Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,604,202	G→A	*242* (TAG→TAA)  E15K (GAA→AAA)	yjjQ → bglJ →	putative transcriptional regulator bgl operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,202	0	G	A	97.4%	240.2 / ‑0.7	76	coding (726/726 nt) coding (43/678 nt)	yjjQ bglJ	putative transcriptional regulator bgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (39/35);  minor base . (1/1);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

CTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC  >  NC_000913/4604081‑4604332                                                                                                                          |                                                                                                                                   cTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGagt                                                                                                                   <  1:1110171/139‑1 (MQ=255)           tGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta                                                                                                               <  2:1064014/133‑1 (MQ=255)           tGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta                                                                                                               >  1:1064014/1‑133 (MQ=255)             cATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTAtt                                                                                                       <  1:1286010/139‑1 (MQ=255)              aTGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         <  1:916332/120‑1 (MQ=255)              aTGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTc                                                                                                                         >  2:916332/1‑120 (MQ=255)                ggATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTgag                                                                                                    >  1:999733/1‑139 (MQ=255)                          cgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGt                                                                                                                          <  2:1052806/107‑1 (MQ=255)                          cgcgcAGGGTGCAAGAGAACTTAACTTGCCATCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGt                                                                                                                          >  1:1052806/1‑107 (MQ=255)                           gcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAg                                                                                         >  1:1056925/1‑139 (MQ=255)                            cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta                                                                                                               >  2:245948/1‑116 (MQ=255)                            cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta                                                                                                               <  1:245948/116‑1 (MQ=255)                            cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGa                                                                                        >  2:677434/1‑139 (MQ=255)                            cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGa                                                                                        >  1:966825/1‑139 (MQ=255)                            cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGa                                                                                        >  2:835660/1‑139 (MQ=255)                               aGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta                                                                                                               <  1:251908/113‑1 (MQ=255)                               aGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAgtagta                                                                                                               >  2:251908/1‑113 (MQ=255)                                 ggTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGtt                                                                                   >  2:859178/1‑139 (MQ=255)                                 ggTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGtt                                                                                   >  2:915633/1‑139 (MQ=255)                                      aaGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGTATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCgg                                                                              >  1:105637/1‑139 (MQ=255)                                       atcgaACTTAACTTGCCTTCTGTTTCTGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCggg                                                                             <  2:294801/136‑1 (MQ=255)                                       agagAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCggg                                                                             <  2:1056925/139‑1 (MQ=255)                                        gagaACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGTATGGAACACAGCCGAATAAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGt                                                                            <  2:105637/139‑1 (MQ=255)                                          gaACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTaa                                                                          <  1:915633/139‑1 (MQ=255)                                           aacttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTg                                                                                                      <  2:28954/110‑1 (MQ=255)                                           aacttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTg                                                                                                      >  1:28954/1‑110 (MQ=255)                                             cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAccc                                                                       >  2:497379/1‑139 (MQ=255)                                             cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAccc                                                                       >  1:971850/1‑139 (MQ=255)                                             cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAccc                                                                       >  2:629469/1‑139 (MQ=255)                                             cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAAccc                                                                       >  2:92647/1‑139 (MQ=255)                                                       cTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             <  2:999733/139‑1 (MQ=255)                                                        ttCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc                                                            <  2:971850/139‑1 (MQ=255)                                                        ttCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc                                                            <  2:966825/139‑1 (MQ=255)                                                             tttATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATAcc                                                       >  1:404225/1‑139 (MQ=255)                                                                aTGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCAT‑‑AAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTtat                                                                   >  1:1338032/1‑122 (MQ=255)                                                                aTGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCAT‑‑AAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTtat                                                                   <  2:1338032/122‑1 (MQ=255)                                                                     gaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAATTGCGTCATGag                                                                                                                    <  2:576223/70‑1 (MQ=255)                                                                     gaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAATTGCGTCATGag                                                                                                                    >  1:576223/1‑70 (MQ=255)                                                                     gaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag                                                                                                                    >  2:321996/1‑70 (MQ=255)                                                                     gaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGag                                                                                                                    <  1:321996/70‑1 (MQ=255)                                                                     gaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGt                                                                            <  2:439171/110‑1 (MQ=255)                                                                     gaTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGt                                                                            >  1:439171/1‑110 (MQ=255)                                                                      aTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGt                                              <  1:835660/139‑1 (MQ=255)                                                                      aTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGt                                              <  1:677434/139‑1 (MQ=255)                                                                       tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAaaaa                                                                                                                                 <  1:60833/55‑1 (MQ=255)                                                                       tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAaaaa                                                                                                                                 >  2:60833/1‑55 (MQ=255)                                                                       tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAAt                                                                                                                              <  2:1320528/58‑1 (MQ=255)                                                                       tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAAt                                                                                                                              >  1:1320528/1‑58 (MQ=255)                                                                       tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTAt                                                                                                              <  2:248692/74‑1 (MQ=255)                                                                       tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc                                                            >  1:291670/1‑124 (MQ=255)                                                                       tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTcc                                                            <  2:291670/124‑1 (MQ=255)                                                                       tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGt                                              >  1:117823/1‑138 (MQ=255)                                                                       tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGt                                              <  2:117823/138‑1 (MQ=255)                                                                       tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACACATCAAAAAATGCGTCATGAGTAGTAt                                                                                                              >  1:248692/1‑74 (MQ=255)                                                                         ccGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTaa                                                                          >  2:791500/1‑108 (MQ=255)                                                                         ccGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTaa                                                                          <  1:791500/108‑1 (MQ=255)                                                                                aTGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCAt                                    <  1:859178/139‑1 (MQ=255)                                                                                    aaCACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCa                                <  2:404225/139‑1 (MQ=255)                                                                                         aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAAt                                                                                                                              <  2:454895/40‑1 (MQ=255)                                                                                         aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAAt                                                                                                                              >  1:454895/1‑40 (MQ=255)                                                                                         aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTg                                                                                                      >  2:684146/1‑64 (MQ=255)                                                                                         aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTg                                                                                                      <  1:684146/64‑1 (MQ=255)                                                                                         aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCtata                                                   <  2:1015461/115‑1 (MQ=255)                                                                                         aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATg                           >  1:997548/1‑139 (MQ=255)                                                                                         aGCCGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGGTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCtata                                                   >  1:1015461/1‑115 (MQ=255)                                                                                           ccGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             <  1:1322945/103‑1 (MQ=255)                                                                                           ccGAATTAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             >  2:1322945/1‑103 (MQ=255)                                                                                                ttAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTg                                                                                                      >  1:1132350/1‑57 (MQ=255)                                                                                                ttAAGAAGAGAAATGTCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTg                                                                                                      <  2:1132350/57‑1 (MQ=255)                                                                                                               tCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGt                                                                            <  2:332561/68‑1 (MQ=255)                                                                                                               tCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGt                                                                            >  1:332561/1‑68 (MQ=255)                                                                                                               tCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGAt              <  1:136070/130‑1 (MQ=255)                                                                                                               tCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGAt              >  2:136070/1‑130 (MQ=255)                                                                                                               tCGCACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTgc     >  1:997106/1‑139 (MQ=255)                                                                                                                  cACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             <  1:1325026/80‑1 (MQ=255)                                                                                                                  cACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTc                                                             >  2:1325026/1‑80 (MQ=255)                                                                                                                  cACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTgcggc  >  1:2909/1‑139 (MQ=255)                                                                                                                  cACTCATAAAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTgcggc  >  2:116739/1‑139 (MQ=255)                                                                                                                          |                                                                                                                                   CTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC  >  NC_000913/4604081‑4604332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑