Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,490,141 C→T *333* (TAG→TAA idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,490,1410CT100.0% 223.0 / NA 69*333* (TAG→TAAidnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/35);  total (34/35)

AAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCG  >  NC_000913/4490015‑4490276
                                                                                                                              |                                                                                                                                       
tcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                             >  1:1327093‑M2/113‑139 (MQ=255)
 cccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                            <  2:198989‑M2/28‑1 (MQ=255)
        gtaaaaacagttctgtgtccggcgtgcgggcaattaacaggagttcactcatgagatatcctccagtttcctgcttggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATa                                                                                                                     <  2:603970‑M2/35‑1 (MQ=255)
                agttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGAt                                                                                                             >  2:235932‑M2/97‑139 (MQ=255)
                   tctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAcc                                                                                                          >  1:664674‑M2/94‑139 (MQ=255)
                   tctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAcc                                                                                                          >  2:355681‑M2/94‑139 (MQ=255)
                    ctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                                         <  2:664674‑M2/47‑1 (MQ=255)
                            ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                 <  1:355681‑M2/55‑1 (MQ=255)
                            ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                 <  1:235932‑M2/55‑1 (MQ=255)
                               gtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                              <  1:1075894‑M2/58‑1 (MQ=255)
                                    ggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttg                                                                                         <  2:1327093‑M2/63‑1 (MQ=255)
                                    ggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          <  1:956859‑M2/62‑1 (MQ=255)
                                    ggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          >  2:956859‑M2/77‑138 (MQ=255)
                                                 ggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGt                                                                            >  2:182124‑M2/64‑139 (MQ=255)
                                                  gttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGtt                                                                           <  1:182124‑M2/77‑1 (MQ=255)
                                                   ttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa                                                                                                                                     <  2:1120655‑M2/19‑1 (MQ=255)
                                                   ttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa                                                                                                                                     >  1:1120655‑M2/62‑80 (MQ=255)
                                                   ttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTa                                                                          >  2:613527‑M2/62‑139 (MQ=255)
                                                               atatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                      <  2:22472‑M2/50‑1 (MQ=255)
                                                               atatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                      >  1:22472‑M2/50‑99 (MQ=255)
                                                               atatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTg                                                              >  1:146053‑M2/50‑139 (MQ=255)
                                                                 atcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          <  2:492063‑M2/62‑1 (MQ=255)
                                                                 atcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          >  1:492063‑M2/48‑109 (MQ=255)
                                                                  tcctccagtttgctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa                                                                                                                                     <  2:222606‑M2/19‑1 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa                                                                                                                                     >  1:222606‑M2/47‑65 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                 <  1:664099‑M2/55‑1 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                 >  2:664099‑M2/47‑101 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAAc                                                                                                <  1:1164603‑M2/56‑1 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAAc                                                                                                >  2:1164603‑M2/47‑102 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTc                                                           >  1:1126808‑M2/47‑139 (MQ=255)
                                                                    ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                                         >  1:1300127‑M2/45‑91 (MQ=255)
                                                                    ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                                         <  2:1300127‑M2/47‑1 (MQ=255)
                                                                            tcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          >  2:992101‑M2/37‑98 (MQ=255)
                                                                            tcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          <  1:992101‑M2/62‑1 (MQ=255)
                                                                             cctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAATTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTg                                                >  2:949520‑M2/36‑139 (MQ=255)
                                                                               tgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTggg                                              >  2:1064753‑M2/34‑139 (MQ=255)
                                                                                 cctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGggcg                                            <  1:1064753‑M2/108‑1 (MQ=255)
                                                                                    ggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGTGTGTTGCCGTGATAAATCTGATAccc                                                                                                         <  2:1294694‑M2/47‑1 (MQ=255)
                                                                                    ggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGTGTGTTGCCGTGATAAATCTGATAccc                                                                                                         >  1:1294694‑M2/29‑75 (MQ=255)
                                                                                       gtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc                                      <  1:613527‑M2/114‑1 (MQ=255)
                                                                                          taggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttgt                                                                                        >  1:144094‑M2/23‑86 (MQ=255)
                                                                                          taggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttgt                                                                                        <  2:144094‑M2/64‑1 (MQ=255)
                                                                                           aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGa                                                                                                                       <  1:804065‑M2/33‑1 (MQ=255)
                                                                                           aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGa                                                                                                                       >  2:804065‑M2/22‑54 (MQ=255)
                                                                                           aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAgtg                                                                                            >  1:379939‑M2/22‑81 (MQ=255)
                                                                                           aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAgtg                                                                                            <  2:379939‑M2/60‑1 (MQ=255)
                                                                                           aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCg                                                                             >  1:430537‑M2/22‑96 (MQ=255)
                                                                                           aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCg                                                                             <  2:430537‑M2/75‑1 (MQ=255)
                                                                                            ggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                            >  2:321748‑M2/21‑48 (MQ=255)
                                                                                            ggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                            <  1:321748‑M2/28‑1 (MQ=255)
                                                                                            ggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGAcc                                 <  1:949520‑M2/119‑1 (MQ=255)
                                                                                               aacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAat                              >  2:655317‑M2/18‑139 (MQ=255)
                                                                                                acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGAcc                                 <  1:354554‑M2/119‑1 (MQ=255)
                                                                                                acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGAcc                                 >  2:354554‑M2/17‑135 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          <  2:239228‑M2/62‑1 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          >  1:239228‑M2/16‑77 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGc                                                             >  2:1102446‑M2/16‑106 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGc                                                             <  1:1102446‑M2/91‑1 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTg                                                <  2:908256‑M2/104‑1 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAatat                            >  1:254095‑M2/16‑139 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAACCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTg                                                >  1:908256‑M2/16‑119 (MQ=255)
                                                                                                  gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                              <  1:1343257‑M2/58‑1 (MQ=255)
                                                                                                  gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                              >  2:1343257‑M2/15‑72 (MQ=255)
                                                                                                        caaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACg                     <  2:1126808‑M2/131‑1 (MQ=255)
                                                                                                                 aaGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAAt            >  2:838148/1‑139 (MQ=255)
                                                                                                                    agaAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTAc         <  1:838148/139‑1 (MQ=255)
                                                                                                                         aTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTgg    >  2:11432/1‑139 (MQ=255)
                                                                                                                          tAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGc   <  2:146053/139‑1 (MQ=255)
                                                                                                                           aaCTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCg  <  2:254095/139‑1 (MQ=255)
                                                                                                                              |                                                                                                                                       
AAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCG  >  NC_000913/4490015‑4490276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: