Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,464	C→A	L129F (TTG→TTT)	yjiM ←	putative 2‑hydroxyglutaryl‑CoA dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,464	0	C	A	100.0%	136.6 / NA	44	L129F (TTG→TTT)	yjiM	putative 2‑hydroxyglutaryl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/21);  total (23/21)

TTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGC  >  NC_000913/4565332‑4565588                                                                                                                                     |                                                                                                                               tttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGc                                                                                                                          <  2:244959/139‑1 (MQ=255)            tGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAAc                                                                                                             >  1:725292/1‑139 (MQ=255)                            aTCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGcc                                                                                             <  1:1191914/139‑1 (MQ=255)                               ttCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATg                                                                                          <  2:725292/139‑1 (MQ=255)                                 cGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTa                                                                                        >  2:594307/1‑139 (MQ=255)                                   cTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCa                                                                                            >  2:627669/1‑133 (MQ=255)                                   cTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCa                                                                                            <  1:627669/133‑1 (MQ=255)                                           gTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCAt                                                                                           >  2:966673/1‑126 (MQ=255)                                           gTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCAt                                                                                           <  1:966673/126‑1 (MQ=255)                                           gTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACAtt                                                                              >  1:1004403/1‑139 (MQ=255)                                           gTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACAtt                                                                              >  2:175910/1‑139 (MQ=255)                                            tGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACAttt                                                                             >  1:862109/1‑139 (MQ=255)                                              cccAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTaaa                                                                                                    >  2:904236/1‑114 (MQ=255)                                              cccAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTaaa                                                                                                    <  1:904236/114‑1 (MQ=255)                                                cAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa                                                                                                              >  1:415005/1‑102 (MQ=255)                                                cAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa                                                                                                              <  2:415005/102‑1 (MQ=255)                                                        tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa                                                                                                              >  2:204204/1‑94 (MQ=255)                                                        tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa                                                                                                              <  1:204204/94‑1 (MQ=255)                                                        tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATg                                                                                          <  1:344175/114‑1 (MQ=255)                                                        tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATg                                                                                          >  2:344175/1‑114 (MQ=255)                                                        tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGt                                                                 <  1:381840/139‑1 (MQ=255)                                                             tACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAg                                                            >  2:517409/1‑139 (MQ=255)                                                              aCCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAgg                                                           <  1:517409/139‑1 (MQ=255)                                                               ccGTATTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTCTTCATACATTTTCTTTTTGCCGTCGCAggt                                                          >  2:693495/1‑139 (MQ=255)                                                                  ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa                                                                                                              <  1:1094412/84‑1 (MQ=255)                                                                  ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa                                                                                                              >  2:1094412/1‑84 (MQ=255)                                                                  ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACt                                                                                                  <  1:208551/96‑1 (MQ=255)                                                                  ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACt                                                                                                  >  2:208551/1‑96 (MQ=255)                                                                      ttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCa                                                   <  1:175910/139‑1 (MQ=255)                                                                                       cGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCa                                                             <  1:135299/112‑1 (MQ=255)                                                                                       cGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCa                                                             >  2:135299/1‑112 (MQ=255)                                                                                          cTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGaaaa                               <  2:1004403/139‑1 (MQ=255)                                                                                               cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACt                                                                                                  >  1:401923/1‑67 (MQ=255)                                                                                               cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACt                                                                                                  <  2:401923/67‑1 (MQ=255)                                                                                               cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGt                                                                 >  2:181257/1‑100 (MQ=255)                                                                                               cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGt                                                                 <  1:181257/100‑1 (MQ=255)                                                                                                    cgcacgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAaagtagaagtag                     <  1:594307/139‑1 (MQ=255)                                                                                                        cgcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGc                 >  2:415562/1‑139 (MQ=255)                                                                                                         gcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCa                >  2:148492/1‑139 (MQ=255)                                                                                                             ggCATCGTCCTTAACGCTGTTGGGAAATTGCATCACACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGaa                                 >  2:563658/1‑120 (MQ=255)                                                                                                             ggCATCGTCCTTAACGCTGTTGGGAAATTGCATCACACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGaa                                 <  1:563658/120‑1 (MQ=255)                                                                                                                       ttAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGa                                               <  1:1008739/94‑1 (MQ=255)                                                                                                                       ttAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGc  >  2:1333317/1‑139 (MQ=255)                                                                                                                       ttAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCCTAAACTCCGCCATATATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGa                                               >  2:1008739/1‑94 (MQ=255)                                                                                                                                     |                                                                                                                               TTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGC  >  NC_000913/4565332‑4565588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑