Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,595,527	T→C	T109A (ACG→GCG)	lgoR ←	putative transcriptional activator for L‑galactonate catabolism

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,595,527	0	T	C	100.0%	192.6 / NA	53	T109A (ACG→GCG)	lgoR	putative transcriptional activator for L‑galactonate catabolism
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/24);  total (29/24)

CGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGT  >  NC_000913/4595419‑4595661                                                                                                             |                                                                                                                                       cgcgCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgcgc                                                                                                                  >  2:822123/1‑131 (MQ=255) cgcgCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgcgc                                                                                                                  <  1:822123/131‑1 (MQ=255)                           taACGGCCGAATTTTAAACGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTNCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAaca                                                                                >  2:465053/1‑139 (MQ=255)                           taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGGCTGCTCa                                                                                    >  1:881137/1‑135 (MQ=255)                           taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCa                                                                                    <  2:881137/135‑1 (MQ=255)                           taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAaca                                                                                >  2:396923/1‑139 (MQ=255)                                 ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc                                                                                                                    <  2:768619/97‑1 (MQ=255)                                 ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc                                                                                                                    >  1:768619/1‑97 (MQ=255)                                 ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgt                                                                          <  2:472472/139‑1 (MQ=255)                                 ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgt                                                                          <  2:899601/139‑1 (MQ=255)                                       tttAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTttgttttgtt                                                                    <  1:396923/139‑1 (MQ=255)                                                              aaCCGGGCTGACACCCGCGGCCCGAACCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAg                                             >  1:787626/1‑139 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAAc                                                                                                                        >  1:852597/1‑63 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAAc                                                                                                                        <  2:852597/63‑1 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc                                                                                                                    <  2:294698/67‑1 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc                                                                                                                    >  1:294698/1‑67 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                          >  1:987055/1‑93 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                          <  2:987055/93‑1 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   >  1:384184/1‑100 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   <  2:384184/100‑1 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACt                                                                              >  2:110578/1‑105 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACt                                                                              <  1:110578/105‑1 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                            >  1:770532/1‑139 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                            >  2:1097999/1‑139 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                            >  1:964738/1‑139 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                            >  1:178532/1‑139 (MQ=255)                                                               aCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAgg                                            >  1:357782/1‑139 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGgc                                                                                                                    >  1:294517/1‑63 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   >  1:1304245/1‑96 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   >  1:1335746/1‑96 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   <  2:1304245/96‑1 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                   <  2:1335746/96‑1 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAg                                                        >  2:973407/1‑123 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAg                                                        <  1:973407/123‑1 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaa                                        >  1:1001906/1‑139 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaa                                        >  2:913271/1‑139 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaa                                        >  1:856097/1‑139 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaa                                        >  2:750194/1‑139 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTAGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaa                                        >  1:846481/1‑139 (MQ=255)                                                                   ggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCGGGGCGTAACTGgc                                                                                                                    <  2:294517/63‑1 (MQ=255)                                                                            ccGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtcatc                               <  1:1097999/139‑1 (MQ=255)                                                                              gcgGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGc                                                       >  2:59821/1‑113 (MQ=255)                                                                              gcgGCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGc                                                       <  1:59821/113‑1 (MQ=255)                                                                                 gCCCGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGt                          <  2:964738/139‑1 (MQ=255)                                                                                  cccGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTc                                                                                     <  2:886160/79‑1 (MQ=255)                                                                                  cccGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttg                                                                           <  1:347710/89‑1 (MQ=255)                                                                                  cccGAGCCAGTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttg                                                                           >  2:347710/1‑89 (MQ=255)                                                                                  cccGAGCCAGTTGCAGTTAAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTc                                                                                     >  1:886160/1‑79 (MQ=255)                                                                                           gTTGCAGTTCAGAAAATGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                <  1:913271/139‑1 (MQ=255)                                                                                           gTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGTTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                <  2:846481/139‑1 (MQ=255)                                                                                           gTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                <  1:750194/139‑1 (MQ=255)                                                                                           gTTGCAGTTCAGAAAACGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAcg                <  2:856097/139‑1 (MQ=255)                                                                                                         aaCGCTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGt  >  1:654112/1‑139 (MQ=255)                                                                                                             |                                                                                                                                       CGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGT  >  NC_000913/4595419‑4595661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑