   >  1:288026/1‑139 (MQ=255)
                                                                                                                           ggTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTa   >  2:405367/1‑139 (MQ=255)
                                                                                                                            gTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTaa  >  2:672579/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                     
ATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAA  >  NC_000913/4464500‑4464762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑