Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,515,284	C→T	G465D (GGC→GAC)	fecA ←	TonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,515,284	0	C	T	100.0%	132.0 / NA	42	G465D (GGC→GAC)	fecA	TonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/20);  total (22/20)

TTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCA  >  NC_000913/4515149‑4515418                                                                                                                                        |                                                                                                                                       ttgttCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCgg                                                                                                                                     >  1:588572/1‑139 (MQ=255)         gTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAAc                                                                                                                             >  2:724017/1‑139 (MQ=255)             gACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGcc                                                                                                                         <  1:106917/139‑1 (MQ=255)              aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc                                                                                                                        >  1:655415/1‑139 (MQ=255)              aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc                                                                                                                        >  1:493752/1‑139 (MQ=255)                         tCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCTGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                             <  1:724017/139‑1 (MQ=255)                         tCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                             <  2:757553/139‑1 (MQ=255)                         tCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                             <  2:107129/139‑1 (MQ=255)                          cGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGc                                                                                                            <  2:655415/139‑1 (MQ=255)                              aCGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGgtg                                                                                                        >  2:1009940/1‑139 (MQ=255)                                    aCCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTaa                                                                                                  <  1:720869/139‑1 (MQ=255)                                           gTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCAttt                                                                                           <  2:493752/139‑1 (MQ=255)                                                 gTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCg                                                                                     <  1:1009940/139‑1 (MQ=255)                                                       ttGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGCCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCAATTCATGCGTTGatt                                                                               <  1:817832/139‑1 (MQ=255)                                                            gATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGattcattc                                                                          >  2:280499/1‑139 (MQ=255)                                                                        gTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAg                                                              <  1:280499/139‑1 (MQ=255)                                                                                 aTACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                                          <  1:1293549/86‑1 (MQ=255)                                                                                 aTACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                                          >  2:1293549/1‑86 (MQ=255)                                                                                 aTACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcccacgccc                                                     >  2:217616/1‑139 (MQ=255)                                                                                       ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCg                                               >  2:997081/1‑139 (MQ=255)                                                                                       ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCg                                               >  1:6151/1‑139 (MQ=255)                                                                                        gCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                             >  1:224128/1‑76 (MQ=255)                                                                                        gCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                             <  2:224128/76‑1 (MQ=255)                                                                                          gTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgc                                            >  1:587319/1‑139 (MQ=255)                                                                                           tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCa                                                                                                                               >  2:1133945/1‑55 (MQ=255)                                                                                           tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCa                                                                                                                               <  1:1133945/55‑1 (MQ=255)                                                                                             cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGgtg                                                                                                        >  2:1132253/1‑76 (MQ=255)                                                                                             cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGgtg                                                                                                        <  1:1132253/76‑1 (MQ=255)                                                                                             cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGcgcg                                         >  2:340901/1‑139 (MQ=255)                                                                                                               cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcccacg                                                        <  1:890908/106‑1 (MQ=255)                                                                                                               cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcccacg                                                        >  2:890908/1‑106 (MQ=255)                                                                                                               cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAg                       >  1:407847/1‑139 (MQ=255)                                                                                                                 tATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGCTTCATTCAAAt                                                                      >  2:823651/1‑90 (MQ=255)                                                                                                                 tATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCTTTCAAAt                                                                      <  1:823651/90‑1 (MQ=255)                                                                                                                        gTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTg              >  2:25247/1‑139 (MQ=255)                                                                                                                         tCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGt             >  2:242869/1‑139 (MQ=255)                                                                                                                             aaGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg         <  1:340901/139‑1 (MQ=255)                                                                                                                             aaGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg         <  1:217616/139‑1 (MQ=255)                                                                                                                                   ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTc   >  2:935653/1‑139 (MQ=255)                                                                                                                                    tGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg         <  2:553913/132‑1 (MQ=255)                                                                                                                                    tGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg         >  1:553913/1‑132 (MQ=255)                                                                                                                                    tGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCa  <  2:587319/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCA  >  NC_000913/4515149‑4515418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑