Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 477,435 T→C T395A (ACT→GCT)  ylaB ← putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913477,4350TC100.0% 201.8 / NA 55T395A (ACT→GCT) ylaBputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

CCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATGCTGACG  >  NC_000913/477312‑477567
                                                                                                                           |                                                                                                                                    
ccTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  2:1133230/139‑1 (MQ=255)
ccTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  2:515185/139‑1 (MQ=255)
  tGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtct                                                                                                                     >  1:538933/1‑139 (MQ=255)
   gTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctg                                                                                                                    >  2:225407/1‑139 (MQ=255)
   gTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  2:9784/1‑136 (MQ=255)
   gTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  1:1114698/1‑136 (MQ=255)
   gTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  2:1114698/136‑1 (MQ=255)
   gTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  1:9784/136‑1 (MQ=255)
    tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt                                                                                                                   >  2:1097160/1‑139 (MQ=255)
    tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  1:1191018/135‑1 (MQ=255)
    tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  2:1191018/1‑135 (MQ=255)
            ttAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  2:896957/127‑1 (MQ=255)
            ttAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  1:896957/1‑127 (MQ=255)
              aaaCTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTaa                                                                                                         >  2:278799/1‑139 (MQ=255)
               aaCTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAAc                                                                                                        <  1:278799/139‑1 (MQ=255)
                     aaTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                            <  1:985533/129‑1 (MQ=255)
                     aaTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                            >  2:985533/1‑129 (MQ=255)
                     aaTACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATa                                                                                                  >  2:29929/1‑139 (MQ=255)
                            cGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  2:1233417/1‑111 (MQ=255)
                            cGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  1:1233417/111‑1 (MQ=255)
                             gTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgattga                                                                                          <  1:29929/139‑1 (MQ=255)
                               aCCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgatt                                                                                            >  1:497946/1‑135 (MQ=255)
                               aCCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgatt                                                                                            <  2:497946/135‑1 (MQ=255)
                                     atcatcAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCa                                                                                  <  1:1097160/139‑1 (MQ=255)
                                        atcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  2:922670/99‑1 (MQ=255)
                                        atcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  1:922670/1‑99 (MQ=255)
                                                 aaTTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGtt                                                                                                                                 >  1:766053/1‑80 (MQ=255)
                                                 aaTTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGtt                                                                                                                                 <  2:766053/80‑1 (MQ=255)
                                                 aaTTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGc                                                                      <  1:962733/139‑1 (MQ=255)
                                                   tttCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCgg                                                                    <  1:225407/139‑1 (MQ=255)
                                                       aTGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGAtt                                                                >  2:1339504/1‑139 (MQ=255)
                                                           cccGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGAtttttt                                                            >  1:603623/1‑139 (MQ=255)
                                                              gCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCa                                                                                  <  2:562061/114‑1 (MQ=255)
                                                              gCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCa                                                                                  >  1:562061/1‑114 (MQ=255)
                                                              gCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCg                                                         >  1:918115/1‑139 (MQ=255)
                                                                 tCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAgg                                                      <  1:1339504/139‑1 (MQ=255)
                                                                         gCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACg                                              <  2:603623/139‑1 (MQ=255)
                                                                          cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGATCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACgg                                             >  2:951411/1‑139 (MQ=255)
                                                                               aaTTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTaa                                                                                                         >  1:308812/1‑74 (MQ=255)
                                                                               aaTTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTaa                                                                                                         <  2:308812/74‑1 (MQ=255)
                                                                                      ggggCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                            <  1:485355/64‑1 (MQ=255)
                                                                                      ggggCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                            >  2:485355/1‑64 (MQ=255)
                                                                                         gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  1:1202386/1‑50 (MQ=255)
                                                                                         gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  2:1202386/50‑1 (MQ=255)
                                                                                            cTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  1:1123656/47‑1 (MQ=255)
                                                                                            cTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  2:1123656/1‑47 (MQ=255)
                                                                                                  ttCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  1:1270238/1‑41 (MQ=255)
                                                                                                  ttCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  2:1270238/41‑1 (MQ=255)
                                                                                                         cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                            >  2:524115/1‑45 (MQ=255)
                                                                                                         cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                            <  1:524115/45‑1 (MQ=255)
                                                                                                         cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGAtt                                       >  2:1079808/1‑114 (MQ=255)
                                                                                                         cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGAtt                                       <  1:1079808/114‑1 (MQ=255)
                                                                                                                    cgTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    <  2:287514/42‑1 (MQ=255)
                                                                                                                    cgTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    >  1:287514/1‑42 (MQ=255)
                                                                                                                     gTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATGCTGACg  >  1:962450/1‑139 (MQ=255)
                                                                                                                           |                                                                                                                                    
CCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATGCTGACG  >  NC_000913/477312‑477567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑